Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Bojungikgitang Inhibits LPS Plus $Interferon-{\gamma}-induced$ Inflammatory Mediators in RAW 264.7 Macrophages

보중익기탕(補中益氣湯)의 Lipopolysaccharide와 $Interferon-{\gamma}$에 의해 유도되는 염증성 매개물에 대한 억제 효과(效果)

  • Jang Seon-Il (Immunopia Research Laboratory of Molecular and Cellualr Immunology) ;
  • Kim Hyung-Jin (Immunopia Research Laboratory of Molecular and Cellualr Immunology) ;
  • Kim Young-Jun (Immunopia Research Laboratory of Molecular and Cellualr Immunology) ;
  • Pae Hyun-Ock (Medical Resources Research Center, Wonkwang University) ;
  • Chung Hun-Taeg (Immunopia Research Laboratory of Molecular and Cellualr Immunology) ;
  • Yun Yong-Gab (Immunopia Research Laboratory of Molecular and Cellualr Immunology) ;
  • Jeong Ok-Sam (College of Pharmacy, Wonkwang University) ;
  • Kim Youn-Chul (Immunopia Research Laboratory of Molecular and Cellualr Immunology)
  • 장선일 (임뮤노피아 부설연구소) ;
  • 김형진 (임뮤노피아 부설연구소) ;
  • 김용준 (임뮤노피아 부설연구소) ;
  • 배현옥 (원광대학교 의약자원연구센터) ;
  • 정헌택 (임뮤노피아 부설연구소) ;
  • 윤용갑 (임뮤노피아 부설연구소) ;
  • 정옥삼 (원광대학교 약학대학 생약학교실) ;
  • 김윤철 (임뮤노피아 부설연구소)
  • Published : 2003.06.20
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Abstract

Bojungikgitang is the water extracts prepared from Ginseng Radix, Astragali Radix, Angelicae gigantis Radix, Astractylodis Rhizoma alba, Aurantii nobilis Pericarpium, Glycyrrhizae Radix, Bupleuri Radix, Cimicifugae Rhizoma, which has been used for the treatment of indigestion, and immunological disease in oriental countries. In this study, the effects of Bojungikgitang on the productions of nitiric oxide (NO) and prostaglandin $E_2\;(PGE_2)$, and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were examined using RAW 264.7 macrophages activated with $interferon-{\gamma}\;(IFN-{\gamma})$ plus lipopolysaccharide (LPS). Bojungikgitang (10-400 ${\mu}$g/ml) per se had no cytotoxic effect in unstimulated macrophages, but this compound dose-dependently reduced the release of NO and $PGE_2$ caused by stimulation of $LPS/IFN-{\gamma}$. The levels of iNOS and COX-2 protein were markedly suppressed by the treatment with Bojungikgitang in a concentration dependent manner. Moreover, Bojungikgitang also attenuated the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (1L)-1${\beta}$ and IL-6 in LPS-stimulated RAW 264.7 macrophages. These results suggest that Bojungikgitang decreases the NO and $PGE_2$ production in macrophages by inhibiting iNOS and COX-2 expression and these properties may contribute to the anti-inflammatory activity of Bojungikgitang.

Keywords

References

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