Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지

DEVELOPMENT OF MICROPOROUS CALCIUM PHOSPHATE COATED NERVE CONDUIT FOR PERIPHERAL NERVE REPAIR

말초신경 재건을 위한 인회석 박막 코팅 미세공성 신경재생관(nerve conduit)의 개발

  • Lee, Jong-Ho (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Hwang, Soon-Jeong (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Choi, Won-Jae (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Soung-Min (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Nam-Yeol (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Lee, Eun-Jin (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Ahn, Kang-Min (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Myung, Hoon (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Seo, Byoung-Moo (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Choi, Jin-Young (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Choung, Pill-Hoon (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Myung-Jin (Dept. of Oral & Maxillofacial Surgery, College of Dentistry and Dental Research Institute, Seoul National University) ;
  • Kim, Hyun-Man (Dept. of Oral Anatomy, College of Dentistry and Dental Research insititue, Seoul National University)
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 황순정 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 최원재 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김성민 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김남열 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 이은진 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 안강민 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 명훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 서병무 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 최진영 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 정필훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김명진 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김현만 (서울대학교 치과대학 구강해부학교실)
  • Published : 2003.06.30
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Abstract

This study was performed to develop a useful nerve conduit which provides favorable environment for Schwann cell viability and proliferation. Milipore membrane of $0.45{\mu}m$ pore size was selected because it permits nutritional inflow from the outside of the conduit and prevents from invading the fibrotic tissue into the conduit. The membrane was rolled and sealed to form a conduit of 2mm diameter and 20mm length. To improve the axonal regeneration and to render better environment for endogenous and exogenous Schwann cell behaviour, the microgeometry and surface of conduit was modified by coating with thin film of calcium phosphate. Cellular viability within the conduit and attachment to its wall were assessed with MTT assay and SEM study. Milipore filter conduit showed significantly higher rate of Schwann cell attachment and viability than the culture dish. However, the reverse was true in case of fibroblast. Coating with thin film of low crystalline calcium phosphate made more favorable environment for both cells with minimal change of pore size. These findings means the porous calcium phosphate coated milipore nerve conduit can provide much favorable environment for endogenous Schwann cell proliferation and exogenous ones, which are filled within the conduit for the more advanced strategy of peripheral nerve regeneration, with potential of reducing fibrotic tissue production.

Keywords

References

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