Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Inhibitory effects of ethanol extract of Atractylodis Rhizoma on melanogenesis in B16/F10 melanoma cells

  • Chun, Hyun-Ja (Division of Natural Sciences, Wonkwang University) ;
  • Lee, Jeong-Ho (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Eun-Young (Department of Skin and Care, Kimcheon Science College) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • Published : 2003.05.30
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Abstract

The inhibitory effect of Atractylodis Rhizoma alba extract(AM) on melanogenesis was studied by using B16/F10 melanoma in culture. Cells were cultured in the presence of various concentrations of AM for 48 hrs, and the experiment of total melanin content as a final product and activity of tyrosinase, a key enzyme, in melanogenesis. AM significantly inhibited tyrosinase activity, and melanin content in a dose-dependent manner. These results show that Atractylodes macrocephala extract could be developed as skin whitening components of cosmetics.

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References

  1. Chen ZL. (1987) The acetylenes from Artractylodes macrocephala, Planta Medica 53. 493-494. https://doi.org/10.1055/s-2006-962780
  2. Chen ZL, Cao WY, Zhou ex, Wichtl M. (1997) A sesquiterpene lactam from Artractylodes macrocephala. Phytochemistry 45, 765-767. https://doi.org/10.1016/S0031-9422(97)00036-8
  3. Ernst P, Ding YH. (1987) Handbuchen der Traditionellen Chinesischen Heilpflanzen, Karl F. Haug, Berlin, pp.197-198.
  4. Endo K, Taguchi T, Taguchi F, Hikino H, Yamahara T, Fujimura H. (1979) Antiinflammatory principles of atractylodes rhizomes. Chemistry and Pharmacology Bulletin 27, 2954-2958. https://doi.org/10.1248/cpb.27.2954
  5. Hosoi I, Abe E, Suda T, Kuroki T. (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 a-25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45, 1474-1478.
  6. Hearing VJ, Jimenez M. (1987) Mammalian tyrosinase the critical regulatory control point in melanocyte pigment. Int. J. Biochem., 19, 1141-1145. https://doi.org/10.1016/0020-711X(87)90095-4
  7. Im S, Moro O, Peng F. (1998) Activation of the cAMP pathway by a-melano-tropin mediates the rest of human melanocytes to ultraviolet B radiation. Cancer Res. 58, 47-51.
  8. Korner AM, Pawelek (1980) DOPAchrome conversion a possible control point in melanin biosynthesis. J. Invest. Dermatol. 75, 192-196. https://doi.org/10.1111/1523-1747.ep12522650
  9. Lerner AB, McGuire JS. (1961) Effect of alphaandbeta-melanocyte stimulating hormone on the skin color of man. Nature 189, 176-179. https://doi.org/10.1038/189176a0
  10. Lin YC, Jin T, Wu XY, Huang ZQ, Fan JS. (1997) A novel bisesquiterpenoid, biatractylolide, from the chinese herbal plant atractylodes macrocephala, 60, 27-28. https://doi.org/10.1021/np9603582
  11. Luger TA, Scholzen T, Grabbe S. (1997) The Role of amelanocyte stimulating hormone in cutaneous biology. Invest. Dermatol. Symp. Proc. 2,87.
  12. Matinez-Esparza M. (1998) Mechanisms and melanogenesis inhibition by tumor necrosis factor-a in B16/F10 mouse melanoma cells. Eur. J. Biochem. 225, 139-141.
  13. Mosmann T. (1983) Rapid colorimetric assay for the cellular growth and survival application to proliferation and cytotoxic assay. J. Immun. Methods 65, 55-58. https://doi.org/10.1016/0022-1759(83)90303-4
  14. Ranson M, Posen S, Manson RS. (1988) Human melanocytes as a target tissue for hormone: in vitro studies with 1 a-25 dihydroxyvitamine D3, a-melanocyte stimulating hormone and beta estradiol. J. Invest. Dermatol. 91, 593-597. https://doi.org/10.1111/1523-1747.ep12477126

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