유색미 에탄올 추출물의 품종간 항산화 활성 변이

Varietal Difference in Antioxidative Activity of Ethanolic Extracts from Colored Rice Bran

  • Nam, Seok-Hyun (Department of Natural Science, Ajou University) ;
  • Chang, Su-Min (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Kang, Mi-Young (Department of Food Science and Nutrition, Kyungpook National University)
  • 발행 : 2003.02.28

초록

갈색에서부터 흑자색까지의 색상을 가지는 국.내외에서 수집한 23종류 유색미의 항산화 활성, 색소체 함량의 변기 및 상관성을 검정하였다. 유색미 에탄올 추출물 시료가 나타내는 환원력은 일반미 품종인 추청에 비해서 거의 모든 품종에서 높게 나타났으며, 환원력이 특히 높은 품종은 LK 1-3-6-12-1-1, Elwee, DZ 78, Jumlalocal-1, SC-45 등이었다. 전자공여능이 높은 품종은 HP 883-1-1-1-B-1-1, HP 833-1-3-1-1-1, LK-2-7-12-1-1, DZ 78 등이었고 hydroxy radical 소거활성이 큰 품종은 DK-1, IR 1544-38-2-2-1-2-2, SC-5, SC-45 등이었으며, LK 2-7-12-1-1은 오히려 산화촉진효과를 나타내는 품종이었다. 자동산화에 의한 지질 과산화물 형성을 강하게 억제하는 품종들은 RGS No. 336, LK IB-2-1-1, LK 1B-4-12-1-1, LK 1A-2-12-1-1, LK 2-7-12-1-1, HP 833-1-1-1-B-1-1등이었고 Elwee, Jumlalocal-1, SC-45 등의 품종은 산화적인 활성을 나타내고 있었다. 23품종의 유색미 중에서 색소체의 함량이 가장 많은 품종은 Elwee이었으며, 색소체 함량은 환원력과 정의 상관성이 있었고, 추출물이 고농도로 처리되었을 때 높은 환원력을 보인 유색미 품종은 linoleic acid 자동산화반응에서 지질 과산화를 촉진하는 경향이 있었다. 유색미 23품종 중에서 SC-5는 모든 반응계에서 비교적 높은 항산화 활성을 나타내었다.

Interrelation between the antioxidative activities of 70% ethanol extracts from bran fraction of 23 kinds of colored rice and chuchung as a control were examined. Antioxidative activities were evaluated by assaying reducing power, electron-donation ability to DPPH free radical, scavenging activity of hydroxy radical $({\cdot}OH)$ generated through Fenton reaction and inhibitory activity on lipid peroxidation using linoleic acid autoxidation system, respectively. Among 24 varieties of colored rice LK 1-3-6-12-1-1 had the strongest reducing power followed by Elwee, DZ 78, Jumlalocal-1 and SC-45 in decreasing order. The electron-donating ability to DPPH radical was higher in order of HP 883-1-1-1-B-1-1, HP 833-1-3-1-1-1, LK 2-7-12-1-1 and DZ 78. The hydroxy radical scavenging activity was higher in order of DK-1, IR 1544-38-2-2-1-2-2, SC-5 and SC-45 but LK 2-7-12-1-1 had oxidative effect. In the liaoleic acid autoxidation model system, RGS No 336, LK 1B-2-1-1, LK 1B-4-12-1-1, LK 1A-2-12-1-1, LK 2-7-12-1-1 and HP 883-1-1-1-B-1-1 exhibited strong antioxidative activities but Elwee, Jumlalocal-l and SC-45 showed to have oxidative effects. The rice variety of highest pigment content was Elwee and the next were RGS-No 336, IR 1544-38-2-2-1-2-2 and SC-5 with the order of higher content. The reducing power was correlated with the quantity of the pigment in the ethanolic extract of rice bran and SC-5 showed relatively high antioxidative activity in every results of antioxidative activity tests.

키워드

참고문헌

  1. Chang, S. M., Kim, K. H. and Kang, M. Y. (2001) Varietal difference in processing and sensory characteristics of Misutkaru in rice. Korean J. Breed. 33, 73-79
  2. Shin, S. Y, Sung, Y. M. and Kang, M. Y. (2001)Saccharification and sensory characteristics of Shikhe madefrom glutinous rice varieties. J. East Asian Soc. Dietary Life.11, 11-18
  3. Choi, H. C. and Oh, S. K. (1996) Diversity and function of pigments in colored rice. J. Crop Sci. 41, 1-9
  4. Yoon, J. M., Hahn, T. R. and Yoon, H. H. (1998) Etfect of copigmentation on the stability of anthocyanins from a Koreanpigmented rice variety. Korean J. Food Sci. Technot. 30, 733- 738
  5. Oh, S. K., Choi, H. C., Cho, M. Y. and Kim, S. U. (1996)Extraction method of anthocyanin and tannin pigments incolored rice. Agri. Chem. and Biotechnot. 39, 327-331
  6. Yoon, J. M., Cho, M. H., Hahn, T. R., Paik, Y. S. and Yoon,H. H. (1997) Physicochemical stability of anthocyanins from a Korean pigmented rice variety as natural food colorants. Korean J. Food Sci. Technot. 29, 211-217
  7. Cho, J. M., Yoon, H. H. and Han, T. R. (1996) Thermalstability of the major color components, cyanidin 3-g1ucoside, from a Korean pigmented rice. Agri. Chem. and Biotechnot. 38, 581-583
  8. Ramarathnam, N., Osawa, T, Namiki, M. and Kawakishi.S. (1988) Chemical studies on novel andoxidants I. Isolation, & actionation and partial characterization. J. Agric. Food Chem36, 727-732
  9. Ramarathnam, N., Osawa, T, Namiki, M. and Kawakishi, S.(1989) Chemical studies on novel andoxidants n. Identification of isovitexin C-glycosyl flavonoid. J. Agric. Food Chem. 37, 316-319 https://doi.org/10.1021/jf00086a009
  10. Kang, M. Y, Choi, Y. H. and Nam, S. H. (1996) Inhibitory mechanism of colored rice bran extract against mutagenicitinduced by chemical mutagen Mitomycin C. Agri. Chem. andBiotechiwI. 39, 424-429
  11. Nam, S. H. and Kang, M. Y. (1997) In vitro inhibitory effect of colored rice bran extracts carcmogenicity. Agri. Chem. and Biotechnot. 40, 307-312
  12. Choi, S. W., Nam, S. H. and Choi, H. C. (1996) Antioxidative activity of ethanolic extracts of rice brans. Food Sci. Biotechml. 5, 305-309
  13. Kim, D. W., Eun, J. B. and Rhee, C. 0. (1998) Cooking condition and textural changes of cooked rice added with black rice. Korean J. Food Sci. Techmt. 30, 562-568
  14. Kim, S. S., Kim, S. Y. and Lee, W. J. (1998) Chracteristics of germinated colored dee as a potential raw material of Shikhe. Korean J. Food Sci. Technot. 30, 1092-1096
  15. Oyaizu, M. (1986) Studies on products of browning reaction. Antioxidative activities of products of browning reactionprepares from glucosamine. Jpn. J. Nutri. 44, 307-315
  16. Osawa, T. and Namiki, M. (1981) A novel type antioxidant isolated from leaf wax of Eucalyptus leaves. Agric. Biot. Chem. 45, 735-740 https://doi.org/10.1271/bbb1961.45.735
  17. Unno, T, Sakane, I., Masumizu, T, Kohono, M. and Kakuda,T. (1997) Antioxidative activity of water extract of Lagerstroernia speciosa leaves. Biosci. Biotech. Biochem. 61, 1772-1774 https://doi.org/10.1271/bbb.61.1772
  18. Garbor, E. (1988) Possible biological role of some anthocyanins in food. Butt. Liaison-Group Potyphenots. 14, 130-135
  19. Meunier, M. T, Duroux, E. and Bastide, P. (1989) Andoxidant activity of procyanidol oligomers and anthocyanins with regard to superoxide anion and lipid peroxidadon. Ptant Med. Phytother. 23, 261-21
  20. Tsuda, T., Oshima, K., KawasaId, S. and Osawa, T. (1994)Andpxidadve pigments isolated from the seeds of Phaseolus vulgans L. J. Agric. /Food Chem. 42, 248-252 https://doi.org/10.1021/jf00038a004
  21. Choi, S. W., Kang, W. W., Osawa, T. and Kawaldshi, S. (1994) Antioxi dative activity of crysanthemin in black lice hulls. FoodSci. Biotechnot. 3, 233-238
  22. Tsuda, T, Watanabe, M., Oshima, K., Norinobu, S., Choi, S. W., Kawasaki, S. and Osawa, T. (1994) Antioxidative acdvity of the anthocyanin pigments cyanidin 3-0-(3-g1ucoside and cyanidin. J. Agric. Food Chem. 42, 2407-2410 https://doi.org/10.1021/jf00047a009