Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Microbial Immobilization, Characterization and Isolation of Nitrogen Oxidizing Bacteria

암모니아 및 아질산성 질소 산화세균의 분리 및 특성

  • Lee, Yong-Seok (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Yoo, Ju-Soon (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Chung, Soo-Yeol (Dept. of Food Science, Dongju College) ;
  • Park, Choon-Soo (E & P Corp.) ;
  • Choi, Yong-Lark (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
  • Published : 2003.02.28
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Abstract

Abstract: In order to improve the system for biological nitrogen oxidizing process in sewage and wastewater, a bacterium having high abilities to oxidize of nitrogen was isolated from wastewater and polluted soils. The strain was identified to Bacillus sp. CH-N, based on the physiological and biochemical properties. Characteristics and oxidizing ability of both ammonia and nitrite were examined for the strain, Bacillus sp. CH-N. The strain showed the oxidizing rate about 80% to 90% on the sewage and wastewater after 48 h culture. The nitrogen oxidizing rate was increased in proportion to the initial concentration of glucose. The microorganism, Bacillus sp. CH-N cell immobilized on ceramic carrier were evaluated for the oxidation of ammonia in culture media.

본 연구는 폐수 중의 질소 제거를 위한 생물학적 처리용 미생물 개발을 위한 목적으로 질소의 산화 능력이 뛰어난 균주를 분리하였다. 분리된 세균 중에서 질소 산화능과 생육 속도가 뛰어난 CH-N 균주를 선별하였으며, 생리, 생화학적 특성 조사에 의해 Bacillus sp로 추정되어 Bacillus sp. CH-N이라 명명하였다. 분리 균주는 0.5% glucose가 포함된 초기 pH가 7,0인 암모니아 및 아질산성 질소 함유 배지에서 30시간 배양 후 각각 85%와 90%의 암모니아성과 아질산성 질소의 감소율을 나타내었다. 폐수 및 생활하수에 분리 균주를 이용한 결과, 수질 속의 암모니아성 질소가 단시간에 크게 감소시키는 효과를 확인하였다. 균주를 고정시킨 담체의 질소산화 효과를 시험하고자 Bacillus sp. CH-N을 고정시킨 세라믹 담체를 이용한 결과, 배양 2일 후에는 암모니아성 질소가 전부 제거되었다.

Keywords

References

  1. Yun, D. I., Lee, J. T, Kim, D. J. and Lee, K. Y. (1998) The effect of external carbon sources on batch denitrification process. Kor. J. Appt. MicrobioI. 26, 96-101
  2. Anderson, I. C. and Levine, J. S. (1986) Relative rates of nitric oxide and nitrous oxide production by nitrifiers, denitnSers, and nitrare respirers. AppI. Environ. Microbiot. 51, 938-945
  3. Fang, H. Y. and Chou, M. S. (1993) NitriScation of ammonia nitrogen in refinery wastewater. Water Research. 27, 1761-1765 https://doi.org/10.1016/0043-1354(93)90114-W
  4. Song, J. Y, Hwang, S. Y. and Kim, D. S. (2001) A Study on the denitriScadon characteristics of permeabilized Paracoccus denitrificans. Kor. J. Biotechnot. Bioeng. 16, 290-294
  5. Lee, S. I., Park, S. K. and Lee, W. H. (1994) Temperature dependency of denitnfication rate with addition of various electron donors, J. Kor. Soc. Emiron. Engineers 16, 677-683
  6. Nam, B. S., Ryu, W. R., Lee, Y. H., Kim, J. M. and Cho, MH. (1999) Isolation and characterization of ammonia and nitrite nitrogen oxidizing strains. Kor. J. BiotechnoI. Bioeng. 14, 76- 81
  7. Kuai, L. and Verstraete, W. 1998. Ammonium removal by the oxygen-limited autotrophic mtn6cation-demtiification system. Appt. Environ. MicmbioL 64, 4500-4506
  8. Won, C. H., Lee, M. H. and Yun, J. S. (1997) Simultaneous removal of nitrogen and organics in septic tank effluent using immobilized microorganisms for nitrification and denitrification. J. Kor. Soc. Environ. Eng. 19, 683-692
  9. Suh, K. H., Kim, B. J., Cho, M. C., Kim, Y. H. and Kim, S. G. (1998) Removal of ammonia-N by immobilized nitnfie rconsortium. Kor. J. Biotechmt. Bioeng. 13, 76-81
  10. Kheg, M. R. and Holt, J. G. (1984) In Bergey's manual of systematic bacteriology The Williams and Wilkins Co., Baltimore, USA
  11. APHA, AWWA, WEF. (1992) In Standard methods for the examination of water and wastewater (18th ed.) Washington D.C
  12. Nam, E. S., Choi, J. W., Cha, S. K. and Ahn, J. K. (2002) Isolation and identi6cation of thermostable P-glycosidase- producing microorganism from hot spring of volcanic area at Atagawa in Japan. Kor. J. Microbiot. Bitechnot. 30, 151-156