Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Analysis of Diversity of Panax ginseng Collected in Korea by RAPD Technique

RAPD 방법을 이용한 국내 수집 인삼 (Panax ginseng C. A. Meyer)의 다양성 분석

  • Seo, Sang-Deog (Chungnam Agricultural Research & Extension Services) ;
  • Yuk, Jin-Ah (College of Agric. & Life Sci., Chungnam Natl. Univ.) ;
  • Cha, Sun-Kyung (College of Agric. & Life Sci., Chungnam Natl. Univ.) ;
  • Kim, Hyun-Ho (Gumsan Agricultural Development & Technology Center) ;
  • Seong, Bong-Jae (Gumsan Agricultural Development & Technology Center) ;
  • Kim, Sun-Ick (Gumsan Agricultural Development & Technology Center) ;
  • Choi, Jae-Eul (College of Agric. & Life Sci., Chungnam Natl. Univ.)
  • Published : 2003.12.31
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Abstract

Genetic differences among nine land races of Korean ginseng (Panax ginseng C. A. Meyer) were examined using RAPD markers. Land races of Korean ginseng were collected from nine regions in Korea: Cheongwon, Guesan, Geumsan, Namwon, Pochun, Yangju, Yeoncheon, Yeongju. Out of 48 RAPD primers tested, 5 primers (OPA 7, OPA 13, URP 2, URP 3 and UBC 3) produced remarkable bands which showing polymorphisms among evaluated collections. Lower levels of genetic diversity were in detected same land races than among other land races. Genetic differences within and among land races indicate heterogeneity. These results indicate that cultivated ginseng in Korea is heterogeneous. Genetic similarity matrices of RAPD profiles were generated via coefficients of variation and the data were processed by the cluster analysis (UPGMA). When 90 collections were evaluated using selected 5 primers, those were clustered to 5 and 3 subgroups. These differences in genetic variation between land races of Korean ginseng implied the potential source for further breeding of Korean ginseng.

본 연구는 고려인삼 재래종의 유전적 차이를 RAPD marker로 평가하였다. 재래종은 우리나라 주요 인삼재배 단지인 괴산, 금산, 남원, 포천, 양주, 연천, 영주로부터 수집한 인삼에서 10개체를 임의 선발하여 사용하였다. 9개 지역의 90개체를 대상으로 RAPD분석을 한 결과 48개의 primer 중 OPA 7, OPA 13, URP 2, URP 3, UBC 3의 5개 primer가 재현성이 있고 재래종 내 개체간에도 다형성인 band를 보였다. 재래종 집단간보다 재래종 내에서 유전적 다양성이 낮았다. 재래종 집단 간 또는 재래종 집단내의 유전적 차이가 있다는 것은 이 집단들이 헤테로라는 것을 의미한다. 이러한 결과는 우리나라에서 재배중인 고려 인삼은 유전 자원의 혼합으로 헤테로라는 것을 암시한다. 선발된 5개의 primer를 이용하여 90개체를 집괴분석한 결과 국내 재래종 인삼은 5개군으로 그리고 3개의 아군으로 구분되었다. 국내 재래종 인삼은 유전적 변이가 크므로 인삼 육종을 위한 재료로 이용될 수 있을 것이다.

Keywords

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