Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Cnidii Rhizoma Water Extract on Chemopreventive Enzymes for Hepatocarcinoma

천궁 물추출물이 간암예방효소계에 미치는 영향

  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Kim, Han-Gyu (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 김한규 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소)
  • Published : 2003.12.30
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Abstract

Cnidii Rhizoma water extract (CRW) was tested for liver cancer chemopreventive potential by measuring the inhibition of phase I enzyme and benzo[a]pyrene-DNA adduct formation and induction of phase II detoxification enzymes. There was 17.0% inhibition in the activity of cytochrome P450 1A1 enzyme with the treatment of 150 mg/ml CRW. At concentration of 30 mg/ml CRW, the binding of $[^3H]B[a]P$ metablites to DNA of NCTC-clone 1469 cell was inhibited by 33.3%. CRW was potent inducer of quinone reductase (QR) and glutathione S-transferase (GST) activities in cultured murine hepatoma Hepalc1c7 cells. However, hepatic glutathione (GSH) level was not influenced by CRW. These findings suggest that CRW has chemopreventive potential of liver cancer by inhibiting cytochrome P450 1A1 activity and benzo[a]pyrene-DNA adduct formation and inducing QR and GST activities.

Keywords

References

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