생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Ames, Rec 및 umu Assay를 이용한 황기의 안전성평가

Evaluation of Safety with Astragali Radix : Ames, Rec and umu Assays

  • 손윤희 (동국대학교 의과대학 약리학교실) ;
  • 남경수 (동국대학교 난치병한양방치료연구소)
  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Nam, Kyung-Soo (Intractable Disease Research Center, Dongguk University)
  • 발행 : 2003.03.30
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초록

Water extract from Astragali Radix (AR) was tested for the safety using Ames, Bacillus subtilis Rec, and umu gene expression mutagenicity tests. Mutagenic activity in any assays we tested was not found. In Ames test, Salmonella typhimurium TA98 and TA 100 were used to identify mutagenic property, and the number of histidine revertants was measured. In the Recassay, Bacillus subtilis ${H-17(Rec^+)\;and\;M-45(Rec^-)}$ strains were used to test DNA damage activity. In the SOS umu test, Salmonella typhimurium TA1535 containing plasmid pSK1002 was used as a test strain, and we monitored the levels of umu operon expression by measuring the ${\beta}-galactosidase$ activity. From the results, there was no DNA damage and mutagenicity of AR. Hepatotoxicity of AR to female ICR mice was also monitored by the measurements of s-GOT, s-GPT, LDH activities after oral feeding for 15 days. AR was not shown any significant changes of s-GOT, s-GPT and LDH activities in mice sera.

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참고문헌

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