Natural Product Sciences

  • 제9권1호
  • /
  • Pages.13-17
  • /
  • 2003
  • /
  • 1226-3907(pISSN)
  • /
  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Hepatoprotective Effect of Coccinia indica Against $CCl_4$ Induced Hepatotoxicity

  • Rao, GM Mohana (Pharmacognosy and Ethnopharmacology division, National Botanical Research Institute) ;
  • Vijayakumar, M. (Pharmacognosy and Ethnopharmacology division, National Botanical Research Institute) ;
  • Rao, ChV (Pharmacognosy and Ethnopharmacology division, National Botanical Research Institute) ;
  • Rawat, AKS (Pharmacognosy and Ethnopharmacology division, National Botanical Research Institute) ;
  • Mehrotra, S. (Pharmacognosy and Ethnopharmacology division, National Botanical Research Institute)
  • 발행 : 2003.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The hepatoprotective effect of the ethanolic extract of Coccinia indica fruits in rats treated with carbon tetrachloride. In hepatotoxic rats, liver damage was studied by assessing parameters such as aspartate aminotransferase (AST), alanine aminotransferase (AlT), alkaline phosphatase (AIP) and gamma glutamyl transpeptidase (GGT) in serum, and concentrations of total proteins, total lipids, phospholipids, triglycerides and cholesterol in both serum and liver. The effect of co-administration of ethanolic extract on the above parameters was further investigated. Histopathological study of the liver in experimental animals was also undertaken. Hepatic damage as evidenced by a rise in the levels of AST, AIT, AIP and GGT in serum, and also changes observed in other biochemical parameters In serum and liver showed a tendency to attain near normalcy in animals co-administered with the extract. The normal values for AST (IU/L), AIP (IU/I), protein (g/100 ml) and total lipids (mg/100 ml) in serum (i.e.,20.24, 70.04, 5.72 and 135.54 respectively) were found to alter towards values 32.61, 127.11, 3.83 and 265.91 in hepatotoxic rats. These parameters Attained near normal values (I.e.,22.82, 79.30, 5.22 and 151.24 for AST, AIP protein and total lipids respectively) in ethanolic extract co-administered rats. Profound steatosis, ballooning degeneration and nodule formation observed in the hepatic architecture of $CCl_4$ treated rats were found to acquire near-normalcy in drug co-administered rats, thus corroborating the biochemical observations. Thus the study substantiates the hepatoprotective potential of ethanolic extract of Coccinia indica fruits.

키워드

참고문헌

  1. Abell, L. L., Levy, B. B., and Kendall, F. E., A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J. Biol. Chem. 195, 357-366 (1952)
  2. Dubey, G. P., Agrawal, A, and Dixit, S. P., Effects of Liv.52 on different biochemical parameters in alcoholic cirrhosis. Antiseptic, 91, 205-208 (1994)
  3. Farooq, S., Ahmad, I., Pathak, G. K., In vivo protective role of Koflet (anayurvedic preparation) against cellular toxicity caused by $CCl_{4}$ and flyash. J Ethnopharmocol, 58, 109-16 (1997) https://doi.org/10.1016/S0378-8741(97)00094-9
  4. Fring, C. S., and Dunn, R., A Colorimetric method of determination of total lipids based on sulfophosphate -vanillin reaction. American J. Clin. Pathol., 4, 53-89 (1970)
  5. Hossain, M. Z., Shibib, B. A., Rahman, R., Hypoglycemic effects of Coccinia indica: inhibition of key gluconeogenic enzyme, glucose-6-phosphatase: Ind. J. Exp. BioI, 30(5), 418-420 (1992)
  6. Kind, P. R. N. and King, A. J., Elimination of plasma phosphate by determination of hydrolysed phenol with aminoantipyrine. J. Clin. Pathol. 7, 322-326 (1954) https://doi.org/10.1136/jcp.7.4.322
  7. Kirtikar, K. R., and Basu, B. D., Indian Medicinal Plants, Vol II, Lalit Mohan Basu, Allhabad, 1933, pp 1151
  8. Kumar, G. P., Sudheesh, S., Vijayalakshmi, N. R., Hypoglycaemic effect of Coccinia indica: mechanism of action. Planta Med, 59(4), 330-332 (1993) https://doi.org/10.1055/s-2006-959693
  9. Lowry, O. H., Aosebrough, N. J., Farr, A L., and Randall, R. J., Protein measurement with Folin Phenol reagent. J. Bioi. Chem., 193, 265- 275(1951)
  10. Mitra, S. K., Venkataranganna, M. V., Sundaram, R., and Gopumadhavan, S., Protective effect of HD-03 a herbal formulation, against various hepatotoxic agents in rats. J Ethnopharmacol, 63, 181-186 (1998) https://doi.org/10.1016/S0378-8741(98)00088-9
  11. Nadkarni, A. K., Indian Materia Medica, Vol. I, Popular Book Depot, Bombay, 1954, pp. 300
  12. Naftalin, L., Markaret, S., Whitaker, J. F., and Tracey, D., A routine procedure for estimating serum gamma glutamyl transpeptldase activity. Clin. Chem. Acta, 26, 293-296 (1969) https://doi.org/10.1016/0009-8981(69)90381-7
  13. Ram, V. J., and Goel, A., Past and present scenario of hepatoprotectants. Curr. Med. Chem. 6, 217-254 (1999)
  14. Recknagel, R. O., Carbon tetrachloride hepatotoxicity. Pharmacol. Review, 19, 145-208 (1967)
  15. Recknagel, R. O., Glende, E. A., Jr Dolak, J. A., and Walter, A L., Mechanism of carbon tetrachloride toxicity. Pharmacol. Ther. 43, 139-154 (1989)
  16. Reitman, S. and Frankel, A. S., A colorimetric method for the determination of serum glutamic oxaloaceticand glutamic pyruvic transaminase. American J. Clin. Pathol. 28, 53-56 (1957)
  17. Seakins, A., and Robinson, O. S., The effect of administration of carbon tetrachloride on the formation of plasma lipoproteins in the rat. J. Biochem, 86, 401-407 (1963)
  18. Uday, B., Das, D., and Banerjee, K. R., Reactive oxygen species, Oxidative damage and pathogensis. Curr. Sci, 77, 658-666 (1999)
  19. Van, H. E., and Zilversmith, D. B., Determination of serum triglycerides. J. Lab. Clin Med, 50,152 (1957)
  20. Varley H, Practical Clinical Biochemistry. CBS Publishers and Distributors, New Delhi. pp. 319-320 (1988)