SM709, Ingredient of Antimelanogenic Bamboo Extract, Blocks Endothelin-1-induced $[Ca^{2+}]_i$ Increase in Human Melanocytes

  • Kim, Shin-Hee (Department of Physiology, College of Medicine, Chungnam National University) ;
  • Lee, Ki-Mu (Cosmetic Research Center, Department of Skin Research, Aekyung Industrial Corporation) ;
  • Kim, Hyo-Shin (Department of Physiology, College of Medicine, Chungnam National University) ;
  • Lee, Gyu-Seung (Department of Physiology, College of Medicine, Chungnam National University) ;
  • Jeon, Byeong-Hwa (Department of Physiology, College of Medicine, Chungnam National University) ;
  • Kim, Kwang-Jin (Department of Physiology, College of Medicine, Chungnam National University) ;
  • Park, Jin-Bong (Department of Physiology, College of Medicine, Chungnam National University)
  • 발행 : 2003.12.21

초록

Endothelins secreted from keratinocytes are intrinsic mitogens and melanogens of human melanocytes in UVB-induced hyperpigmentation. To elucidate the cellular mechanism of antimelanogenic activity of bamboo extract, the effects of three ingredients of bamboo extract on endothelin 1 (ET-1)-induced $Ca^{2+}$ mobilization were investigated in cultured human melanocytes. ET-1 receptors in human melanocytes were characterized by using specific antagonist, and ET-1 was found to increase intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) by activating ET-B receptor. SM709 (1,2-O-diferulyl-glycerol), an ingredient of bamboo extract, inhibited ET-1-induced $[Ca^{2+}]_i$ increase in a concentration- and time-dependent manner, although another ingredients SM707 and SM708 had no effect on ET-1-induced $[Ca^{2+}]_i$ increase in human melanocytes. SM709 ($100{\mu}M$), however, did not affect $[Ca^{2+}]_i$ increase induced by thapsigargin and caffeine, suggesting that SM709 has no effect on the $Ca^{2+}$ store in melanocytes. Furthermore, SM709 did not affect $[Ca^{2+}]_i$ increase induced by LPA or ATP, known as G protein-mediated PLC activators like ET-1. Taken together, it is suggested that SM709 antagonizes ET-1-induced transmembrane signaling through ET-B receptor, which maybe a possible underlying mechanism of antimelanogenic activity of bamboo extract in human melanocytes.

키워드

참고문헌

  1. Amiel J, Attie T, Jan D, Pelet A, Edery P, Bidaud C, Lacombe D, Tam P, Simeoni J, Flori E, Nihoul-Fekete C, Munnich A, Lyonnet S. Heterozygous endothelin receptor B (EDNRB) mutations in isolated Hirschsprung disease. Hum Mol Genet 5: 355-357, 1996 https://doi.org/10.1093/hmg/5.3.355
  2. Baynash AG, Hosoda K, Giaid A, Richardson JA, Emoto N, Hammer RE, Yanagisawa M. Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell 79: 1277-1285, 1994 https://doi.org/10.1016/0092-8674(94)90018-3
  3. Berking C, Takemoto R, Satyamoorthy K, Elenitsas R, Herlyn M. Basic fibroblast growth factor and ultraviolet B transform melanocytes in human skin. Am J Pathol 158: 943-953, 2001 https://doi.org/10.1016/S0002-9440(10)64041-2
  4. Carsberg CJ, Jones KT, Sharpe GR, Friedmann PS. Intracellular calcium modulates the responses of human melanocytes to melanogenic stimuli. J Dermatol Sci 9: 157-164, 1995 https://doi.org/10.1016/0923-1811(94)00372-L
  5. Cho JH, Lee KM, Kim NS, Kang WH. The effects of bamboo extract on Human melanocytes and B16 Melanoma Cells in vitro. 4th Scientific Conference of the Asian Societies of Cosmetic Scientists- Ball, Indonesia 7-9, 1999
  6. Dissanayake NS, Mason RS. Modulation of skin cell functions by transforming growth factor-$\beta$1 and ACTH after ultraviolet irradiation. J Endocrinol 159: 153-163, 1998 https://doi.org/10.1677/joe.0.1590153
  7. Edery P, Attie T, Amiel J, Pelet A, Eng C, Hofstra RM, Martelli H, Bidaud C, Munnich A, Lyonnet S. Mutation of the endothelin- 3 gene in the Waardenburg-Hirschsprung disease (Shah- Waardenburg syndrome). Nat Genet 12: 442-444, 1996 https://doi.org/10.1038/ng0496-442
  8. Eisinger M, Marko O. Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proc Natl Acad Sci USA 79: 2018-2022, 1982 https://doi.org/10.1073/pnas.79.6.2018
  9. Hachiya A, Kobayashi A, Ohuchi A, Takema Y, Imokawa G. The paracrine role of stem cell factor/c-kit signaling in the activation of human melanocytes in ultraviolet-B-induced pigmentation. J Invest Dermatol 116: 578-586, 2001 https://doi.org/10.1046/j.1523-1747.2001.01290.x
  10. Ihara M, Ishikawa K, Fukuroda T, Saeki T, Funabashi K, Fukami T, Suda H, Yano M. In vitro biological profile of a highly potent novel endothelin (ET) antagonist BQ-123 selective for the ETA receptor. J Cardiovasc Pharmacol 20(Suppl): S11-S14, 1992
  11. Imokawa G, Miyagishi M, Yada Y. Endothelin-1 as a new melanogen: coordinated expression of its gene and the tyrosinase gene in UVB-exposed human epidermis. J Invest Dermatol 105: 32-37, 1995 https://doi.org/10.1111/1523-1747.ep12312500
  12. Imokawa G, Yada Y, Miyagishi M. Endothelins secreted from human keratinocytes are intrinsic mitogens for human melanocytes. J Biol Chem 267: 24675-24680, 1992
  13. Imokawa G, Yada Y, Kimura M. Signalling mechanisms of endothelin-induced mitogenesis and melanogenesis in human melanocytes. Biochem J 314(Pt 1): 305-312, 1996 https://doi.org/10.1042/bj3140305
  14. Ishikawa K, Ihara M, Noguchi K, Mase T, Mino N, Saeki T, Fukuroda T, Fukami T, Ozaki S, Nagase T, Nishikibe M, Yano M. Biochemical and pharmacological profile of a potent and selective endothelin B-receptor antagonist, BQ-788. Proc Natl Acad Sci USA 91: 4892-4896, 1994 https://doi.org/10.1073/pnas.91.11.4892
  15. Kang HY, Kang WH, Lee C. Endothelin-B receptor-mediated Ca2$^+$ signaling in human melanocytes. Pflugers Arch 435: 350-356, 1998
  16. Khiroug L, Giniatullin R, Sokolova E, Talantova M, Nistri A. Imaging of intracellular calcium during desensitization of nicotinic acetylcholine receptors of rat chromaffin cells. Br J Pharmacol 122: 1323-1332, 1997 https://doi.org/10.1038/sj.bjp.0701518
  17. Liu DM, Katnik C, Stafford M, Adams DJ. P2Y purinoceptor activation mobilizes intracellular Ca2$^+$ and induces a membrane current in rat intracardiac neurones. J Physiol 526(Pt 2): 287- 298, 2000 https://doi.org/10.1111/j.1469-7793.2000.00287.x
  18. Murthy KS, Makhlouf GM. Coexpression of ligand-gated $P_{2X}$ and G protein-coupled $P_{2Y}$ receptors in smooth muscle. Preferential activation of $P_{2Y}$ receptors coupled to phospholipase C (PLC)-$\beta$1 via G${\alpha}_{q/11 }$and to PLC-$\beta$3 via G$\beta$${\gamma}_{i3}$. J Biol Chem 273: 4695-4704, 1998 https://doi.org/10.1074/jbc.273.8.4695
  19. Noh SJ, Kim MJ, Shim S, Han JK. Different signaling pathway between sphingosine-1-phosphate and lysophosphatidic acid in Xenopus oocytes: functional coupling of the sphingosine-1-phosphate receptor to PLC-x$\beta$ in Xenopus oocytes. J Cell Physiol 176: 412-423, 1998 https://doi.org/10.1002/(SICI)1097-4652(199808)176:2<412::AID-JCP20>3.0.CO;2-3
  20. Pietruck F, Busch S, Virchow S, Brockmeyer N, Siffert W. Signalling properties of lysophosphatidic acid in primary human skin fibroblasts: role of pertussis toxin-sensitive GTP-binding proteins. Naunyn Schmiedebergs Arch Pharmacol 355: 1-7, 1997 https://doi.org/10.1007/PL00004906
  21. Puffenberger EG, Kauffman ER, Bolk S, Matise TC, Washington SS, Angrist M, Weissenbach J, Garver KL, Mascari M, Ladda R. Identity-by-descent and association mapping of a recessive gene for Hirschsprung disease on human chromosome 13q22. Hum Mol Genet 3: 1217-1225, 1994 https://doi.org/10.1093/hmg/3.8.1217
  22. Sakurai T, Yanagisawa M, Takuwa Y, Miyazaki H, Kimura S, Goto K, Masaki T. Cloning of a cDNA encoding a non-isopeptideselective subtype of the endothelin receptor. Nature 348: 732-735, 1990 https://doi.org/10.1038/348732a0
  23. Tada A, Suzuki I, Im S, Davis MB, Cornelius J, Babcock G, Nordlund JJ, Abdel-Malek ZA. Endothelin-1 is a paracrine growth factor that modulates melanogenesis of human melanocytes and participates in their responses to ultraviolet radiation. Cell Growth Differ 9: 575-584, 1998
  24. Thastrup O, Cullen PJ, Drobak BK, Hanley MR, Dawson AP. Thapsigargin, a tumor promoter, discharges intracellular Ca2$^+$ stores by specific inhibition of the endoplasmic reticulum Ca2$^+$ -ATPase. Proc Natl Acad Sci USA 87: 2466-2470, 1990 https://doi.org/10.1073/pnas.87.7.2466
  25. Tsuboi R, Sato C, Shi CM, Nakamura T, Sakurai T, Ogawa H. Endothelin-1 acts as an autocrine growth factor for normal human keratinocytes. J Cell Physiol 159: 213-220, 1994 https://doi.org/10.1002/jcp.1041590204
  26. Yada Y, Higuchi K, Imokawa G. Effects of endothelins on signal transduction and proliferation in human melanocytes. J Biol Chem 266: 18352-18357, 1991
  27. Yohn JJ, Morelli JG, Walchak SJ, Rundell KB, Norris DA, Zamora MR. Cultured human keratinocytes synthesize and secrete endothelin-1. J Invest Dermatol 100: 23-26, 1993 https://doi.org/10.1111/1523-1747.ep12349932