Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Interaction of Norfloxacin with Super-Coiled DNA

  • Hwangbo, Hyun-Jung (Department of Chemistry, Yeungnam University) ;
  • Lee, Young-Ae (Department of Chemistry, Yeungnam University) ;
  • Park, Jung-Hag (Department of Chemistry, Yeungnam University) ;
  • Lee, Yong-Rok (School of Chemical Engneering and Technology, Yeungnam University) ;
  • Kim, Jong-Moon (Division of Life and Molecular Sciences, Pohang University of Science and Technology) ;
  • Yi, Seh-Yoon (Applied Chemistry Major, Dongduk Women's University) ;
  • Kim, Seog K. (Department of Chemistry, Yeungnam University)
  • Published : 2003.05.20
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Abstract

Norfloxacin, that inhibits the action of topoisomerase Ⅱ, binds to wide variety of DNA. The binding mode of this drug to double- and super-coiled DNA (ds- and scDNA) is compared in this study by various spectroscopic methods, including absorption, fluorescence, and circular dichroism(CD) spectroscopy. Hypochromism in the absorption band, negative and positive induced CD bands (respectively in 240-260 nm and 270-300 nm region) are apparent for the norfloxacin that bound to both the dsDNA and scDNA. A decrease in fluorescence is also noticed in the presence of both DNAs. Since the spectroscopic characteristics are the same for both complexes, it is imperative that the binding mode of the norfloxacin is similar in ds- and scDNA. In the presence of $Mg^{2+}$, which is a cofactor in the topoisomerase Ⅱ action, the fluorescence intensity of the scDNA-norfloxacin complex increased and the resulting fluorescence intensity and shape was identical to that in the absence of scDNA. Therefore, the addition of an excess amount of $Mg^{2+}$ may result in the extrusion of norfloxacin from scDNA.

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