대한두경부종양학회지 (Korean Journal of Head & Neck Oncology)

  • 제18권2호
  • /
  • Pages.135-141
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  • 2002
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  • 1229-5183(pISSN)
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  • 2586-2553(eISSN)
대한두경부종양학회 (The Korean Society for Head and Neck Oncology)
권호 표지

후두암 세포주에서 $TGF-{\beta}1$에 의한 MMP2와 MMP9의 발현 양상

The Effect of Transforming Growth Factor-${\beta}1$ on Expression of MMP 2 and MMP 9 Cell Lines

  • 권남영 (고려대학교 의과대학 이비인후-두경부외과학교실) ;
  • 김형진 (고려대학교 의과대학 이비인후-두경부외과학교실) ;
  • 우정수 (고려대학교 의과대학 이비인후-두경부외과학교실) ;
  • 권순영 (고려대학교 의과대학 이비인후-두경부외과학교실) ;
  • 정광윤 (고려대학교 의과대학 이비인후-두경부외과학교실)
  • Kwon Nam-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Kim Hyung-Jin (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Woo Jeong-Su (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Kwon Soon-Young (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine) ;
  • Jung Kwang-Yoon (Department of Otolaryngology-Head and Neck Surgery, Korea University, College of Medicine)
  • 발행 : 2002.11.01
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초록

Backgrounds and Objectives: Metastasis is a complex multistep process that requires sequential interactions between the invasive cell and the extra-cellular matrix. Transforming growth factor-${\beta}1$ ($TGF-{\beta}1$) is a multifunctional regulator of cellular differentiation, motility and growth. Loss of sensitivity to the growth inhibitory effects by $TGF-{\beta}1$ plays important roles in neoplastic progression. The aim of this study was to investigate the role of $TGF-{\beta}1$ in the neoplastic invasion and metastasis through matrix metalloproteinase (MMP) of laryngeal cancer cell lines. Material and Methods: Two laryngeal cancer cell lines, SNU-899 and SNU-1076 were treated with recombinant $TGF-{\beta}1$, and the expression of MMP-2 and MMP-9 was immunohistochemically evaluated and gelatinase activity was studied by gelatin zymogram. Results: The cell growth inhibition was evident on 4th days after 1ng/ml and 10ng/ml $TGF-{\beta}1$ treatment. The expressions of MMP-2 and MMP-9, and their gelatinase activities were increased in dose-dependent manner. Conclusion: $TGF-{\beta}1$ treatment in laryngeal cancer cell lines induces the expression of MMP-2 and MMP-9, thus playing a role in the digestion of extracellular matrix gelatin.

키워드

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