IMMUNE NETWORK
- Volume 2 Issue 4
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- Pages.217-222
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- 2002
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
The Differential Staging of Murine Thymic Lymphoma Cell Lines, Scid.adh, R1.1 and EL-4
- Chae, Jong Seok (Department of Pathology, Kangwon National University College of Medicine) ;
- Kim, Hae-jung (Department of Pathology, Kangwon National University College of Medicine) ;
- Park, Weon Seo (Department of Pathology, Kangwon National University College of Medicine) ;
- Bae, Youngmee (Department of Pathology, Kangwon National University College of Medicine) ;
- Jung, Kyeong Cheon (Department of Pathology, Hallym University College of Medicine)
- Published : 2002.12.31
Abstract
Background: Scid.adh is a recently developed murine thymic lymphoma cell line, which has been used as in vitro model for the study of double negative stage III thymocytes. In this study, we compared the expression profile of a number of genes and proteins, which are tightly related to T cell development and apoptosis, in thymic lymphoma cell lines, R1.1, EL-4, and Scid.adh for the developmental staging. Methods: We examined the expression of development marker genes and proteins in three lymphoma cell lines by flow cytometry and RT-PCR. In addition, the expression of apoptosis-related molecules including bcl-2, bax and Fas was also investigated. Results: As previously reported, Scid.adh cell line expressed CD8 and CD25 but not TCR