백서의 비장에서 화학적 교감신경절제가 뜸(구(灸))자극으로 유도된 면역변조에 미치는 영향

The Effect of Chemical Sympathectomy on Moxibustion-Induced Immunomodulation in the Rat Spleen

  • 한재복 (경희대학교 대학원 동서의학과) ;
  • 오상덕 (경희대학교 대학원 동서의학과) ;
  • 이기석 (경희대학교 대학원 동서의학과) ;
  • 최기순 (경희대학교 대학원 동서의학과) ;
  • 조영욱 (경희대학교 의과대학 생리학교실) ;
  • 안현종 (경희대학교 의과대학 미생물학교실) ;
  • 배현수 (경희대학교 한의과대학 생리학교실) ;
  • 민병일 (경희대학교 대학원 동서의학과)
  • Han, Jae-Bok (Department of East-West Medicine, Graduate School, Kyung-Hee University) ;
  • Oh, Sang-Duck (Department of East-West Medicine, Graduate School, Kyung-Hee University) ;
  • Lee, Ki-Seok (Department of East-West Medicine, Graduate School, Kyung-Hee University) ;
  • Choi, Ki-Soon (Department of East-West Medicine, Graduate School, Kyung-Hee University) ;
  • Cho, Young-Wuk (Department of Physiology, Medical College, Kyung-Hee University) ;
  • Ahn, Hyun-Jong (Department of Microbiology, Medical College, Kyung-Hee University) ;
  • Bae, Hyun-Soo (Department of Physiology, Oriental Medical College, Kyung-Hee University) ;
  • Min, Byung-Il (Department of East-West Medicine, Graduate School, Kyung-Hee University)
  • 발행 : 2002.06.30

초록

Background: To investigate the role of sympathetic nervous system (SNS) in moxibustion-induced immunomodulation, the effects of chemical sympathectomy on moxibustion-induced changes in splenic NK cell cytotoxicity, T and B cell proliferation were studied in Sprague-Dawley male rats. Methods: Chemical sympathectomy was achieved with intraperitoneal injection of 6-hydroxydopamine 50 mg/kg/day for 3 successive days. Direct moxibustion (6-minute interval, 9 moxa ball, each of which weighing 0.007 g and burning for 40 seconds) was applied on unilateral anterior tibial muscle region where Zusanli (ST36) acupoint is located, once a day for 7 successive days. NK cell cytotoxicity was measured by $4hr-^{51}Cr$ release assay. Mitogen-induced lymphocyte proliferation was analyzed by [$^3H$]-thymidine incorporation assay. Results: NK cell cytotoxicity was suppressed by moxibustion, more in sympathectomized rats than in vehicle-treated rats. T cell proliferation induced by concanavalin A was not affected by moxibustion. B cell proliferation induced by lipopolysaccharide showed no significant change in vehicle-treated rats, but an increase in sympathectomized rats by moxibustion. Sympathectomy alone induced augmentation of NK cell cytotoxicity and suppression of T cell proliferation. Conclusion: These results suggest that SNS has no direct relation with moxibution-induced immunomodulation but has an important role in the mechanism to keep the homeostasis of immune system by tonically inhibiting excessive changes of various immune components.

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