Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effect of Bee Venom & melittin Herbal acupuncture solution to Apoptosis in NG pretreated neuroblastoma cell line

Bee Venom 및 Melittin 약침액(藥鍼液)이 신경마비물질(神經麻痺物質)을 처치(處置)한 신경아세포종(神經芽細胞腫)의 세포사(細胞死)에 미치는 영향(影響)

  • Lee, Jeong-Hoon (Department of Acupunture & Moxibustion College of Oriental Medicine, Kyung-San University) ;
  • Jin, Jae-do (Department of Acupunture & Moxibustion College of Oriental Medicine, Kyung-San University) ;
  • Lee, Seung-woo (Department of Acupunture & Moxibustion College of Oriental Medicine, Kyung-San University) ;
  • Han, Sang-Won (Department of Acupunture & Moxibustion College of Oriental Medicine, Kyung-San University) ;
  • Kim, Sang-Tae (Ja-Seng Research Institute of Biotechnology) ;
  • Shin, Jun-Shik (Ja-Seng Research Institute of Biotechnology)
  • 이정훈 (경산대학교 한의과대학 침구학교실) ;
  • 진재도 (경산대학교 한의과대학 침구학교실) ;
  • 이승우 (경산대학교 한의과대학 침구학교실) ;
  • 한상원 (경산대학교 한의과대학 침구학교실) ;
  • 김상태 (자생생명공학연구소) ;
  • 신준식 (자생생명공학연구소)
  • Received : 2001.11.03
  • Accepted : 2002.01.08
  • Published : 2002.02.20
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Abstract

Objective : This study is designed to investigate the effects of bee venom and melittin on cell death in neuroblastoma cell line after pretreatment with NG(nerve growth inhibitory substance) Methods : It was evaluated by using MTT assay, morphological method, DNA fragmenation, flow cytometry, immunocytochemistry analysis, RT-PCR and Western blot. Results : The MTT assay demonstrated that neuroblastoma cell viability was significantly inhibited dose-dependently by treatment with bee venom and melittin after pretreatment with NG in comparison awith control. The morphological study and fow cytometry demonstrated that neuroblastoma cell showed apoptosis. DNA fragmenation showed DNA ladder below 1 Kbp. Immunocytochemistry assay demonstrated that Fos and MAPK were down-regulated. RT-PCR analysis demonstrated that Fos and MAPK was down-regulated. Western blot demonstrated that Fos and MAPK were down-regulated from $1{\mu}g/ml$ bee venom in neuroblastoma cell pretreated with NG. Conclusion : These result suggests that bee venom and melittin after NG treatment have significant anti-cancer effect and further study is needed in vivo.

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