Journal of Acupuncture Research
- 제19권5호
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- Pages.57-72
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- 2002
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
봉약침액(蜂藥鍼液)이 세포활성(細胞活性)에 미치는 영향(影響)
The Effects of Bee Venom for Aqua-acupuncture on Cell viability
- 이승훈 (경산대학교 한의과대학 침구경혈학교실) ;
- 이봉효 (경산대학교 한의과대학 침구경혈학교실) ;
- 이경민 (경산대학교 한의과대학 침구경혈학교실) ;
- 조현열 (경산대학교 한의과대학 침구경혈학교실) ;
- 김영욱 (경산대학교 한의과대학 침구경혈학교실) ;
- 방재선 (경산대학교 한의과대학 침구경혈학교실) ;
- 서정철 (경산대학교 한의과대학 침구경혈학교실) ;
- 한상원 (경산대학교 한의과대학 침구경혈학교실)
- Lee, Seung-hoon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Lee, Bong-hyo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Lee, Kyung-min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Cho, Hyun-yeul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Kim, Youn-wook (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Bang, Jae-sun (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
- Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University)
- 투고 : 2002.07.13
- 심사 : 2002.09.14
- 발행 : 2002.10.20
초록
Objectives : This study was undertaken to determine the cytotoxic effects of crude bee venom which is widely used for aqua-acupuncture in oriental medical clinic. Methods : We compared the effects of crude bee venom, apamin, melittin and MCD peptide on cellviability by MTT asssay. Results : The obtained results are summarized as follows: 1. Bee venom, apamin, melittin and MCD peptide showed concentration--dependent cytotoxic effect in some human cell lines(human glioma cell line과 neuroblastorna, human mast cell line) for 24 and 48 Hour treatment. 2. Bee venom, apamin, melittin and MCD peptide showed dose-dependent cytotoxic effect in some human cell lines for 24 and 48 Hour treatment. 3. Bee venom treatment for 24 and 48 hour showed higher cytotoxic effects than apamin, melittin and MCD peptide. Conclusions : These results suggest that bee venom, apamin, melittin and MCD peptide have concentration- and dose- dependent cytotoxic effect in some human cell lines. But further study is needed for optimal concentration and dose.