Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Electron Microscopical Description on the Egg Stalk-like Structure of the Rockfish, Sebastes inermis (Teleostei: Scorpaenidae)

볼락 (Sebastes inermis) 난병 유사구조의 전자현미경적 기재

  • Lee Jung Sick (Department of Aqualife Medicine, Yosu National University) ;
  • Chang Young Jin (Department of Aquaculture, Pukyong National University)
  • Published : 2002.03.01
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Abstract

The ovary of the rockfish, Sebastes inermis was cystovarian condition and consists of numerous ovarian lobules. Previtellogenic oocytes were embedded in the connective tissue of the ovarian lobules. As the oocytes grows, it protruded into the ovarian cavity and form grapes cluster-like structure with egg stalk-like structure. Yolk granules and zona radiata were not observed in the embedded early oocytes about 20 $\mu$m in diameter. In the oocytes about 80 $\mu$m in diameter, yolk granules with electron dense were abundant in the ooplasm near the egg stalk-like structure. However, pinocytosis was not obsened in the pore canal system of the zona radiata. In this stage, microfilaments, developed endoplasmic reticula, tubular mitochondria, granular materials with low electron density and active multivesicular bodies were observed in the egg stalk-like structure. This results suggest that the egg stalk-like structure of the rockfish are related with the oocyte attachment and exogenous vitellogenesis of the intial vitellogenic oocyte.

볼락의 난소는 낭상형이며, 다수의 난소소엽으로 구성되어 있었다. 난황축적 이전의 난모세포들은 난소소엽의 결체성 조직에 매몰되어 관찰되었다. 난모세포는 성장하면서 난소강으로 돌출되어 난병 유사구조를 가지고 포도송이와 같은 구조를 나타냈다 난소 소엽내에 매몰되어있는 직경 약 20$\mu$m 크기의 초기 난모세포에서 난황과립과 방사대는 관찰되지 않았다 직경 약 80$\mu$m의 초기 난모세포의 난병 유사구조 세포질에서는 전자밀도가 높은 난황과려들이 다수 관찰되었으나, 방사대의 pore canal system에서 음세포작용은 관찰되지 않았다. 이 시기에 난병 유사구조에서는 미세섬유, 발달된 소포체와 미토콘드리아, 전자밀도가 낮은 과립상 물질 그리고 활성화된 multivesicular body들이 관찰되었다. 이러한 결과들은 볼락의 난병 유사구조는 난모세포의 부착과 초기 난모세포의 외재적 난황축적에 관여함을 시사하는 것이라고 할 수 있다.

Keywords

References

  1. Beams, H.W. and S.S. Sekhon. 1966. Electron microscope studies on the oocyte of the fresh-water mussel (Anodonta), with special reference to the stalk and mechanism of yolk deposition. J. Morphol., 119, 477-502 https://doi.org/10.1002/jmor.1051190407
  2. Chang, Y.J. and T.Y. Lee. 1982. Gametogenesis and reproductive cycle of the cockle, Fulvia mutica (Reeve). Bull. Korean Fish. Soc., 15, 241-253
  3. Chung, E.Y. and T.Y. Lee. 1985. Studies on the reproductive cycle of greenling, Agrammus agrammus. Bull. Nat'l Fish. Univ. Pusan, 25, 26-42 (in Korean)
  4. Elorduy-Garay, J.F. and S. Pamirez-Luna. 1994. Gonadal development and spawning of female ocean whitefish, Caulolatilus princeps (Pisces: Branchiostegidae) in the Bay of La Paz, B.C.S., Mexico. J. Fish Biol., 44, 553-566 https://doi.org/10.1111/j.1095-8649.1994.tb01233.x
  5. Hoar, W.S. 1957. The gonads and reproduction. In The Physiology of Fishes Vol. I, M.E. Brown, ed. Academic Press, New York, pp.287-321
  6. Lee, J.S., C.M. An and S.H. Huh. 1998. Reproductive cycle of goldeye rocklish, Sebastes thompsoni (Teleostei: Scorpaenidae). J. Korean Fish. Soc., 31, 8-16 (in Korean)
  7. Lee, J.S., J.C. Kang and S.H. Huh. 1997. Gonad structure and reproductive cycle of the smallmouth scorpionfish, Scorpaena miostoma (Teleostei: Scorpaenidae). J. Korean Fish. Soc., 30, 627-633 (in Korean)
  8. Lee, J.S., S.Y. Kim, K.H. Ma and S.H. Huh. 2000. Ovahan development and reproductive cycle of the female red tongue sole, Cynoglossus joyneri (Teleostei: Cynoglossidae). J. Korean Fish. Soc., 33, 554-558 (in Korean)
  9. Lee, T.Y. 1974. Gametogenesis and reproductive cycle of abalones. Publ. Mar. Lab. Busan Fish. Coll., 7, 21-50 (in Korean)
  10. Lee,T.Y. and S.Y. Kim. 1992. Reproduction and embryonic develop ment within the maternal body of ovoviviparous teleost, Sebastes inermis. Bull. Korean Fish. Soc., 25, 413-431 (in Korean)
  11. Nomura, M. 1962. Studies on reproduction of rainbow trout, Salmo eairdneri, with special reference to egg taking-I. Morphological changes of gonad in the process of maturation and the ovulation of eggs. Bull. Jap. Soc. Fish., 28, 409-415 https://doi.org/10.2331/suisan.28.409
  12. Riehl, R. 1978. Light and electron microscopical investigations on the oocytes of the freshwater teleost fishes Noemacheillus barbatulus L. and Gobio gobio L. (Pisces, Teleostei). Zool. Anz. Jena, 201, 199-219
  13. Selman, K. and R.A. Wallace. 1982a. The inter-and intracellular passage of proteins through the ovarian follicle in teleosts. In Proceedine of the International Symposium on Reproductive Physiology of Fish, C.J.J. Richter and H.J.T. Goos, eds. Pudoc, Wageninngen, pp. 151-154
  14. Selman, K. and R.A. Wallace. 1982b. Oocyte growth in the sheep shead minnow: Uptake of exogenous proteins by vitellogenic oocytes. Tissue and Cell, 14, 555-571 https://doi.org/10.1016/0040-8166(82)90047-7
  15. Selman, K. and R.A. Wallace. 1983. Oogenesis in Fundulus heteroclitus III. Vitellogenesis. J. Exp. Zool., 226, 441-457 https://doi.org/10.1002/jez.1402260315
  16. Selman, K. and R.A. Wallace. 1986. Gametogenesis in Fuadulus heteroclitus. Amer. Zool., 26, 173-192 https://doi.org/10.1093/icb/26.1.173
  17. Wallace, R.A. and K. Selman. 1981. Cellular and dynamic aspects of oocyte growth in teleosts. Amer. Zool., 21, 325-343 https://doi.org/10.1093/icb/21.2.325
  18. Yeon, I.J., S.H. Hong, Y.C. Park, J.S. Lee, S.T. Kim and H.K. Cha. 1997. The reproduction of Raja pulchra Liu in the Yellow Sea. Bull. Nat'l Fish. Res. Dev. Institute, 53, 23-36