Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The Effect of High Molecular Hyaluronic Acid on Bone Formation in Human Fetal Osteoblasts

사람 태아 골모세포에서 고분자 히알루론산의 골형성 유도에 관한 연구

  • Lee, Kwang-Soo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Hyun-A (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Yun-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 이광수 (원광대학교 치과대학 치주과학교실) ;
  • 김현아 (원광대학교 치과대학 치주과학교실) ;
  • 김윤상 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실)
  • Published : 2002.09.30
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Abstract

Hyaluronic acid (HA)는 중요한 glycosaminoglycan 중 하나로서 단백질과 화학적 결합을 하지 않기 때문에 분리가 쉽고 결합조직의 세포간 기질의 주요 성분이다. 우리는 점탄성 고분자 hyaluronic acid를 실험실상에서 사람 태아 골모세포의 골 형성 과정에 미치는 영향을 알아보고자 하였다. 우리는 여러 농도의 HA에 대한 사람 태아 골모세포에서의 세포증식, 염기성 인산분해효소 활성, 석회화 결절 형성능, 교원질 합성능 그리고 bone sialoprotein (BSP)의 발현 정도를 검사하였다. 세포증식에서 각 농도의 HA 처리군과 대조군 간에 2일과 4일간의 결과에서 유의한 차이를 보이지 않았다. 염기성 인산분해효소 활성에서는 0.063% HA 처리군에서 음성 대조군에 비해 가장 유의한 염기성 인산분해효소 활성을 보였다 (p<0.05). 0.063% HA 처리군은 교원질 합성능에서도 가장 높은 수준을 보였다 (p<0.05). 석회화 결절 형성능에서는 0.063% HA 처리군에서 대조군에 비해 많은 염색된 석회화 결절을 보였다. BSP의 발현 정도를 분석한 Western blot에서는 대조군에 비해 0.063% HA 처리군에서 증가된 단백질 발현을 나타났다. 본 연구 결과 고분자 HA가 실험실상에서 사람 태아 골모세포의 분화를 통해 새로운 골 형성을 유도할 수 있는 능력이 있음을 시사하였다.

Keywords

References

  1. Amsel, S., Maniatis, A., Tavassoli, M., and Crosby, W.H. : The significance of intramedullary bone formation in the repair of bone marrow tissue. Am J Anat 164: 101-112, 1969
  2. Anderson, H.C. : Mechanism of mineral formation in bone. Lab Invest 60: 320-330, 1989
  3. Arceo, N., Sauk, J,J., moehring, J., Foster, R.A., and Somerman, M,J. : Human periodontal cells initiate mineral - like nodules in vitro. J Periodontol 62: 499-503, 1991 https://doi.org/10.1902/jop.1991.62.8.499
  4. Bab, I., Gazit, D., Muhlrad, A., and Shteyer, A. : Renerating bone marrow produces a potent growth-promoting activity to osteogenic cells. Endocrinology 123: 345-352, 1988 https://doi.org/10.1210/endo-123-1-345
  5. Balazs E,A. and Gibbs D.A., 'The Rheoligical Properties and Biological Function of Hyaluronic Acid' in 'Chemistry and Molecular Biology of the Intercellular Matrix', volume 3, E. A. Balazs, ed., p.1241, Academic Press, N.Y, 1970
  6. Becker, W., Becker, B., Berg, L., Prichard, J., Caffessee, R, and Rosenberg, E.: New attachment after treatment with root isolation procedures : Report for treated class II and class II furcation and vertical osseous defects. Int J Perio Rest Dent 8(3): 2-16, 1988
  7. Beertsen, W. and Theo Van Den Bos, : Calcification of dentinal collagen by cultured rabbit periosteum : The role of alkaline phosphatase. Matrix 9: 159-171, 1989 https://doi.org/10.1016/S0934-8832(89)80035-6
  8. Bellow, C.G., Aubin, J.E., Heersche, J.N.M., and Antosz, M,E, : Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif Tissue Int 38: 143-154, 1986 https://doi.org/10.1007/BF02556874
  9. Bellows, C.G., Aubin, J.E., and Heershe, J.N.M. : Initiation and progression of mineralization of bone nodules formed in vitro : the role of alkaline Phosphatase and organic phosphate. Bone and mineral 14: 27-40, 1991 https://doi.org/10.1016/0169-6009(91)90100-E
  10. Bowers, G.M., Ganet, M., Stevens, M., Emerson, J., Corio, R, Mellonig,J., Lewis, S.B., Peltzman, B., Romberg, E., and Risdom, L. : Histological evaluation of new attachment in humans. J Priodontol 56: 381-396, 1985 https://doi.org/10.1902/jop.1985.56.7.381
  11. Branemark, P.I., Breine, U., Johansson, B., Royalance, P,J., Rockert, H., and Yoffey, J.M, : Regeneration of bone marrow: A clinical and experimental study following removal of bone Nmarrow by curettage. Acta Anat 59: 1-46, 1964 https://doi.org/10.1159/000142601
  12. Breen. M., Weinstein, H,G., and Johnson, R.T. : Acidic glycosaminoglycans in human skin during fetal development and adult life. Biochem Biophys Acta 201: 54-60, 1970 https://doi.org/10.1016/0304-4165(70)90009-7
  13. Brown, G.L., Curtsinger, L.J., White, M., Michell, R. O., Pietsch, J., Nordquist, R, and Fraunhofer, A.; Schultz, G.S. : Acceleration of tensile strengthof incisions treated with EGF and TGF-$\beta$ Ann Surg 208: 788-794, 1988 https://doi.org/10.1097/00000658-198812000-00019
  14. Butler, W.T. : Sialoproteins of bone and dentin, J. BioI. Buccale 19: 83-89, 1991
  15. Chen, J., Shapiro, H,S., and Sodek, J. : Developmental expression of bone sialoprotein mRNA in rat mineralized connective tissues. J Bone Min Res 7: 987-997, 1992 https://doi.org/10.1002/jbmr.5650070816
  16. De Bernard, B. : Glycoproteins in the local mechanism of calcification. Clin Orthop 162: 233-244, 1982
  17. Eyre D.R. : Collagentmolecular diversity in the body's protein scaffold. Science 207: 1315-1322, 1980 https://doi.org/10.1126/science.7355290
  18. Fisher, L.W., McBride, O,W., Termine, J.D., and Young, M,F.: Human bone sialoprotein : Deduced protein sequence and chromosomal Iocalization J BioI Chem 265: 2347-2351, 1990
  19. Flores, M.E., Norgard, M., Heinegard, D., Reinholt, F.P., and Andersson, G.: RGDDirected attachment of isolated rat osteoclasts to osteopontin, bone sialoprotein, and fibronectin, Short note. Exp Cell Res 201: 526-530, 1992 https://doi.org/10.1016/0014-4827(92)90305-R
  20. Furusawa, T. : The repaor process of intramedullary tissue after ablation of the tibial cavity of rat. Tokyo Jikeikai Med J 108: 591-607, 1993
  21. Hata R, Hori H., Nagai Y., Tanaka S., Kondo M., Kiramatsu M., Utsumi N., and Kumegawa M. ; Selective inhibition of type I collagen synthesis in osteoblastic cells by epidermal growth factor, Endocrinology 115: 867-876, 1984 https://doi.org/10.1210/endo-115-3-867
  22. Hunter, G.K., and Goldberg, H.A.: Nucleation of hydroxyapatite by bone sialoprotein. Proc, Natl Acad Sci USA 90: 8562-8565, 1993 https://doi.org/10.1073/pnas.90.18.8562
  23. Hunter, G.K., and Goldberg, H.A. : Modulation of crystal formation by bone sialoprotein: role of glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein, Biochem J 302: 175-179, 1994 https://doi.org/10.1042/bj3020175
  24. Kasugai, S., Nagata, T., and Sodek, J. : Temporal studies on the tissue compartmentalization of bone sialoprotein (BSP), osteopontin (OPN), and SPARC protein during bone formation in vitro. J Cell Physiol 152: 467-477, 1992 https://doi.org/10.1002/jcp.1041520305
  25. Kim, H.D. and Valentini RF. : Retention and activity of BMP-2 in hyaluronic acid-based scaffolds in vitro, J Biomed Mater Res 59: 573-584, 2002 https://doi.org/10.1002/jbm.10011
  26. Kvist, T.N. and Finnegan, C.V. : The distribution of glycosaminoglycans in the axial region of the developing chick embryo. I. Histochemical Analysis J Exp Zool 175: 221-240, 1970a
  27. Kvist, T.N. and Finnegan, C.V. : The distribution of glycosaminoglycans in the axial region of the developing chick embryo. II. Biochemical analysis J Exp Zool 175, 221-240, 1970b
  28. Loewi, G. and Meyer, K. : The acid mucopolysacchride of embryonic skin Biochemi Biophys Acta 27: 453-456, 1958 https://doi.org/10.1016/0006-3002(58)90371-8
  29. McConnachie, P. R. and Ford, P, : Acid mucopolysacchrides in the development of the Pacific great skate, Raja binoculata. J Ernbryol Exp Morphol 16: 17-28, 1966
  30. McKee, M.D. and Nanci, A,: Postembedding colloidal-gold immunocytochemistry of noncollagenous extracellular matrix proteins in mineralized tissues. Microsc Res Tech 31: 44-62, 1995 https://doi.org/10.1002/jemt.1070310105
  31. Mizuno M., Kitajima T., Tomita M., and Kuboki Y. : The osteoblastic MC3T3-El cells synthesize C-terminal propeptide of type I collagen, which promoted cell-attachment of osteoblasts, Biochim Biophys Acta 1310: 97-102, 1996 https://doi.org/10.1016/0167-4889(95)00151-4
  32. Mustoe, T.A., Piece, G.F., Thomason, A., Gramates, P., Sporn, M.B., and Deuel, T.F. : Accerated healing of incisional wound in rats induced by transforming growth factor-$\beta$. Science 237: 1333-1336, 1987 https://doi.org/10.1126/science.2442813
  33. Nagata, T., Bellows, C,G., Kasugai, S., Butler, W.T., and Sodek, J.:Biosynthesis of bone proteins 'SPP - I (secreted phosphoprotein-I, osteopontin), BSP (bone sialoprotein) and SPARC (osteonectin)' in association with mineralized tissue formation by fetal rat-calcarial cells in culture. J Biochem 274: 513-520, 1991 https://doi.org/10.1042/bj2740513
  34. Oldberg, A., Franzen, A., and Heinegard, D., Pierschbacher, M. and Ruislahti, E. : The primary structure of bone sialoprotein. J BioI Chem 263: 19433-19436, 1988
  35. Owen, T,A., Aronow, M., Shalhoub, V., Barone, L.M., Wilming, L., Tassiinari, M.S., Kennedy, M.B, Pockwinse, S., Lian, J.B., and Stein, G.S. : Progressive development of the rat osteoblast in vitro : Reciprocal relationships in expression of genes associated with osteoblast proliferation and differantiation during formation of the bone extracellular matrix. J Cell Physiol 143: 420-430 1990 https://doi.org/10.1002/jcp.1041430304
  36. Patt, H.M. and Maloney, M.A. : Bone marrow regeneration after local injury: A review. Exp Haematol S: 135-146, 1975
  37. Pontoriero, R., Lindhe, J., Nyman, S., Karring, T., Rosenberg, E., and Sanavi, F. : Guided tissue regeneration in degree II furcation involved mandibular molars. J Clin Periodontol 15: 247-254, 1988 https://doi.org/10.1111/j.1600-051X.1988.tb01578.x
  38. Rojkind M. : Chemistry and biosynthesis of collagen. A review. Bull Rheum Dis 30(1): 1006-1010, 1979-80
  39. Schanllhorn, R. and McClain, P. : Combined osseous composite grafting, root conditioning,and guided tissue regeneration. Int J Perio Rest Dent 8: 9-32, 1988
  40. Shapiro, H.S., Chen, J., Wrana, J.L., Zhang, Q., Blum, M., and Sodek, J.: Characterization of porcine bone sialoprotein: primary structure and cellular expression. Matrix 13: 431-440, 1993 https://doi.org/10.1016/S0934-8832(11)80109-5
  41. Sodek, J., Chen, J., Kasugai, S., Nagata, T., Zhang, Q., McKee, M.D., and Nanci, A.: Elucidating the functions of bone sialoprotein and osteopontin in bone formation, pp. 297-306, In: H. Slavkin and P. Price (ed), Chemistry and Biology of Mineralized Tissues. B.V Elsevier Sciecne Publishers, 1992
  42. Soh, Y. S.: Hyaluronic Acid. Properties and Applications. Polyrnertkorea) 12(6): 484-488, 1988
  43. Tenenbaum, H. C. and Heersche, J. N. M. : Dexamethasone stimulates osteogenesis in chick periosteum in vitro. Endocrinology 117: 2211-2217, 1985 https://doi.org/10.1210/endo-117-5-2211
  44. Veit K, Beate B. S., and Franz W. : Hyaluronic acid influences the migaration of myoblasts within the avian embryonic wing bud. American J Anatomy 192: 400-406, 1991 https://doi.org/10.1002/aja.1001920407
  45. Watanabe, C., Yamada, H., Watanabe, Y. Kikuchi, E., Ito, H., Sonobe, H., and Sasaki, T. :A histological study of bone fonnayion stimulation in filling bone defects by fibrin adhesive material (Beriplast P). Jpn J Oral Maxillofac Surg 38: 353-365, 1992 https://doi.org/10.5794/jjoms.38.353
  46. Watson, J.M., Parrish, E.A. and Rinehart, C.A.: Selective potentiation of gynecologic cancer cell growth in vitro by electromagnetic fields. Gynecol Oncol 71: 64-71, 1998 https://doi.org/10.1006/gyno.1998.5114
  47. Weiss, L. : The hematopoietic microenvironment of the bone marrow: An ultrastructuaral study of the stroma in rats. Anat Rec 186: 161-184; 1976 https://doi.org/10.1002/ar.1091860204
  48. West, D. C. and Kumar, S. : Hyaluronan and angiogenesis. The biology of hyaluronan, Chiba Foundation symposium, 143. Chichester Wiley 187-207, 1989
  49. Yao, K.L., Todescan, R., and Sodek, J.: Temporal changes in matrix protein synthesis and mRNAexpression during mineralized tissue formation by adult rat bone marrow cells in culture. J Bone Min Res 9: 231-240, 1994 https://doi.org/10.1002/jbmr.5650090212
  50. Zhang, G.S., Tu, G.Q., Zhang, G.Y., Zhou, G.B., and Zheng, W.L.: Indomethacin induces apoptosis and inhibits proliferation in chronic myeloid leukemia cells. Leukemia Res 24: 385-392, 2000 https://doi.org/10.1016/S0145-2126(99)00198-8
  51. Zhumabayeva, B.D., Lin, W.L., Choung, P.H., Chien, H.H., Sodek, J., Sampath, KT., and Cho, M.I.: Differential induction of bone sialoprotein by dexamethasone and osteogenic protein-1 (OP-1, BMP-7) in rat periodontal ligament cells in vitro: relationship to the mineralization of tissue nodules, Int J Oral Bio 91-101, June 1988