Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Mutation Analysis in β2-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC)

DHPLC를 이용한 β2-교감신경수용체 유전자에서의 돌연변이 분석

  • Park, Sang-Bum (Dept. of Chemistry, College of Natural Science, Dankook University) ;
  • Oh, Chung-Hun (Medical Laser Research Center, Dankook University) ;
  • Kim, Jong-Wan (Dept. of Clinical Pathology, Dankook University Medical College) ;
  • Jang, Won-Cheoul (Dept. of Chemistry, College of Natural Science, Dankook University)
  • 박상범 (단국대학교 자연과학대학 화학과) ;
  • 오충훈 (단국대학교 의학레이저연구센터) ;
  • 김종완 (단국대학교 의과대학 임상병리과) ;
  • 장원철 (단국대학교 자연과학대학 화학과)
  • Received : 2002.01.18
  • Published : 2002.06.25
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Abstract

We analysed mutation of ${\beta}_2$-adrenergic receptor gene that controls bronchial asthma by denaturing high performance liquid chromatography (DHPLC) according to ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC). We extracted genomic DNA from 50 asthma patients, amplified DNA using PCR, and analysed PCR product by DHPLC. As a result, we obtained that mutation frequency was 15 (30%) among 50 cases. Consequently DHPLC mutation detection was confirmed that the result of direct sequencing was coincide exactly.

본 연구에서는 ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC)방식을 이용한 denaturing high performance liquid chromatography (DHPLC)방법을 사용하여 기관지 천식을 조절하는 ${\beta}_2$-교감신경수용체 유전자의 돌연변이를 검출하였다. 50명의 천식 환자의 혈액에서 genomic DNA를 추출하여 중합효소연쇄반응 (polymerase chain reaction, PCR)을 이용해 증폭하고, 그 산물을 DHPLC로 분석한 결과, 50개의 시료 가운데 15개 (30%)의 돌연변이를 검출하였다. 최종적으로 유전자 염기서열결정법을 통해 DHPLC의 돌연변이 검출률이 정확함을 확인하였다.

Keywords

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