Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Immuno-Electron Microscopic Studies on the Localization of Serotonin and Somatostatin in the Optic Lobes of Cephalopods (Todarodes pacificus and Octopus minor) Inhabiting the Korean Waters

한국 연근해산 두족류 (Todarodes pacificus and Octopus minor) 시엽 (Optic lobe)내 Serotonin 및 Somatostatin의 분포에 관한 면역전자현미경적 연구

  • Chang, Nam-Sub (Department of Life Science, College of Natural Science, Mokwon University) ;
  • Han, Jong-Min (Department of Life Science, College of Natural Science, Mokwon University) ;
  • Kim, Sang-Won (Department of Life Science, College of Natural Science, Mokwon University) ;
  • Lee, Kwang-Ju (Dept. of Anatomy, Chungbuk National University College of Medicine) ;
  • Hwang, Sun-Jong (Dept. of Anatomy, Chungbuk National University College of Medicine) ;
  • Lee, Jung-Chan (Dept. of Anatomic Pathol, Eulji Univ. Hospital)
  • 장남섭 (목원대학교 자연과학대학 생명과학부) ;
  • 한종민 (목원대학교 자연과학대학 생명과학부) ;
  • 김상원 (목원대학교 자연과학대학 생명과학부) ;
  • 이광주 (충북대학교 의과대학 해부병리과) ;
  • 황선종 (충북대학교 의과대학 해부병리과) ;
  • 이정찬 (을지의과대학병원 해부병리과)
  • Published : 2002.09.01
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Abstract

In this study, we carried out immunostaining and immunogold labeling with antibodies to serotonin and somatostatin to examine the characteristics and functions of the neurons that secrete neurotransmitters in optic lobes of Todarodes pacificus and Octopus minor. As a result of immunostaining with anti-somatostatin, the nerve cells of Todarodes pacificus reacted as similar to the anti-serotonin, but in Octopus minor, only large cells in the outer granule cell layer reacted positively. In the immunogold labeling with anti-serotonin, the nerve cells in the inner grande cell layer and medulla of Todarodes pacificus reacted strongly, 30 gold particles being labeled per $0.5{\mu}m^2$ of the cytoplasm. However, in Octopus minor, only 17 gold particles were labeled, which stated a weak reaction. On the other hand, in the anti-somatostatin case, the nerve cells in the outer and inner granule cell layers and medulla of Todarodes pacificus showed strong reaction, 30 gold particles being labeled per $0.5{\mu}m^2$ of the cytoplasm while the nerve cells in the outer granule cell layer of Octopus minor reacted weakly, about 3 gold particles being labeled per the equivalent area. As a result of immunostaining and immunogold labeling with two types of antibodies to each part of the optic lobes, we found that the reactive nerve cells were distributed differently in the two species. In particular, the degree of reactivity to the immunostaining and immunogold labeling appeared stronger in Todarodes pacificus than in Octopus minor.

두족류 Octopus minor와 Todarodes pacificus의 시엽내 신경전달물질을 분비하는 neuron의 특성 및 기능을 확인하기 위해 serotonin 및 somatostatin의 항체를 사용한 면역염색과 면역금표지법을 시행하였다. 면역염색 결과 항-somatostatin은 살오징어인 경우 항-serotonin 면역반응과 유사하게 나타났지만, 서해낙지에서는 외과립세포층의 큰 세포에서만 반응을 보였다. 항-serotonin을 이용한 면역금표지법은 살오징어인 경우 내과립세포층과 수질부의 신경세포에서는 세포질 $0.5{\mu}m^2$당 30개 정도의 금입자가 관찰되어 강한 반응을 보인 반면, 서해낙지에서는 17개 정도의 비교적 약한 반응을 보였다. 항-somatostatin에서는 살오징어의 외과립 및 내과립세포층 그리고 수질부의 반응된 세포의 세포질 $0.5{\mu}m^2$당 30개 정도의 금입자가 관찰되어 강한 반응을 보인 반면, 서해낙지의 외과립세포층의 세포에서는 3개 정도의 금입자만이 관찰되어 역시 약한 반응을 보였다. 이와 같이 시엽의 각 부위별 면역염색과 면역금표지법을 시행한 결과 2종류의 항체에 각각 양성반응을 보인 신경세포들이 두 종에서 다양하게 분포하고 있음을 확인했는데 특히 면역염색과 면역금표지법에 관한 반응외 정도를 비교하면 서해낙지에 비해 살오징어에서 비교적 강하게 나타났다.

Keywords

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