Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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The Effects of Sophorae radix Extracts on CGRP Immunoreactive Neurons of Spinal Cord and Ganglia in Experimental Arthritic Rat Model

실험적 관절염 흰쥐 모델에서 고삼추출액이 척수와 척수신경절의 CGRP 면역반응 신경원에 미치는 영향

  • Shin Hyun Jong (Departments of Oriental pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee Kwang Gyu (Departments of Oriental pathology, College of Oriental Medicine, Woosuk University) ;
  • Ryuk Sang Won (Departments of Classics, College of Oriental Medicine, Woosuk University) ;
  • Lee Sang Ryong (Departments of Meridianology, College of Oriental Medicine, Woosuk University) ;
  • Ko Byung Moon (Departments of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Lee Chang Hyun (Departments of Anatomy, College of Oriental Medicine, Woosuk University)
  • 신현종 (우석대학교 한의과대학 병리학교실) ;
  • 이광규 (우석대학교 한의과대학 병리학교실) ;
  • 육상원 (우석대학교 원전의사학교실) ;
  • 이상룡 (우석대학교 한의과대학 경혈학교실) ;
  • 고병문 (우석대학교 한의과대학 해부학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Published : 2002.02.01
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Abstract

To investigate the antiinflammatory and analgesic effects of Sophorae radix extracts administered to the arthritic rat model, immunohistochemical stains for CGRP in the L4, L5 and L6 spinal cord and ganglia were done, and paw swelling thickness were measured. Complete Freund,s Adjuvant(CFA) were injected to subcutaneous tissue of left foot paw of rats to induce arthritis. Sophorae radix extracts was administered immediately after CFA injection for 10 days. The spinal cord and ganglia were frozen sectioned(30㎛). These sections were stained by CGRP immunohistochemical staining method, and observed with light microscope. The results were as follows : 1. The change of paw swelling thickness of experimental group decreased from 4 day to 10day after CFA injection compared to control group. 2. The change of differential leukocytes counts of experimental group increased the ratio of lymphocytes. and decreased the ratio of neutrophils compared to control group. 3. The change of CGRP immunoreactive nerve fiber of dorsal horn of experimental group was dense stained compared to control group. 4. The number of CGRP immunoreactive neurons of L4 and L5 spinal cord of experimental group was less than in those control group. These results suggested that Sophorae radix extracts reduces the number of CGRP immunoreactive neurons and nerve fibers of spinal cord and ganglia, and decrease paw swelling thickness in arthritic rat model, which may be closely related to analgesic and antiinflammatory effects of Sophorae radix.

Keywords

References

  1. 全國中草藥匯編 全國中草藥匯寫組
  2. 대한동의생리병리학회지 v.15 동의보감의 질병분류에 대한 연구(3) 정우열
  3. 간추린 병리학 대한병리학회 대구·경북지부학회
  4. 석사학위논문 Adjuvant關節炎에 疎經活血湯合黃連解毒湯이 미치는 實驗的 硏究 金荀中
  5. 석사학위논문 羌活湯 및 加味羌活湯이 鎭痛效果와 Adjuvant 關節炎 白鼠에 미치는 영향 尹英姬
  6. 석사학위논문 行濕流氣散이 Adjuvant 關節炎에 미치는 實驗的 硏究 李世鉉
  7. 석사학위논문 鍼灸刺戟 및 疎風活血湯加味方 投與가 류마티스性 關節炎 患者의 血液에 미치는 影響 安仙濬
  8. 석사학위논문 生草烏와 法製草烏水鍼이 흰쥐의 Adjuvant 關節炎에 미치는 影響 盧富來
  9. 석사학위논문 獨活寄生湯加猫脛骨이 Adjuvant 關節炎에 미치는 實驗的 硏究 徐海卿
  10. Pain v.52 effects of morphine and naloxone on basal and evoked Fos-like immunoreactivity in lumbar spinal cord neurons of arthritic rats Abbadie, C.;Besson, J.M. https://doi.org/10.1016/0304-3959(93)90110-B
  11. Regul. Pept. v.51 Changes of spinal substance P, calcitonin gene-peptide, somatostatin, Met- enkephalin and neurotensin in rats in response to formalin induced pain Zhang, R.X.;Mi, Z.P.;Qiao, J.T. https://doi.org/10.1016/0167-0115(94)90131-7
  12. Substance P. Pharmacol. Rev. v.35 Pernow, B.
  13. Brain Res. v.168 Substance P depletion in the dorsal horn of rat spinal cord after section of the peripheral processes of primary sensory neurons. Jessel, T.M.;Tsungo, A.;Kanazawa, I.;Otsuka, M. https://doi.org/10.1016/0006-8993(79)90167-7
  14. Brain Res. v.114 Effects of substance P on functionary identified units in cat spinal cord. Henry, J.L. https://doi.org/10.1016/0006-8993(76)90965-3
  15. Exp. Neurol. v.99 A light and electron microscopic study of calcitonin gene-related peptide in the spinal cord of the rat McNeil, D.L;Coggeshal, R.E.;Carlton, S.M. https://doi.org/10.1016/0014-4886(88)90186-0
  16. 東醫再活醫學科學 全國韓醫科大學 再活醫學科學敎室
  17. 本草綱目 李時珍
  18. 東醫寶鑑 許浚
  19. 本草學 朱榮丞 外
  20. 現代本草 中國藥材學 戴新民
  21. 國譯編註方藥合編 黃度淵
  22. 原色臨床本草學 辛民敎
  23. 校正 中藥大辭典 醫聖堂編輯部
  24. 현대본초학 안덕균 외
  25. 中藥志(第一冊) 中國醫學科學院 藥物硏究所 等
  26. Pain v.28 Evidence that adjuvant arthritis in the rat is associated with chronic pain. Colpaert, F.C. https://doi.org/10.1016/0304-3959(87)90117-5
  27. Pain v.28 Dorsal horn (convergent) neurons in the intact anesthetized arthritic rat. 1. Segemntal excitatory influences. Calvine, B.;Villanueva, L.;Le Bars, D. https://doi.org/10.1016/0304-3959(87)91063-3
  28. Inflammation v.24 Carrageenan-induced arthritis in the rat Hansra, P.;Moran, E.L.;Fornasier, V.L.;Bogoch, E.R. https://doi.org/10.1023/A:1007033610430
  29. Int. J. Exp. Pathol. v.71 Mast cells in early stages of antigen-induced arthritis in rat knee joints. Tiggelman, A.M.;Van Noorden, C.J.
  30. Neurosci. Lett v.67 Somatostatin and calcitonin gene- related peptide synergistically modulate spinal sensory and reflex mechanism in the rat: behavioral and electrophysi -ological studies. Wiesenfeld-hallin, Z. https://doi.org/10.1016/0304-3940(86)90329-0
  31. Exp. Neurol. v.99 A light and electron microscopic study of calcitonin gene-related peptide in the spinal cord of the rat. McNeill, D.L.;Coggeshall, R.E.;Carlton, S.M. https://doi.org/10.1016/0014-4886(88)90186-0
  32. Peptide v.6 Calcitonin gene-related peptide coexist with substance P in capsaicin sensitive neurons and sensory ganglia of the rat. Skofitsch, G.;Jacobowitz, D.M. https://doi.org/10.1016/0196-9781(85)90179-2
  33. Pain v.54 Increased in vivo release of calcitonin gene related peptide-like material from the spinal cord in arthritic rats. Collin, E.;Mantelet S.;Frechilla, D.;Pohl, M.;Bourgoin, S.;Hamon, M.;Cesselin, F. https://doi.org/10.1016/0304-3959(93)90210-G
  34. Pain v.55 Behavioral and immunohistochemical changes in an experimental arthritis model in rats. Sluka, K.A.;Westlund, K.N. https://doi.org/10.1016/0304-3959(93)90013-F
  35. Rheumatol. Int. v.12 Studies on carrageenan-induced arthritis in adult rats: presence of nerve growth factor and role of sympathetic innervation. Aloe, L.;Tuveri, M.A.;Levi-Montalcini, R. https://doi.org/10.1007/BF00302155