Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Artemisia iwayomogi on the Airway Smooth Muscle

더위지기가 기관지평활근에 미치는 영향

  • Park Jin Young (Department of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Shim Jin Chan (Department of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Ho Keun (Department of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Sun Sung Gyu (Department of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Jin Sang Sik (Department of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Jong Chun (Department of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Han Jong Hyun (Department of Pharmacology, College of Oriental Medicine, Wonkwang University)
  • 박진영 (원광대학교 한의과대학 약리학교실) ;
  • 심진찬 (원광대학교 한의과대학 약리학교실) ;
  • 김호근 (원광대학교 한의과대학 약리학교실) ;
  • 선성규 (원광대학교 한의과대학 약리학교실) ;
  • 진상식 (원광대학교 한의과대학 약리학교실) ;
  • 김종천 (원광대학교 한의과대학 약리학교실) ;
  • 한종현 (원광대학교 한의과대학 약리학교실)
  • Published : 2002.04.01
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Abstract

Artemisia iwayomogi has been used in Korea for many centuries as a treatment for anemia. The effect of Artemisia iwayomogi on tracheal smooth muscle is not known. The purpose of the present study is to determine the effect of Artemisia iwayomogi on histamine induced tracheal smooth muscle contraction in guinea pigs. Guinea pigs(500g, male) were killed by CO₂ exposure and a segment (8-10mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted ‘in pairs’ in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine (His) which evoked 50% of maximal response (ED/sub 50/) was obtained from cumulative dose response curves for histamine (10/sup -7/～10/sup -4/M). Contractions evoked by His (ED/sub 50/) were inhibited significantly by Artemisia iwayomogi. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 31.9% (p<0.05) after 100㎕/㎖ Artemisia iwayomogi. L-NNA slightly but significantly attenuated the inhibitory effects of Artemisia iwayomogi. Following treatment with L-NNA, the mean percent inhibition caused by 100㎕/㎖ Artemisia iwayomogi fell to 44.6% in guinea pig induced by histamine contraction. Propranolol, indomethacin and methylene blue did not significantly alter the inhibitory effect of Artemisia iwayomogi. These results indicate that Artemisia iwayomogi can relax histamine induced contraction of guinea pig, and that this inhibition related to nitric oxide formation.

Keywords

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