Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Scorpio water extract on Cultured Spinal Sensory Neurons Damaged by Xanthine Oxidase/Hypoxanthine

전갈 전탕액이 XO/HX에 의해 손상된 배양 척수감각신경세포에 미치는 효과

  • Yang Heung Su (Department of Physiology, Collage of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, Collage of Oriental Medicine, Wonkwang University) ;
  • Song Yong Sun (Department of Oriental Rehabilitation Medicine, Collage of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, Collage of Oriental Medicine, Wonkwang University)
  • 양흥수 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 송용선 (원광대학교 한의과대학 재활의학과) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2002.06.01
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Abstract

To study the effects of Scorpio on oxygen free radical-mediated damage by xanthine oxidase/hypoxanthine (XO/HX) on cultured spinal sensory neurons, in vitro assays such as MTT assay were used in cultured spinal sensory neurons derived from mice. Spinal sensory neurons were cultured in media containing various concentrations of XO/HX for 6 hours, after which the neurotoxic effect of XO/HX was measured by in vitro assay. The protective effect of the herb extract, Scorpio water extract against XO/HX-induced neurotoxicity was also examined. The results are as follows : In MTT assay, XO/HX significantly decreased the cell viability of cultured mouse spinal sensory neurons according to exposure concentration and time in these cultures. The effect of Scorpio water extract on XO/HX-induced neurotoxicity showed a quantitative increase in neurdfilament. These results suggest that XO/HX has a neurotoxic effect on cultured spinal sensory neurons from mice and that the herb extract, Scorpio water extract, was very effective in protecting XO/HX-induced neurotoxicity.

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References

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