Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Relationships Between Bovine Growth Hormone Gene Polymorphism and Semen Characteristics in Hanwoo Bull

한우 종모우의 소 성장호르몬 유전자 다형과 정액성상과의 관계

  • Lee, S.S. (Livestock Improvement Center, NACF) ;
  • Kim, J.H. (Livestock Improvement Center, NACF) ;
  • Jeong, J. (Livestock Improvement Center, NACF) ;
  • Park, N.H. (Livestock Improvement Center, NACF)
  • 이성수 (농협중앙회 가축개량사업소) ;
  • 김진호 (농협중앙회 가축개량사업소) ;
  • 정준 (농협중앙회 가축개량사업소) ;
  • 박노형 (농협중앙회 가축개량사업소)
  • Published : 2002.12.31
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Abstract

The objects of this study were to estimate gene frequencies of the bovine growth hormone(bGH), and to investigate the relationship between the bGH polymorphism and semen characteristics in Hanwoo bull. One hundred nine heads of Hanwoo bulls were used to identify bGH genotypes by the PCR-RFLP, followed by digestion with Alu I restriction enzyme. The frequencies of leucine(Leu) and Valine(Val) alleles were 0.88 and 0.12, respectively. Observed number of LL, LV and VV genotypes were 83, 25 and 1, respectively. Semen characteristics(semen volume, sperm concentration) were analyzed by GH genotypes in 25,559 ejaculates of 109 heads. Although bGH genotypes showed no significant effects on semen characteristics, those of bulls with VV genotype were tended to be lower than those of other bulls with LL or LV genotypes. And, in 1998, total sperm number(60.47${\times}$$10^8$) of VV bulls were significantly lower(P<0.05) than those(86.21${\sim}$92.22${\times}$$10^8$) of other genotypes bulls. This results provide that the VV bull in bGH locus may be worse, under the LL and LV bulls on semen characteristics. However, the number of examined VV bulls was only one and further investigations are needed to confirm the results.

한우 종모우에 있어 소 성장호르몬 유전자의 출현빈도를 알아보고 소 성장호르몬 다형과 정액성상과의 관계를 살펴보기 위하여 실시하였다. 한우 종모우 109두의 소 성장호르몬 유전자를 Alu I 제한효소로 처리하여 PCR-RFLP로 분석하였다. Leucine(Leu)과 valine(Val) 유전자의 출현빈도는 각각 0.88과 0.12이었다. 소 성장호르몬 VV 유전자형을 지닌 한우 종모우가 다른 유전자형을 지닌 종모우보다 정액성상(정액량, 정자농도, 총정자수)이 떨어지는 경향을 보였지만 소 성장호르몬의 유전자형이 정액성상에 유의적인 영향은 미치지 못하였다. 채취순번에 따른 영향에 있어서도 VV 유전자형을 지닌 종모우가 다른 유전자형을 지닌 종모우보다 정액성상이 떨어지는 경향을 보였지만 유의적인 차이는 나타내지는 못하였으며 다만 년도에 따른 영향에 있어 1998년도 VV 유전자형을 지닌 종모우가 다른 유전자형을 지닌 종모우보다 총정자수에 있어 유의적으로 적게 나타났다(P<0.05). 전체적으로 소 성장호르몬 유전자의 VV 유전자형을 지닌 종모우의 정액성상이 다른 유전자를 지닌 종모우의 정액성상보다 낮은 경향을 보였지만, 조사한 109두의 한우 종모우 중 VV 유전자형을 지닌 종모우가 1두로 분석되어 이 종모우의 정액성상만을 이용하여 소 성장호르몬 유전자다형이 정액성상에 미치는 영향을 살펴보았기에 종모우 선발시 기초자료로 이용하기에는 추가적인 시험이 필요할 것으로 사료된다.

Keywords

References

  1. Almquist, J. O. 1973. Effects of sexual pre- parations on sperm output, semen characteristics and sexual activity of beef bulls with a comparison to dairy bulls. J. Anim. Sci. 36:331.
  2. Chikuni, K., Terada, F., Kageyama, S., Koishi- gawa, T., Kato, S. and Ozutsumi, K. 1991. Identification of DNA sequence variants for amino acid residue 127 of bovine growth hormone using the polymerase chain reaction method. Anim. Sci. Technol.(Jpn.). 62:660.
  3. Davis, S. L., Senger, P. L., Ohlson, D. L. and Hossner, K. L. 1985. Temporal patterns of growth hormone and seminal plasma of mature daily bulls. J. Anim. Sci. 60:35.
  4. Everett, R. E., Bean, B. and Foote, R. H. 1978. Sources of variation of semen output. J. Dairy Sci. 61:90.
  5. Everett, R. E. and Bean, B. 1982. Environmental influences on semen output. J. Dairy Sci. 65:1303.
  6. Flipse, R. J. and Almquist, J. O. 1961. Effect of total digestible nutrient intake to four years of age on growth and reproductive development and performance of dairy bull. J. Dairy Sci. 44:905.
  7. Glanders, H. J., Kratzsch, J., Weisbrich, C. H. and Birkenmeier, G. 1996. Insulin-like growth factor I in bovine seminal plasma correlate with semen quality. Human Reprod. 11:2454.
  8. Grochowska, R., Sorensen, P., Zwierzchowski, L., Snochowski, M. and Lovendahl, P. 2001. Genetic variation in stimulated GH release and in IGF-I of young dairy cattle and their associations with the leucine/valine polymorphism in the GH gene. J. Anim. Sci. 79:470.
  9. Hoj, S., Fredholm, M., Larsen, N. J. and Nielsen, V. H. 1993. Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle. Animal Genetics. 24:91.
  10. Henricks, D. M., Kouba, A. J., Lackey, B. R., Boone, W. R. and Gray, S. L. 1998. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa : influence on sperm motility. Biol. Reprod. 59:330.
  11. Lechniak, D., Machnik, G., Szydlowski, M. and Switonski, M. 1999. Growth hormone gene polymorphism and reproductive performance of AI bulls. Theriogenology. 52:1145.
  12. Lechniak, D., Machnik, G., Szydlowski, M. and Switonski, M. 1999. Relationship between growth hormone(GH) gene polymorphism and sperm characteristics in AI bulls. Theriogenology. 51:346.
  13. Lee, B., Crooker, B., Murtaugh, M., Hansen, L. and Chester-Jones, H. 1993. Association of somatotropin(bST) gene polymorphism with selec- tion for milk yield in Holstein cows. J. Dairy Sci. 76(Suppl 1):149.
  14. Lobie, P. E., Breipohl, W., Stragon, J. G. and Waters, M. J. 1990. Cellular location of the growth hormone receptor/binding protein in the rat male and female reproductive systems. Endocrinology. 126:2214.
  15. Lucy, M. C., Hauser, S. D., Eppard, P. J., Krivi, G. G. and Collier, R. J. 1991. Genetic polymorphism within the bovine somatotropin(bST) gene detected by polymerase chain reaction and endonuclease digestion. J. Dairy Sci. 74(Suppl. 1): 284(Abstr.).
  16. Lucy, M. C., Hauser, S. D., Eppard, P. J., Krivi, G. G., Clark, J. H., Bauman, D. E. and Collier, R. J. 1993. Variants of somatotropin in cattle : gene frequencies in major dairy breeds and associated milk production. Domest. Anim. Endocrinol. 10:325.
  17. Rhynes, W. E. and Ewing, L. L. 1973. Testicular endocrine function in hereford bulls exposed to high ambient temperature. Endocrinology. 82:509.
  18. Sabour, M. P., Lin, C. Y. and Smith, C. 1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. J. Anim. Breed. Genet. 114:435.
  19. Schlee, P., Graml, R., Schallenberger, E., Schams, D., Rottman, O., Olbrich-Bludau, A. and Pirchner, F. 1994. Growth hormone and insulin-like growth factor I concentration in bulls of various growth hormone genotypes. Theor. Appl. Genet. 88:497.
  20. Spiteri-Grech, J. and Nieschlag, E. 1992. The role of growth hormone and insulin-like growth factor I in the regulation of male reproductive function. Horm-Res. 38(Suppl 1):22.
  21. 정의룡, 임태진, 한상기. 1996. 젖소에서 성장호르몬과 Prolactin 유전자의 PCR-RFLP 표지와 생산형질간의 연관성 분석. 한국축산학회지. 38: 321.

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