Bilolgical Activities of Conjugated Linoleic Acid (CLA) and Animal Products

Conjugated Linoleic Acid (CLA)의 생리활성과 축산식품

  • Hur, S.J. (Division of Animal Science, College of Agriculture) ;
  • Lee, J.I. (Gyeongsang National University, Advanced Swine Research Institute) ;
  • Ha, Y.L. (Division of Agricultural Chemistry, College of Agriculture) ;
  • Park, G.B. (Division of Animal Science, College of Agriculture) ;
  • Joo, S.T. (Division of Animal Science, College of Agriculture)
  • Published : 2002.08.31


Conjugated linoleic acid(CLA) is a collective term for a group of positional (c8, c10; c9, c11; c10, c12, and c11, c13) and geometric(cis,cis; cis,trans; trans,cis; and trans,trans) isomers of octadecadienoic acid (linoleic acid) with conjugated double bond system. CLA has been shown to have a variety of biological effects. Major effects of CLA on health, such as anti-cancer, anti-oxidation, anti-atherosclerosis and improving immuno-responses, might be derived or partially derived from the alternated lipid metabolism after CLA feeding. Most of studies on the effect of CLA on fat metabolism are concentrated on rats, mice, pigs and other mammals. The CLA inhibited carcinogen-induced neoplasia in several animal models and inhibited the proliferation of human malignant melanoma, colorectal and breast cancer cells and CLA reduced the atherosclerosis. Several studies have determined the antioxidant property of CLA; however, the property still remains controversial. Some of the studies have shown that CLA acted as an antioxidant, whereas some other studies have demonstrated that CLA might be a prooxidant. Several studies suggested that CLA could reduce fat accumulation in mammals. CLA was suggested to promote muscle growth and reduce fat deposition in mouse, and improve feed efficiency in rats. CLA has been shown to inhibit the activity of stearoyl-CoA reductase. CLA also reduced the content of arachidonic acid. Since arachidonic acid, and eicosapentaenoic acid (EPA) and docosahexenoic acid (DHA) are synthesized by different pathways, reducing the synthesis of arachidonic acid may not mean reducing that of EPA and DHA. Many sutdies have been shown biological effects of CLA. Therefore, further research is needed to answer the following questions: 1) how to synthesize the new CLA by new methods, 2) why CLA has shown biological effects, 3) how to increase CLA effects in animal products.

이상의 연구 결과들을 요약해보면 CLA는 생체내외에서 다양한 생리활성 효과를 가지는 것을 알 수 있다. CLA의 주요 효과는 유방암, 췌장암, 피부암 및 대장암을 억제하는 효과, 동물체내에서의 면역력증강 효과, 동맥경화증에 대한 효과, 지방축적 억제효과, 체내 지방분해 촉진효과 및 식육에서의 항산화 효과와 육색안정 효과 등으로 요약된다. 그러므로 CLA는 건강보조식품으로 혹은 축산식품을 비롯한 다양한 식품의 기능성 첨가제로써의 충분한 가능성을 가지고 있는 것으로 사료된다. 그러나 이러한 CLA의 효과는 실험에 따라 다소간의 차이를 나타내고 또한 CLA의 생리활성 기작에 대한 정확한 이해가 부족한 실정이다. 따라서 향후 CLA와 관련하여 다음과 같은 연구가 필요할 것으로 사료된다. 1) 새로운 CLA 이성체의 발견 및 CLA 합성방법에 대한 연구 2) CLA가 가지는 각각의 생리활성 효과에 대한 정확한 기작을 밝히는 연구 3) CLA 이성체간의 생리활성 효과 차이에 대한 연구 4) CLA의 생리활성 효과를 극대화 할 수 있는 이성체간의 비율에 대한 연구 5) 기능성 식품첨가제로써의 CLA를 효과적으로 이용하는 방법 연구 6) CLA를 식용가축 및 축산물에 효과적으로 축적시키는 방법 연구 7) CLA를 이용한 육제품 및 유제품 개발 연구 8) 사람에 있어 CLA 효과에 대한 임상 연구.



  1. Ahn, D. U., Sell, J. L., Jo, C., Chamruspollert, M. and Jeffrey, M. 1999. Effect of dietary conjugated linoleic acid on the quality charac- teristics of chicken eggs during refrigerated storage. Poultry Sci. 78:922-928.
  2. Aii, T., Matsuzaki, S., Sakamoto, K., Hayasawa, H., Shimizu, T. and Ishida, S. 1999. A newly discovered effect of conjugated linoleic acid: Damage to the hatchability of fowl eggs. J. Ani. Sci. 70:246-247.
  3. Azain, M. J., Hausman, D. B., Sisk, M. B., Flatt, W. P. and Jewell, D. E. 2000. Dietary conjugated linoleic acid reduces rat adipose tissue cell size rather than cell number. J. Nutrition. 130:1548-1554.
  4. Banni, S., Angioni, E., Contini, M. S., Carta, G., Casu, V., Iengo, G. A., Melis, M. P., Deiana, M., Dessi, M. A. and Corongiu, F. P. 1998. Conjugated linoleic acid and oxidative stress. J. Am. Oil Chem. Soc. 75: 261-267.
  5. Bauman, D. E., Barbano, D. M., Dwyer, D. A. and Griinari, J. M. 2000. Technical note: production of butter with enhanced conjugated linoleic acid for use in biomedical studies with animal models. J. Dairy Sci. 83:2422-2425.
  6. Baumgard, L. H., Corl, B. A., Dwyer, D. A., Saebo, A. and Bauman, D. E. 2000. Identifi- cation of he conjugated linoleic acid isomer that inhibits milk fat synthesis. Ame. J. Phys. 278: R179-R184.
  7. Bee, G. 2000a. Dietary conjugated linoleic acid consumption during pregnancy and lactation influences growth and tissue composition in weaned pigs. J. Nutrition. 130:2981-2989.
  8. Bee, G. 2000b. Dietary conjugated linoleic acid alter adipose tissue and milk lipids of pregnant and lactating sows. J. Nutrition. 130:2292-2298.
  9. Belury, M. A., Nickel, K. P., Bird, C. E. and Wu, Y. 1996. Dietary conjugated linoleic acid modulation of phorbol ester skin tumor promotion. Nutrition Canc. 26:149-157.
  10. Benzamin, H., Strokson, J. M., Albright, K. and Pariza, M. W. 1990. TPA-mediated induction of ornithine decarboxylase activity in mouse forestomach and its inhibition by conjugated dienoic derivatives of linoleic acid. FASEB J. 4: 1403.
  11. Berdeaux, O., Christie, W. W., Gunstone, F. D. and Sebedio. J. L. 1997. Large-scale synthesis of methyl cis-9, trans-11-octadecadienoate from methyl ricinoleate. J. Am. Oil Chem. Soc. 74: 1011-1015.
  12. Bretillon, L., Chardigny, J. M., Gregoire, S., Berdeaux, O. and Sebedio, J. L. 1999. Effects of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro. Lipids. 34:965-969.
  13. Burge, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52:302- 303.
  14. Chamruspollert, M. and Sell, J. L. 1999. Transfer of dietary conjugated linoleic acid to egg yolks of chickens. Poultry Sci. 78:1138-1150.
  15. Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L. and Pariza, M. W. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Comp. Anal. 5:185-197.
  16. Chin, S. F., Storkson, J. M., Albright, K. J., Cook, M. E. and Pariza, M. W. 1994. Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. J. Nutrition. 124: 2344-2349.
  17. Choi, Y., Kim, Y. C., Han, Y. B., Park, Y., Pariza, M. W. and Ntambi, J. M. 2000. The trans-10, cis-12 isomer of conjugated linoleic acid down regulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes. J. Nutrition. 130:1920-1924.
  18. Chouinard, P. Y., Corneau, L., Butler, W. R., Chilliard, Y., Drackley, J. K. and Bauman, D. E. 2001. Effect of dietary lipid source on conjugated linoleic acid concentrations in milk fat. J. Dairy Sci. 84:680-690.
  19. Cook, M. E., Miller, C. C., Park, Y. and Pariza, M. 1993. Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poultry Sci. 72:1301-1305.
  20. de-Deckere, E. A. M., van Amelsvoort, J. M. M., McNeill, G. P. and Jones, P. 1999. Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. Brit. J. Nutrition. 82:309-317.
  21. DeLany, J. P., Blohm, F., Truett, A. A., Scimeca, J. A. and West, D. B. 1999. Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Ame. J. Phys. 276:R1172-R1179.
  22. DeLany, J. P. and West, D. B. 2000. Changes in body composition with conjugated linoleic acid. J. Ame. Coll. Nutrition. 19:487S-493S.
  23. Dhiman, T. R., Anand, G. R., Satter, L. D. and Pariza, M. W. 1999. Conjugated linoleic acid content of milk from cows fed different diets. J. Dairy Sci. 82:2146-2156.
  24. Dormandy, T. L. and Wickens, D. G. 1987. The experimental and clinical pathology of diene conjugation, Chem. Phys. Lipids. 45: 353-364.
  25. Du, M., Ahn, D. U., Nam, K. C. and Sell, J. L. 2000a. Influence of dietary conjugated linoleic acid on volatile profiles, color and lipid oxidation of irradiated raw chicken meat. Meat Sci. 56: 387-395.
  26. Du, M., Ahn, D. U., Nam, K. C. and Sell, J. L. 2001a. Volatile profiles and lipid oxidation of irradiated cooked chicken meat from laying hens fed with diets containing conjugated linoleic acid. Poultry Sci. 80: 235-241.
  27. Du, M., Ahn, D. U. and Sell, J. L. 1999. Effect of dietary conjugated linoleic acid on the composition of egg yolk lipids. Poultry Sci. 78: 1639-1645.
  28. Du, M., Ahn, D. U. and Sell, J. L. 2000b Effect of dietary conjugated linoleic acid (CLA) and linoleic/linolenic acid ratio on polyunsaturated fatty acid status in laying hens. Poultry Sci. 79: 1749-1756.
  29. Du, M. and Ahn, D. U. 2001b. Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poultry Sci. 81:428-433.
  30. Du, M., Nam, K. C. Hur, S. J., Ismail, H. and Ahn, D. U. 2002. Effect of dietary conjugated linoleic acid, irradiation, and packaging conditions on the quality characteristics of raw broiler breast fillets. Meat Sci. 60:9-15.
  31. Dugan, M. E. R., Aalhus, J. L., Jeremiah, L. E., Kramer, J. K. G. and Schaefer, A. L. 1999. The effects of feeding conjugated linoleic acid on subsequent pork quality. Can. J. Ani. Sci. 79: 45-51.
  32. Dugan, M. E. R., Aalhus, J. L., Schaefer, A. L. and Kramer, J. K. G. 1997. The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can. J. Ani. Sci. 77: 723-725.
  33. Esterbauer, H., Gebicki, J., Puhl, H. and Juergens, G. 1992. The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Radical Biol. and Med. 13: 341-390.
  34. Evans, M., Geigerman, C., Cook, J., Curtis, L., Kuebler, B. and McIntosh, M. 2000. Conjugated linoleic acid suppresses triglyceride accumulation and induces apoptosis in 3T3-L1 preadipocytes. Lipids. 35:899-910.
  35. Gray, J. I. and Pearson, A. M. 1994. Lipid- derived off-flavors in meat-formation and inhibition. In: Shahidi, F. (ed.) Flavor of Meat and Meat Products. Chapman & Hall, Glasgow, pp. 116-143.
  36. Greene, B. E. 1969. Lipid oxidation and pigment changes in raw beef. J. Food Sci. 34: 110-113.
  37. Ha, Y. L., Grimm, N. K. and Pariza, M. W. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis. 8:1881-1887.
  38. Ha, Y. L. and Pariza, M. W. 1991. Naturally- occurring novel anticarcinogens: conjugated dienoic derivatives of linoleic acid (CLA). J. Kor. Soc. Food Nutrition. 24:401-407.
  39. Ha, Y. L., Storkson, J. M. and Pariza, M. W. 1990. Inhibitation of benzo(a)pyrene-induced mouse forestomach neoplasis by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50:1097-1101.
  40. Halliwell, B. and Gutteridge, J. M. C. 1989. Lipid peroxidation: a radical chain reaction. In Free Radical in Biology and Medicine, 2nd. Clarendon Press, London, 1989, pp189.
  41. Ip C., Chin, S. F., Scimeca, J. A. and Pariza, M. W. 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res. 51: 6118-6124.
  42. Ip C., Scimeca, J. A. and Thompson, H. J. 1994. Conjugated linoleic acid. A powerful anticarcinogen from animal fat sources. Cancer. 74:1050-1054.<1050::AID-CNCR2820741512>3.0.CO;2-I
  43. Ip, C., Scimeca, J. A. and Thompson, H. J. 1995. Effect of timing and duration of dietary conjugated linoleic acid on mammary cancer prevention. Nutr. Canc. 24:241-247.
  44. Ito, N. and Hirose, M. 1989. Anti-oxidants: carcinogenic and chemopreventive properties. In : Vande Woude GF, Klein G, eds. Adv. Cancer Res. 53:247-302. San Diego, CA: Academic Press.
  45. Jiang, J., Bjorck, L. and Fonden, R. 1998. Production of conjugated linoleic acid by dairy starter cultures. J. Appl. Micro. 85:95-102.
  46. Jeroen, J. M., Berg, V. D., Cook, N. E. and Tribble, D. L. 1995. Reinvestigation of the antioxidant properties of conjugated linoleic acid. Lipids. 30:599-605.
  47. Jo, C. and Ahn, D. U. 1998. Fluorometric analysis of 2-thiobarbituric acid reactive sub- stances in turkey. Poultry Sci. 77:475-480.
  48. Johnson, F. C. 1982. Carcinogenesis, vascular disease and the free radical reaction. Nutr. Cancer. 3:117-121.
  49. Jones, P. A., Lea, L. J. and Pendlington, R. U. 1999. Investigation of the potential of conjugated linoleic acid (CLA) to cause peroxisome proliferation in rats. Food Chem. Toxi. 37:1119- 1125.
  50. Jones, S., Ma, D. W. L., Robinson, F. E., Field, C. J. and Clandinin, M. 2000. Isomers of conjugated linoleic acid (CLA) are incorporated into egg yolk lipids by CLA-fed laying hens. J. Nutrition. 130:2002-2005.
  51. Joo, S. T., Lee, J. I., Ha, Y. L. and Park, G. B. 2002. Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color and water-holding capacity of pork loin. J. Ani. Sci. 80:108-112.
  52. Kavanaugh, C. J., Liu, K. L. and Belury, M. A. 1999. Effect of dietary conjugated linoleic acid phorbol ester-induced PGE2 production and hyperplasia in mouse epidermis. Nutr. Cancer. 33: 132-138.
  53. Kelper, C. R., Hirons, K. P., McNeill, J. J. and Tove, S. B. 1966. Intermediates and products of the biohydrogenation of linoleic acid by butyri- vibrio fibrisolvens. J. Biol. Chem. 241:1350- 1354.
  54. Kritchevsky, D., Tepper, S. A., Wright, S., Tso, P. and Czarnecki, S. K. 2000. Influence of conjugated linoleic acid (CLA) on establishment and progression of atherosclerosis in rabbits. J. Am. Colle. Nutrition. 19:472S-477S.
  55. Latour, M. A., Devitt, A. A., Meunier, R. A., Stewart, J. J. and Watkins, B. A. 2000b. Effects of conjugated linoleic acid. 2. Embryonic and neonatal growth and circulating lipids. Poultry Sci. 79:822-826.
  56. Lee, K. N., Kritchevsky, D. and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 108:19-25.
  57. Lee, K. N., Pariza, M. W. and Ntambi, J. M. 1998. Conjugated linoleic acid decreases hepatic stearoyl-CoA desaturase mRNA expression. Bioche. Biophys. Res. Comm. 248:817-821.
  58. Leng, G. C., Horrobin, D. F., Fowkes, F. G. R., Smith, F. B., Lowe, G. D. O., Donnan, P. T. and Ells, K. 1994. Plasma essential fatty acids, cigarette smoking, and dietary antioxidants in peripheral arterial disease. A population-based case-control study. Atherosclerosis Thromb. 14: 471-478.
  59. Lin, T. Y., Lin, C. W. and Lee. C. H. 1999. Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid. Food Chem. 67:1-5.
  60. Li Y. and Watkins, B. A. 1998. Conjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acids. Lipids. 33: 417-425.
  61. Liu, K. L. and Belury, M. A. 1998. Conjugated linoleic acid reduces arachidonic acid content and PGE2 synthesis in murine keratinocytes. Cancer Lett. 127: 15-22.
  62. Loor, J. J. and Herbein, J. H. 1998. Exogenous conjugated linoleic acid isomers reduce bovine milk fat concentration and yield by inhibiting de novo fatty acid synthesis. J. Nutrition. 128: 2411-2419.
  63. Ma, D. W. L., Wierzbicki, A. A., Field, C. J. and Clandinin, M. T. 1999. Preparation of conjugated linoleic acid from safflower oil. J. Am. Oil Chem. Soc. 76:729-730.
  64. Martin, J. C., Gregoire, S., Siess, M. H., Genty, M., Chardigny, J. M., Berdeaux, O., Juaneda, P. and Sebedio, J. L. 2000a. Effects of conjugated linoleic acid isomers on lipid-metabolizing enzymes in male rats. Lipids. 35:91-98.
  65. Martin, J. C., Sebedio, J. L., Caselli, C., Pimont, C., Martine, L. and Bernard, A. 2000b. Lymphatic delivery and in vitro pancreatic lipase hydrolysis of glycerol esters of conjugated linoleic acids in rats. J. Nutrition. 130:1108- 1114.
  66. Miner, J. L., Cederberg, C. A., Nielsen, M. K., Chen, X. and Baile, C. A. 2001. Conjugated linoleic acid (CLA), body fat, and apoptosis. Obe. Res. 9:129-134.
  67. Mooney, M. A., Vaughn, D. M., Reinhart, G. A., Power, R. D., Wright, J. C., Hoffman, C. E., Swaim, S. F. and Baker, H. J. 1998. Evaluation of the effects of omega-3 fatty acid-containing diets on the inflammatory stage of wound healing in dogs. Am. J. Vet. Res. 59:859-863.
  68. Moya-Camarena, S. Y. and Belury, M. A. 1999. Species differences in the metabolism and regu- lation of gene expression by conjugated linoleic acid. Nutr. Rev. 57:336-340.
  69. Mueller, H. L., Kirchgessner, M., Roth, F. X. and Stangl, G. I. 2000. Effect of conjugated linoleic acid on energy metabolism in growing- finishing pigs. J. Ani. Phys. Ani. Nutrition. 83: 85-94.
  70. Mueller, H. L., Stangl, G. I. and Kirchgessner, M. 1999. Energy balance of conjugated linoleic acid-treated pigs. J. Ani. Phys. Ani. Nutrition. 81:150-156.
  71. Nichols, P. L., Riemenschneider, R. W. Jr. and Herb, S. F. 1950. Kinetics of alkali isomerization of linoleic, linolenic and arachidonic acids, J. Am. Oil Chem. Soc. 27:329-336.
  72. Nicolosi, R. J., Courtemanche, K. V., Laitinen, L., Scimeca, J. A. and Huth, P. J. 1993. Effect of feeding diets enriched in conjugated linoleic acid on lipoproteins and aortic atherogenesis in hamsters. Circulation 88 Suppl. 2458.
  73. Nicolosi, R. J., Rogers, E. J., Kritchevsky, D., Scimeca, J. A. and Huth, P. J. 1997. Dietary conjugated linoleic acid reduces plasma lipo- proteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 22:266- 277.
  74. O’-Quinn, P. R., Nelssen, J. L., Goodband, R. D., Unruh, J. A., Woodworth, J. C., Smith, J. S. and Tokach, M. D. 2000. Effects of modified tall oil versus a commercial source of conjugated linoleic acid and increasing levels of modified tall oil on growth performance and carcass characteristics of growing-finishing pigs. J. Ani. Sci. 78:2359-2368.
  75. Padley, F. B., Gunstone, F. D. and Harwood, J. L. 1994. Occurrence and characteristics of oils and fats. In; The Lipid Handbook, (Gunstone, F. D., Harwood, J. L., Padley F. B. eds.) 2nd ed., pp. 47-224, Chapman and Hall, London.
  76. Park, Y., Albright, K. J., Storkson, J. M., Cook, M. E. and Pariza, M. W. 1999a. Changes in body composition during feeding and withdrawal of dietary conjugated linoleic acid. Lipids. 34: 243-248.
  77. Park, Y., Albright, K. J., Storkson, J. M., Liu, W. and Pariza, M. W. 1999b. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids. 34:235-241.
  78. Park, Y., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E. and Pariza, M. W. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids. 32:853-858.
  79. Park, Y., Storkson, J. M., Ntambi, J. M., Cook, M. E., Sih, C. J. and Pariza, M. W. 2000. Inhibition of hepatic stearoyl-CoA desaturase activity by trans-10, cis-12 conjugated linoleic acid and its derivatives. Biochi. Biophy. Acta. 1486:285-292.
  80. Parodi, P. W. 1997. Cow's milk fat componants as potential anticarcinogenic agents. J. Nutrition. 127:1055-1060.
  81. Petrik, M. B. H., McEntee, M. F., Johnson, B. T., Obukowicz, M. G. and Whelan, J. 2000. Highly unsaturated (n-3) fatty acids, but not alpha-linolenic, conjugated linoleic or gamma- linolenic acids, reduce tumorigenesis in ApcMin/+ mice. J. Nutrition. 130:2434-2443.
  82. Renerre, M. and Labadie, J. 1993. Fresh meat packaging and meat quality. Proc. 39th ICoMST, p 361. Calgary.
  83. Sakono, M., Miyanaga, F., Kawahara, S., Yamauchi, K., Fukuda, N., Watanabe, K., Iwata, T. and Sugano, M. 1999. Dietary conjugated linoleic acid reciprocally modifies ketogenesis and lipid secretion by the rat liver. Lipids. 34:997- 1000. Hur et al. ; Biological Activities of Conjugated Linoleic Acid(CLA) and Animal Products
  84. Schaefer, D. M., Liu, Q., Faustman, C. and Yin, M. C. 1995. Supranutritional administration of vitamins E and C improves antioxidant status of beef. J. Nutrition. 125:1792S-1798S.
  85. Scimeca, J. A. 1998. Toxicological evaluation of dietary conjugated linoleic acid in male Fisher 344 rats. Food Chem. Toxi. 36:391-395.
  86. Scott, M. G. 1987. Monounsaturated fatty acids, plasma cholesterol, and coronary heart disease. Am. J. Clin. Nutrition. 45:1168-1175.
  87. Sehat, N., Rickert, R., Mossoba, M. M., Kramer, J. K., Yurawecz, M. P., Roach, J. A. G., Adlof, R. O., Morehouse, K. M., Fritsche, J., Eulitz, K. D., Steinhart, H. and Ku, Y. 1999. Improved separation of conjugated fatty acid methyl esters by silver-ion high-performance liquid chromato- graphy. Lipids. 34: 407-413.
  88. Sell, J. L., Jin, S. and Jeffrey, M. 2001. Meta- bolizable energy value of conjugated linoleic acid for broiler chicks and laying hens. Poultry Sci. 80:209-14.
  89. Shultz, T. D., Chew, B. P. and Seaman, W. R. 1992. Differential stimulatory and inhibitory responses of human mcf-7 breast cancer cells to linoleic acid and conjugated linoleic acid in culture. Anticancer. Res. 12: 2134-2145.
  90. Stangl, G. I. 2000. High dietary levels of a conjugated linoleic acid mixture alter hepatic glycerophospholipid class profile and cholesterol- carrying serum lipoproteins of rats. J. Nutrition. Biochem. 11:184-191.
  91. Stevens, L. 1996. Avian Biochemistry and Molecular Biology, Cambridge University Press, London, pp50.
  92. Takenoyama, S., Kawahara, S., Murata, H. and Yamauchi, K. 1999. Investigation of some preparation procedures of fatty acid methyl esters for capillary gas-liquid chromatographic analysis of conjugated linoleic acid in meat. J. Ani. Sci. 70:336-342.
  93. Twibell, R. G., Watkins, B. A., Rogers, L. and Brown, P. B. 2000. Effects of dietary conjugated linoleic acids on hepatic and muscle lipids in hybrid striped bass. Lipids. 35:155-161.
  94. West, D. B., Blohm, F. Y., Truett, A. A. and DeLany, J. P. 2000. Conjugated linoleic acid persistently increases total energy expenditure in AKR/J mice without increasing uncoupling protein gene expression. J. Nutrition. 130:2471- 2477.
  95. West, D. B., Delany, J. P., Camet, P. M., Blohm, F., Truett, A. A. and Scimeca, J. 1998. Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am. J. Phys. 275: R667-R672.
  96. Yagi. K. 1987. Lipid peroxides and human diseases. Chem. Phys. Lipids. 45:337-351.
  97. Yin, M. C. and Faustman, C. 1993. The influence of temperature, pH, and phospholipid composition upon the stability of myoglobin and phospholipid: a liposome model. J. Agri. Food Chem. 41:853-857.
  98. Yurzwecz, M. P., Hood, J. K., Mossoba, M. M., Roach, J. A. and Ku, Y. 1995. Furan fatty acid determined as oxidation products of conjugated octadecadienoic acid. Lipids. 30:595-598.
  99. 박구부, 문성실, 이정일, 하영래, 주선태. 2001. 식물성유와 동물성유 CLA가 유화형 Sausage의 지방산패도, 육색 및 지방산 조성의 변화에 미치는 영향. 한국축산식품학회지. 21:71-79.
  100. 박구부, 예병화, 문성실, 진상근, 이정일, 주선태. 2000. Conjugated linoleic acid가 Myoglobin의 산화 안전성에 미치는 효과. 동물자원과학회지. 42:905-914.
  101. 박구부, 이정일, 하경희, 예병화, 허선진, 신택순, 주선태. 1999. Conjugated linoleic acid가 냉장돈육 패티의 아질산염, 콜레스테롤 및 지방산 함량 감소에 미치는 영향. 한국축산식품학회지. 19:169-178.
  102. 이정일, 주선태, 박태선, 신택순, 하영래, 박구부. 1999a. Conjugated linoleic acid (CLA)가 축적된 계육의 저장기간중 이화학적 특성 변화. 한국축산식품학회지. 19:88-99.
  103. 이정일, 주선태, 최병대, 하영래, 하정기, 박구부. 1999b. Conjugated linoleic acid (CLA) 급여기간이 계육의 CLA 함량과 지방산 조성에 미치는 영향. 한국축산학회지. 41:375-386.
  104. 이정일, 최진성, 박준철, 문흥길, 김영화, 박종대, 주선태, 박구부. 2001a. Conjugated linoleic acid (CLA) 급여가 돈육의 조직적, 관능적 특성에 미치는 영향. 한국축산식품학회지. 21:349- 357.
  105. 이정일, 최진성, 박종대, 박준철, 김영화, 문홍길, 주선태, 박구부, 2001b. Conjugated linoleic acid (CLA) 급여가 돈육 품질에 미치는 효과. 동물자원과학회지. 43:735-746.
  106. 주선태, 이정일, 하경희, 하영래, 박구부. 2000. Conjugated linoleic acid 첨가가 돈육 패티의 품질특성에 미치는 효과. 한국식품과학회. 32:62- 68.

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