Clinical and Experimental Reproductive Medicine

  • 제29권3호
  • /
  • Pages.195-200
  • /
  • 2002
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

인간난포에서 Apoptosis 관련 단백질 및 Apoptotic 세포의 면역학적 발현

The Immunologic Expression of Apoptosis Related Proteins and Apoptotic Cells in Human Ovarian Follicles

  • 박성록 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실) ;
  • 이병석 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실) ;
  • 양우익 (연세대학교 의과대학 병리학교실) ;
  • 김종화 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실) ;
  • 박병주 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실) ;
  • 박기현 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실) ;
  • 조동제 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실) ;
  • 송찬호 (연세대학교 의과대학 부속 영동 세브란스 병원 산부인과학교실)
  • Park, Sung-Rok (Department of Obstetrics and Gynecology, Yonsei University College of Medicine) ;
  • Lee, Byung-Seok (Department of Obstetrics and Gynecology, Yonsei University College of Medicine) ;
  • Yang, Woo-Ick (Department of Pathology, Yonsei University College of Medicine) ;
  • Kim, Jong-Hwa (Department of Obstetrics and Gynecology, Yonsei University College of Medicine) ;
  • Park, Byung-Joo (Department of Obstetrics and Gynecology, Yonsei University College of Medicine) ;
  • Park, Ki-Hyun (Department of Obstetrics and Gynecology, Yonsei University College of Medicine) ;
  • Cho, Dong-Jae (Department of Obstetrics and Gynecology, Yonsei University College of Medicine) ;
  • Song, Chan-Ho (Department of Obstetrics and Gynecology, Yonsei University College of Medicine)
  • 발행 : 2002.09.30
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초록

Objective : To investigate the expression of apoptosis related proteins and apoptotic cells on the human ovarian follicles. Materials and Methods: Thirty five Formalin-fixed paraffin-embedded human ovarian tissue blocks were selected from the surgical pathology files of the department of pathology, College of Medicine, Yonsei University, for the period from 1996 to 1998. All specimen were from premenopausal women aged from $32{\sim}45$. Ovarian tissues were collected from the patients performing hysterectomy for benign uterine diseases. Immunohistochemical staining was performed for the detection of DNA fragmented cell, Bcl-2, Bax, Fas and Fas-ligand. Results: Bcl-2 and bax were not expressed on the surrounding cells and oocyte of the primary, primordial and preantral follicles. Fas and Fas-ligand (Fas-L) were not expressed on the surrounding cells on the primordial and primary follicles. But expressed on the surrounding granulosa cells and oocyte in the primordial and primary follicles. In the healthy follicles, Bcl-2 was expressed on the granulosa cells, however, Bax was not expressed. DNA fragmented cells were expressed on the inner granulosa cell layer of atretic follicles. Conclusion: Fas, Fas-ligand, and Bax may be responsible for the follicular atresia and Bcl-2 may be involved in the follicular survival in the human ovary.

키워드

참고문헌

  1. Kaipia A, Hsueh AJ. Regulation of ovarian follicle atresia. Annu Rev Physiol 1997; 59: 349-63 https://doi.org/10.1146/annurev.physiol.59.1.349
  2. Palumbo A, Yeh J. In situ localization of apoptosis in the rat ovary. Biol Reprod 1994; 51: 885-95
  3. Tilly JL, Kowalski KI, Johnson AL, Hsueh AJW. Involvement of apoptosis in ovarian follicular atresia and postovulatory regression. Endocrinology 1991; 129: 2799-801 https://doi.org/10.1210/endo-129-5-2799
  4. Funayama Y, Sasano H, Suzuki T, Tamura M, Fukaya T, Yajima A. Cell turnover in normal cycling human ovary. J Clin Endocrionol Metab 1996; 81: 828-34 https://doi.org/10.1210/jc.81.2.828
  5. Yuan W, Giudice LC. Programmed cell death in human ovary is a function of follicle and corpus luteum status. J Clin Endocrinol Metab 1997; 82: 3148-55 https://doi.org/10.1210/jc.82.9.3148
  6. Owens GP, Hahn WE, Cohen JJ. Identification of mRNAs associated with programmed cell death in immature thymocytes. Mol Cell Biol 1991; 11: 4177-88 https://doi.org/10.1128/MCB.11.8.4177
  7. Lee BS, Yeh J. The molecular basis of follicular atresia: Apoptosis as a possible mechanism. Adv Contracep Deliv Sys 1996; 2: 269-82
  8. Tilly JL, Tilly KI, Kenton ML, Johnson AL. Expression of members of the bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin mediated inhibition of granulosa cell apoptosis is associated with decreased bax and constitutive bcl-2 and bcl-Xlong messenger ribonucleic acid levels. Endocrinology 1995; 136: 232-41 https://doi.org/10.1210/en.136.1.232
  9. Tilly KI, Banerjee S, Banerjee PP, Tilly JL. Expression of the p53 and Wilms' tumor suppressor genes in the rat ovary: gonadotropin repression in vivo and immunohistochemical localization of nuclear p53 protein to apoptotic granulosa cells of atretic follicles. Endocrinology 1995; 136: 1394-402 https://doi.org/10.1210/en.136.4.1394
  10. Ratts VS, Flaws JA, Kolp R, Sorenson CM, Tilly JL. Ablation of bcl-2 gene expression decreases the number of oocytes and primordial follicles established in the post-natal female mouse gonad. Endocrinology 1995; 136: 3665-8 https://doi.org/10.1210/en.136.8.3665
  11. Fukaya T, Funayama Y, Muakami T, Sugawara J, Yajima A. Does apoptosis contribute follicular at - resia and luteal regression in human ovary? Horm Res 1997; 48 (suppl 3): 20-6
  12. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2/Heterodimerizes In Vivo with a Conserved Homolog, Bax, That Accelerates Programmed Cell Death. Cell 1993; 74: 609-19 https://doi.org/10.1016/0092-8674(93)90509-O
  13. Itoh N, Yonehara S, Ishii A, Yonehara M, Mizushima S, Sameshima M, et al. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell 1991; 66: 233-43 https://doi.org/10.1016/0092-8674(91)90614-5
  14. Hakuno N, Koji T, Yano T, Kobayashi N, Tsutsumi O, Taketani Y, et al. Fas/APO-1/CD95 system as a mediator of granulosa cell apoptosis in ovarian follicle atresia. Endocrinology 1996; 137: 1938-48 https://doi.org/10.1210/en.137.5.1938
  15. Quirk SM, Cowan RG, Joshi SG, Henrikson KP. Fas antigen-mediated apoptosis in human granulosa/luteal cells. Biol Reprod 1995; 52: 279-87 https://doi.org/10.1095/biolreprod52.2.279
  16. Sakamaki K, Yoshida H, Nishimura Y, Nishikawa S, Manabe N, Yonehara S. Involvement of Fas antigen in ovarian follicular atresia and luteolysis. Mol Reprod Dev 1997; 47: 11-8 https://doi.org/10.1002/(SICI)1098-2795(199705)47:1<11::AID-MRD2>3.0.CO;2-T
  17. Kondo H, Mauro T, Peng X, Mochizuki M. Immunological evidence for the expression of the Fas antigen in the infant and adult human ovary during follicular regression and atresia. J Clin Endocrinol Metab 1996; 81: 2702-10 https://doi.org/10.1210/jc.81.7.2702