The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Effects & Mechanism of Omija-tang on Oxidative Stress-Induced Death of H9c2 Cardiomyoblast Cell

심근세포의 산화적 손상에 대한 오미자탕의 효과 및 작용기전 연구

  • 황보연 (원광대학교 한의과대학 내과학교실) ;
  • 양경석 (원광대학교 한의과대학 내과학교실) ;
  • 이상관 (원광대학교 한의과대학 내과학교실) ;
  • 이기상 (원광대학교 한의과대학 내과학교실) ;
  • 문병순 (원광대학교 한의과대학 내과학교실) ;
  • 신선호 (원광대학교 한의과대학 내과학교실)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The water extract of Omija-tang (OMIT) has traditionally been used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of OMJT rescues cells from these damages. Therefore, this study was designed to investigate the protective mechanisms of OMJT on oxidative stress-induced toxicity in H9c2 cardiomyoblast cells. Methods: Treatments of $H_2O_2$, or $ZnC_{12}$ markedly induced death of H9c2 cardiomyoblast cells in a dose-dependent manner. The characteristics of oxidative stress-induced death of H9c2 showed apparent apoptotic features such as DNA fragmentation. OMJT significantly reduced both ${H_2O_2}-induced$ cell death and chromatin fragmentation. The decrease of B치-XL expression by $H_2O_2$ were inhibited by OMJT. In addition, the increase of Bcl-XS expression was also inhibited by OMJT. In particular, Fas expression, which is generally recognized as cell death-inducing signal by Fas/FasL interaction, was markedly increased by H2O2 in a time-dependent manner. Also, the expression profile of proteins in Chang cells were screened by using two-dimensional (2-D) gel electrophoresis. Among 300 spots resolved in 2-D gels; the comparison of control versus apoptotis cells revealed that signal intensity of 6 spots decreased and 11 spots increased. Results and Conclusions: Taken together, this study suggests that the protective effects of the water extract of OMJT against oxidative damages may be mediated by the modulation of Bcl-XL/S Fas expression.

Keywords

References

  1. 경악전서(하) 장개빈
  2. 현대중의생리학기초 이종박
  3. 중의방제대사전(제삼책) 팽부인
  4. 본초학 신민교
  5. 중의내과학 장백수
  6. 임상배합본초학 강병수
  7. Proc Natl Acad Sci USA. v.90 Oxidants, antioxidants and the degenerative diseases of aging Ames BN;Shigenaga MK;Hagen TM
  8. Toxicol Appl Pharmacol v.159 no.3 Metallothionein and apoptosis during differentiation of myoblasts to myotubes: protection against free radical toxicity Apostolova MD;Ivanova IA;Cherian MG
  9. Biochem Cell Biol. v.78 no.1 Signal transduction pathways and nuclear translocation of zinc and metallothionein during differentiation of myoblasts Apostolova MD;Ivanova IA;Cherian MG
  10. Mol Cell Biochem. Apoptosis in the heart:when and why? Bromme HJ;Holtz J
  11. Arch Biochem Biophys v.373 no.1 Hydrogen peroxide dose dependent induction of cell death or hypertrophy in cardiomyocytes Chen QM;Tu VC;Wu Y;Bahl JJ
  12. In free radicals in biology and medicine(2nd Ed) Oxygen is poisonous-an introduction oxygen toxocity and free radicals Halliwell B;Gutteridge JMC
  13. 대한한방내과학회지 v.23 no.2 오미자탕이 심근세포에 미치는 영향 최진영
  14. 원광대학교 박사학위논문 생맥산이 백서의 심혈관계에 미치는 영향 김세길
  15. 경희대학교 석사학위논문 생맥산이 스포츠 음료로서 운동수행 능력과 혈액학적 변화에 미치는 영향 이응세
  16. 대한한의학회지 v.192 산소분압에 의한 심근세포변화에 미치는 생맥산의 효과 신선호
  17. 동의생리학회지 v.15 no.5 생맥산이 대동맥 평활근 세포에서 NO생성에 미치는 영향 허윤
  18. J Cell Biol. v.152 no.1 The phagosome proteome. Insight into phagosome funtions Garin J;Diez R;Kieffer S;Dermine JF;Duclos E;Sadoul R;Rondeau C;Desjardins M
  19. Br Med Bull v.49 Free radicals and myocardial reperfusion injury Flitter WD
  20. Circulation v.80 Deleterious effects of oxygen radicals in ischemia/reperfusion. Resolved and unresolved issues Kloner RA;Przyklenk K;Whittaker P
  21. Cir Res. v.81 Death by design. Programmed cell death in cardiovascular biology and disease MacLellan WR;Schneider MD
  22. Endocrinology v.140 no.4 Signal transduction of arginine vasopressin-induced arachidonic acid release in H9c2 cardiac myoblasts role of Ca2+ and the protein kinase C-dependent activation of p42 mitogen-activated protein kinase Chen WC;Chen CC
  23. J Neurosci v.7 Glutamate neurotoxicity in cortical cell culture Choi DW;Maulucci-Gedde MA;Kriegstein AR
  24. Annu Rev Neurosci. v.21 Zinc and brain injury Choi DW;Koh JY
  25. J Pineal Res. v.26 Zinc accumulation in-adriamycin-induced cardiomyopathy in rats. effects of melatonin. a cardioprotective antioxidant. Morishima I;Okumura K;Matsui H;Kaneko S;Namaguchi Y;Kawakami K;Mokuno S;Hayakawa M;Toki Y;Ito T;Hayakawa
  26. 원광대학교 석사학위논문 실소산 전탕액이 배양 심근세포에 미치는 영향 한동훈
  27. 원광대학교 석사학위논문 구향충 전탕액이 배양 심근세포에 미치는 영향 조현익
  28. 동의생리학회지 v.14 no.2 자감초탕이 배양 심근세포에 미치는 영향 이래춘
  29. 본초강목 이시진
  30. 본초학 강병수(외)
  31. 임상본초학 신민교
  32. 본초방제학연구 신민교(외)
  33. 중약대사전 김창민(외 완역)
  34. 향약대사전 정보섭(외)
  35. 한약의 약리 김형균(외 편역)
  36. 대한해부학회지 v.33 no.6 H9c2 심장근육세포에 미치는 아연 세포독성의 기전 박찬희;소홍섭;최현준;김영희;오재민;최민규;정연태;박래길
  37. J Mol Cell Cardiol v.30 Oxidative Stress Induces DNA Fragmentation and Caspase Activation Via the c-Jun NH2-terminal Kinase Pathway in H9c2 Cardiac Muscle Cells Neil A.Turner;Fen Xia;Gohar Azhar;Xiaomin Zhang;Lixin Liu;Jeanne Y. Wei
  38. B J Biol, Chem. v.274 Regulation of FasLigand expression during activation-induced cell death in T-lymphocytes via nuclear factor Kasibhatla S;Genestier L;Green R
  39. Eur J Biochem. v.254 Apoptosis signal by death receptors Klaus SO;Davide F
  40. Genes Dev. v.13 Bcl-2 family members and the mitochondria in apoptosis Gross A;McDonnell JM;Korsmeyer SJ
  41. J Neurosci. v.48 A mechanism for glutamate toxicity in the C6 glioma cells involving inhibition of cystine uptake leading to glutathion depletion Kato S;K Negishi;K Mawatari;CH Kuo
  42. Proteomics. v.1 no.6 Analysis by matrix assisted laser desorption/ionisation-time of flight mass spectrometry of the post-translational modifications of alpha 1-antitrypsin isoforms separated by two-dimensional polyacrylamide gel electrophoresis Mills PB;Mills K;Johnson AW;Clayton PT;Winchester BG
  43. Proteomics v.1 no.10 New approaches to proteomic analysis of breast cancer Wulfkuhle JD;McLean KC;Paweletz CP;Sgroi DC;Trock BJ;Steeg PS;Petricoin EF 3rd