The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Ginseng radix on Alcohol-induced Decrease in New Cell Formation and Nitric Oxide Synthase Expression in Dentate Gyrus of Rats

인삼이 흰쥐의 치상회에서 알코올에 의한 새로운 신경세포 생성 및 nitric oxide synthase 발현에 미치는 영향

  • Min-Chul, Shin (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Ee-Hwa, Kim (Departments of Meridianology, College of Oriental Medicine, Semyung University) ;
  • Youn-Hee, Kim (Departments of Biochemistry, College of Oriental Medicine, Semyung University)
  • Published : 2002.09.01
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Abstract

목적 : 본 연구에서는 알코올 독성에 대하여 흰쥐의 치상회에서 새로운 신경세포의 생성 및 nitric oxide synthase (NOS) 발현에 인삼이 미치는 영향을 5-bromo-2-deoxyuridine (BrdU) 면역 조직 화학법 및 nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) 조직화학법을 통해서 관찰하고자 한다. 방법 : 실험동물을 정상군, 인삼처치군, 알코올처치군 및 알코올-인삼 처치군으로 분류하여 각각의 실험군에 3일간 BrdU (50mg/kg)를 복강주사하였다. 인삼처치군은 30mg/kg 용량의 인삼 전탕액을 중완혈에 약침주사하였고, 알코올 처치군은 2 g/kg 용량의 알코올을 투여하였으며. 알코올-인삼 처치군은 2 g/kg 용량의 알코올 및 30mg/kg 용량의 인삼 전탕액을 투여한 후 각각의 BrdU 양성 세포수와 NADPH-d 양성세포수를 관찰하였다. 결과 : 알코올 투여군은 BrdU 양성세포 및 NADPH-d 양성세포 발현이 감소하였으나, 인삼 및 알코올 인삼처치군에서는 알코올 투여군에 비해서 모두 증가하였다. 결론 : 인삼은 알코올에 의해서 유발된 새로운 신경세포 생성의 감소에 대하여 보호효과가 있으며, 알코올에 의해서 부가적으로 영향 받는 산화질소는 세포생성 조절에 중요한 역할을 하는 것으로 사려된다.

Keywords

References

  1. Biochem. Pharmacol. v.54 Panax ginseng pharmacology: a nitricoxide link? Grillis CN.
  2. J. Ethnopharmacol. v.22 Proliferative effect of ginseng saponin on neurite extention of primary cultured neurons of the rat cerebral cortex. Sugaya A;Yuzurihara M;Tsuda T;Yasuda K;.Kajiwara K;Sugaya E.
  3. Free Rad. Biol. Med. v.20 Ginseng extract scavenges hydroxylradical and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Zhang D;Yasuda T;Yu Y;Zheng P;Kawabata T:Ma Y;Okada S.
  4. Clin. Exp.Pharmacol. Physiol. v.14 Effects of Panax ginseng on blood alcohol clearance in man. Lee FC;Ko JH;Park JK;Lee JS.
  5. Neurotoxicol. Teratol. v.19 Ethanol-induced cell death in cultured rat astroglia. Holownia A;Ledig M;Menez JF.
  6. Dev. Brain Res. v.119 Glial-derived neurotrophic factor (GDNF) prevents ethanol-inducedapoptosis and JUN kinase phosphorylation. McAlhany Jr RE;West JR;Miranda RC.
  7. Science. v.287 Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome. Ikonomidou C;Bittigau P;Ishimaru MJ;Wozniak DF;Koch C;Genz K;Price MT; Stefovska V;Horster F;Tenkova T;Dikranian K;Oney JW.
  8. Behav.Brain Res. v.15 Memory deficits subsequent to chronic consumption of alcohol in mice: an analysis based on spontaneous alteration behavior. Beracochea D;Jaffard R.
  9. Trends Neurosci. v.16 The hippocampus and spatial memory. Squire LR.
  10. Nat.Med. v.4 Neurogenesis in the adult human hippocampus. Eriksson PS;Perfilieva E;Bjork-Eriksson T;Alborn AM;Nordborg C;Peterson DA; Gage FH.
  11. Eur. J. Neurosci. v.12 In vivo neurogenesis in the adult brain: regulation and functional implications. Fuchs E;Gould E.
  12. Neuropsychopharmacology. v.2999 no.21 Serotonin and hippocampal neurogenesis. Gould E.
  13. Neurosci. Lett. v.297 Acupuncture increases coll proliferation in dentate gyrus after transient global in gerbis. Kim EH;Kim YJ;Lee H;Huh Y;Chung JH;Seo JC;Kang JE;Lee HJ;Yim SV;Kim CJ.
  14. J. Neurosci. v.19 Estrogen stimulates a transient increase in the number of new neurons in the dentate gyrus of the adult female rat. Tanapat p;Hasings NB;Reeves AJ;Gould E
  15. Ann. Rev. Physiol. v.57 Nitric oxide signaling in the central nervous system. Garthwaite J;Boulton CL.
  16. Brain Res. v.445 Differential sensitivity of neuropeptide Y. Somatostatin and NADPH-diaphorasecontaining neurons in rat cortex and striatum to quinolinic acid. Boegman RJ;Parent A.
  17. Eur.J. Pharmacol. v.224 Ethanol can inhibit nitricoxide production. Persson MG;Gustafsson LE.
  18. Neurosci. Lett. v.204 Neuronal nitric oxide synthase expression in neuronal cell differentiation. Ogura T;Nakayama K;Fujisawa H;Esumi H.
  19. Brain Res. v.869 Morphological bases for a role of nitric oxide in adult neurogenesis. Moreno-Lopez B;Noval JA;Gonzalez-Bonet LG;Estrada C.
  20. J.Hepatol. v.20 Chronic ethanol feedirg increases apoptosis and cell proliferation in rat liver. Baroni GS;Marucci L;Benedetti A;Mancini R;Jezequel AM;Orlandi F.
  21. Alcohol. Clin. Exp. Res. v.17 Ethanol increases apoptotic cell death of thymocytes in vitro. Ewald SJ;Shao H.
  22. Science. v.260 Nitric oxide and carbon monoxide produce activity-dependent long-term synaptic enhancement in hippocampus. Zhuo M;Small SA;Kandel ÈR;Hawkins RD.
  23. Learn. Mem. v.2 7-Nitro indazole, a selective neuronal nitric oxide synthase inhibitor in vivo, impairs spatial learning in the rat. Holscher C;McGlinchey L;Anwyl R;Rowan MJ.
  24. Fitoterapia. v.71 Potential use of medicinal plants in the treatment of alcoholism. Carai MAM;Roberta A;Bombardelli E;Bourov I;Gessa GL;Lobina C;Morazzoni P; Pani M;Reali R;Vacca G;Colombo G.
  25. Planta Med. v.59 Effect of ginseng on plasma levels of ethanol in the rat. Lee YJ;Pantuck CB;Pantuck EJ.