Effect of Insulin-like Action and Insulin Sensitizing on 3T3-Ll Adipocytes from Coicis Semen

의이인이 3T3-Ll Adipocytes에서 인슐린성 작용과 인슐린 민감성에 미치는 영향

  • 김종욱 (우석대학교 한의과대학) ;
  • 최용휴 (우석대학교 한의과대학) ;
  • 주영승 (우석대학교 한의과대학) ;
  • 박선민 (호서대학교 식품영양학과) ;
  • 이미영 (한국한의학연구원 검사사업부) ;
  • 김호경 (한국한의학연구원 검사사업부) ;
  • 김홍준 (한국한의학연구원 검사사업부) ;
  • 고병섭 (한국한의학연구원 검사사업부)
  • Published : 2002.03.01

Abstract

Objectives : In this study, water extracts from Coix lachryma-jobi Linne var. mayuen Stapf. were investigated for their effects on insulin-like action and glucose uptake in 3T3-Ll cells. Methods : We examined the effects of insulin-like action on the differentiation of 3T3-Ll fibroblasts. The Coicis Semen was treated with hot water and the extract was freeze-dried. The hot water extract was chromatographed on nonionic polymer resin (Amberlite XAD-4, Sigma) with distilled water (Fr. 1), 20% (Fr. 2), 40% (Fr. 3), 60% (Fr. 4), 80% (Fr. 5), and 100% EtOH (Fr. 6), successively. Results : Total extract of Coicis Semen was fractionated into 0 to 100% MeOH with Amberlite XDA-4 column. Treatment of cells with $10{\;}\mu\textrm{g}/ml$ of total extracts of Coicis Semen significantly increased the differentiation (p<0.05). At $1{\;}\mu\textrm{g}/ml$ level of insulin, the differentiation was accelerated (p<0.01). The effect of extracts plus insulin on the differentiation was greater than that of insulin alone. In 3T3-Ll adipocytes, glucose uptake was higher by 2.7 times with 5 uM of total extract in low dosage of insulin (3 ng/ml) than without total extract. 5 and 50 uM of water and 40% MeOH fractions increased glucose uptake by 3.5 times in 3T3-Ll adipocytes (p<0.00l). Conclusions : Coicis Semen contains compounds which playa role of insulin-like action and insulin sensitizer.

Keywords

References

  1. 1992년 의료보험 통계 연보 의료보험 연합회
  2. 당뇨병 v.16 한국인 당뇨병의 임상적 특성 민헌기
  3. 한국영양학회지 v.30 하늘타리 재분획물이 streptozotocin 유발 당뇨 흰쥐의 인슐린 활성에 미치는 영향 및 급성 독성에 관한 연구 임숙자;최성숙
  4. 한국영양학회지 v.28 한국산 야생식용식물이 당뇨유발 흰쥐의 혈당 및 간과 근육의 에너지원 조성에 미치는 영향 임숙자;김수연;이주원
  5. 한국 잠사학회 v.39 고탄수화물 식이투여 마우스에서 누에 추출물이 소장내의${\alpha}$-glucosidase 활성에 미치는 영향 정성현;김미선;류강선
  6. 本草學 전국한의과대학본초학교수(공편)
  7. 임상본초학개정증보판 신민교
  8. 和漢藥百科圖鑑 全改訂新版 難波恒雄
  9. Biol. Pharm. Bull. v.22 no.10 Isolation and antihyperglycemic activity of bakuchiol from Otholo-bium pubsecens(Fabaceae), a peruvian medicinal plant used for the treatment of diabetes Krenisky JM;Luo J;Carney JR
  10. Gaxiong Yi Xue Ke Xue Za Zhi. v.16 no.9 Effect of crude drugs on glucose uptake in 3T3-L1 adipocyte Hong SJ;Fong JC;Hwang JH
  11. J. Traditional Med. v.13 Insulin like action of trans-10-hydroxy-2-decanoic acid and its related substance Kameda K;Chikaki M;Morimoto C;Jiang M;Okuda H
  12. J. Traditional Med. v.14 Isolation of Insulin like substance from Ephedra sinica STAPF Takaku T;Jiang M;Okuda H;Maeda N.
  13. J. Traditional Med. v.14 Effect of Jasmin Tea on obesemice Han L-K;Li J. Morimoto C;Takaku T;Okuda H
  14. Diabetes. v.50 no.4 A nove small molecule that directly sensitizes the insulin receptor in vitro and in vivo Manchem VP;Goldfine ID;Kohanski RA;Cristobal CP;Lum RT;Schow SR;Shi S;Spevak WR;Laborde E;Toavs DK;Villar HO;Wick MM;Kozlowski MR
  15. Mol. Endocrinol. v.14 no.9 A selective peroxisome proliferator-activated receptor- gamma (PPARgamma) modulator blocks adipocyte differentiation but stimulates glucose uptake in 3T3-L1 adipocytes Mukherjee R;Hoener PA;Jow L;Bilakovics J;Klausing K;Mais DE;Faulkner A;Croston GE;Paterniti JR Jr.
  16. Br. J. Nutr. v.81 no.3 Insulin-reasing and Insulin-like activity of the Traditionnal antidiabetic plant Corian-durm sativum(coriander) Gray AM;Flatt PR
  17. Endocrinology v.137 Troglitazone enhances differentiation, basal glucose uptake, and GLUT1 protein levels in 3T3-L1 adipocytes Tafuri SR