Comparison of Inoculation Effects for Different Seed and Nodule Sources in Alnus firma Grown in Yeosu Industrial Complex(I)

여수산업단지에서 자라는 사방오리나무에서 채취한 종자와 질소고정 뿌리혹의 접종 효과 비교(I)

  • Su-Young Woo (Department of Forest Resources and Environment, College of Life Science & Natural Resources, Sangju National University) ;
  • Oh-Kyu Kwon (Department of Forest Resources and Environment, College of Life Science & Natural Resources, Sangju National University)
  • Published : 2002.03.01

Abstract

This study identified the physiological characteristics of Alnus firma seedlings collected from air polluted industrial complex of Yeosu area with inoculation of nitrogen-fixing nodule. A. firma individuals resistant or sensitive to air pollution stress at the study area were selected. Seeds of the individuals were sown in a pot and inoculated with the nodules collected at the same area about 3 weeks after germination. To compare the inoculation effects, photosynthesis was measured among the A. firma seedlings under 12 inoculation combinations of seed sources and nodule sources. Photosynthetic activities of seedlings from resistant mother trees were obviously higher than those from sensitive mother trees. In general, the seedlings inoculated with nodule of sensitive trees, showed a worse physiological trend on every combination. In this study, inoculation effect was less important factor than seed sources to photosynthetic activities of A. firma. This physiological characteristic seemed to be determined by seed sources rather than nodule sources.

여수산업단지 같은 대기오염지역에서 채취한 사방오리나무의 질소고정 뿌리혹의 접종 효과를 보기 위해서 오염에 대해서 저항성 개체와 민감한 개체를 선발하고 종자를 채취한 후 파종을 하고 발아한 후 3주 후의 유묘를 같은 지역에서 채취한 뿌리혹으로 12개의 조합으로 접종을 하였다. 접종효과를 비교하기 위해서 광합성 능력을 측정하였다. 대기오염에 대해 저항성을 보이는 모수에서 채취한 종자가 민감한 개체에서 채취한 것보다 광합성 능력이 높은 경향을 나타냈다. 대기오염에 민감한 모수에서 채취한 뿌리혹으로 접종한 조합이 일반적으로 생리활성이 낮았다. 그러나 접종효과는 뿌리혹을 채취한 모수보다는 종자를 채취한 모수가 어떤 상태인가에 따라서 광합성 능력이 더 영향을 많이 받는 것으로 나타났다. 그렇기 때문에 생리적인 특성에 대한 영향은 뿌리혹을 채취한 모수보다는 종자의 모수가 더 중요한 요인 같다.

Keywords

References

  1. Bergersen, F.J. 1982: Root Nodules of Legumes: Structure and Functions. John Wiley and Sons Ltd. New york. 164 p.
  2. Binkley, D., J.D. Lousier and K. Cromack Jr. 1984: Effects of Alder in a Douglas-fir plantation. Forest Science, 30(1), 26-35.
  3. Burggraaf, A.J.P., J. Linden and T. Tak. 1983: Studies on the localization of infectible cells on Alnus glutinosa roots. Plant and Soil, 74, 175-188.
  4. Cha, D.H. and D.K. Lee. 1996: Effects of different aluminum levels on growth and root anatomy of Alnus hirsuta Rupr. Seedlings. J. Sustainable Forestry, 3(2), 45-63.
  5. Ghim, Y.S., D.Y. Yoon, Y.S. Chang and K.C. Moon. 1999: Offsite consequence analysis for accidental release scenarios of toxic substances in the Yochon Area. J. of Korean Society for Atmospheric Environment, 15(2), 151-158.
  6. Houston, D.B. and G.R. Stairs. 1973: Genetic control of sulfur dioxide and ozone tolerance in Eastern White Pine. Forest Science, 19, 267-271.
  7. Kozlowsi, T.T. 1972: Seed Biology I. Academic Press. New York. 416p.
  8. Lee, S.W., S.Y. Woo, Y.B. Koo and S.K. Lee. 1998: Genetic differences between the tolerant and the sensitive trees in an air polluted Prunus sargentii stand J. of Kor. For. Soc., 87(1), 74-81.
  9. Lim, J.H. 1988: Effectivity of Frankia avc I1 on four Alnus species, their nodule morphology and nitrogen fixation. MS thesis, Seoul National Univ. 29 p.
  10. Licor Inc. 1999: Using the Li-6400, Manual Series I (2nd Edition). Licor Inc., Nebraska, 150 p.
  11. Reich, P.B. 1987: Quantifying plant response to ozone: A unifying theory. Tree Physiology, 3, 63-91.
  12. Sheng, Y., G.K. Podila and D.F. Karnosky. 1997: Differences in $O_3$-induced Superoxide Dismutase and Glutathione Antioxidant Expression in $O_3$ Tolerant and Sensitive Trembling Aspen(Populus tremuloides Michx.) Clones. Forest Genetics, 4(1), 25-33.
  13. Taylor, G.E. Jr. and D.W. Johnson. 1994: Air pollution and forest ecosystems: a regional to global perspective. Ecological Applications, 4, 662-689.
  14. Woo, S.Y. and D.K. Lee. 1999: Antioxidant enzyme activities to air pollution of Alnus firma in Yochon Industrial Complex. J. of Kor. For. Soc., 88(2), 221-228.