한국수의병리학회지 (Korean Journal of Veterinary Pathology)
- 제6권1호
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- Pages.11-18
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- 2002
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- 1226-766X(pISSN)
항혈청 투여에 따른 돼지 전염성 위장염 예방효과 I. 혈청학적 결과, RT-PCR 검사, 형광항체검사
Preventive Effects on Transmissible Gastroenteritis(TGE) Using by TGEV Antiserum I. Serological Results, RT-PCR for Fecal and Small Intestin, FA Test
- 지영철 (강원대학교 수의학과) ;
- 한정희 (강원대학교 수의학과) ;
- 권혁무 (강원대학교 수의학과) ;
-
한태욱
(강원대학교 수의학과) ;
- 정현규 (도드람 양돈조합) ;
-
박봉균
(서울대학교 수의과대학, 농생명공학부)
- Chi, Yong-Zhe (Department of Veterinary Medicine, Kangwon National University) ;
- Han, Jeong-Hee (Department of Veterinary Medicine, Kangwon National University) ;
- Kwon, Hyuk-Moo (Department of Veterinary Medicine, Kangwon National University) ;
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Hahn, Tae-Wook
(Department of Veterinary Medicine, Kangwon National University) ;
- Jeong, Hyun-Kyu (Dondram Pig Farmers' Cooperative) ;
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Park, Bong-Kyun
(College of Veterinary Medicine and School of Agricuultural Biotechnology, Seoul National University)
- 발행 : 2002.06.01
초록
The purpose of this study was to investigate to potective effects against transmissible gastyoenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 5 hrs, 24hrs and 36hrs after birth. five piglets administered the antiserum were experimentally infected with TGEV at four-day-old. Control group were four piglets infected with TGEV only. Serum antibody titers against TGEV were examined by serum neutralization(SN) test, dectection for TGEV or TGEV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the TGEV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of TGEV in feces and small intestines by RT- PCR were 24.5% and 20.0% in TGEV antiserum treated group and 44.0% and 75.0% in control group, respectively. 3 The detection rate of TGEV antigen in the small intestine by IFA were 26.7% in TGEV antiserum treated group and 75.0% in control group, respectively. It was concluded that oral administration of antiserum against TGEV to piglets was effective in preventing TGEV infection.