Journal of Photoscience
- Volume 9 Issue 2
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- Pages.314-316
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- 2002
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- 1225-8555(pISSN)
Association between a M-Iacking mutant D75N of pharaonis phoborhodopsin and its transducer is stronger than the complex of the wild-type pigment: Implication of the signal transduction
- Sudo, Yuki (Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University) ;
- Iwamoto, Masayuki (Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University) ;
- Shimono, Kazumi (Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University) ;
- Kamo, Naoki (Laboratory of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University)
- Published : 2002.08.01
Abstract
In halobacterial membrane, pharaonis phoborhodopsin (or pharaonis sensory rhdopsin II, psRII) forms a complex with its transducer pHtrII. Flash-photolyis of D75N mutant did not yield M-intermediate but an O-like intermediate is observed. We examined the interaction between D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of n-dodecyl-
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