Journal of Photoscience
- 제9권2호
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- Pages.278-280
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- 2002
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- 1225-8555(pISSN)
G-Proteins Expressed in the Ocellus of the Hydromedusan, Spirocodon saltatrix.
- Iwasa, Tatsuo (Department of Material Science and Engineering, Muroran Institute of Technology,) ;
- Shimazaki, Yumiko (Ushimado Marine Laboratory, Faculty of Science, Okayama University) ;
- Yamamoto, Masamichi (Ushimado Marine Laboratory, Faculty of Science, Okayama University) ;
- Ohtsu, Kohzoh (0ki Marine Biological Station, Department of Biological Science, Shimane University)
- 발행 : 2002.08.01
초록
We have cloned a hydromedusan opsin cDNA and showed that the deduced amino acid sequence of the cytoplasmic loop between helices 5 and 6 (loop 5-6) was clearly different from that reported so far. The amino acid sequence of the loop 5-6 is important on determination of the specificity for the coupled G- protein. To clarify which class of G-protein mediates the phototransduction system in the ocellus of the hydromedusan, we investigated G-proteins expressed in the ocellus. By PCR against the cDNA of the ocellus with primers designed according to the conserved amino acid sequence in G-protein a subunit, we obtained three kinds of cDNA fragments. Based on the sequence similarities, ttwo of them (JGI and JG3) were classified as
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