Journal of Photoscience
- Volume 9 Issue 2
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- Pages.126-129
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- 2002
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- 1225-8555(pISSN)
Structure and Photoreaction of Photoactive Yellow Protein
- Imamoto, Yasushi (Graduate School of Materials Science, Nara Institute of Science and Technology) ;
- Harigai, Miki (Graduate School of Materials Science, Nara Institute of Science and Technology) ;
- Shimizu, Nobutaka (Graduate School of Materials Science, Nara Institute of Science and Technology) ;
- Kamikubo, Hironari (Graduate School of Materials Science, Nara Institute of Science and Technology) ;
- Yamazaki, Yoichi (Graduate School of Materials Science, Nara Institute of Science and Technology) ;
- Kataoka, Mikio (Graduate School of Materials Science, Nara Institute of Science and Technology)
- Published : 2002.08.01
Abstract
The chromophore/protein interactions in the photocycle intermediates of photoactive yel- low protein (PYP) were probed by site-directed mutagenesis. The absorption spectra of L- intermediates produced from E46Q, T50V, and R52Q mutants were calculated using the absorption spectra of dark states and difference absorption spectra between L-intermediates and dark states, and compared with that of PYP