Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Pharmacological Activities of a New Glycosaminoglycan, Acharan Sulfate Isolated from the Giant African Snail Achatina fulica

  • Shim, Jin-Young (Natural Products, Research Institute, Seoul National University) ;
  • Lee, Yeon-Sil (Natural Products, Research Institute, Seoul National University) ;
  • Jung, Sang-Hoon (Natural Products, Research Institute, Seoul National University) ;
  • Choi, Hyung-Seok (Natural Products, Research Institute, Seoul National University) ;
  • Shin, Kuk-Hyun (Natural Products, Research Institute, Seoul National University) ;
  • Kim, Yeong-Shik (Natural Products, Research Institute, Seoul National University)
  • Published : 2002.12.01
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Abstract

Acharan sulfate (AS) is a glycosaminoglycan (GAG) prepared from the giant African snail, Achatina fulica. In this study, some biological activities of AS were evaluated on the basis of structural similarities to heparin/heparan sulfate and the biological functions of GAGs. We demonstrated that it exhibited strong immunostimulating activities as measured by carbon clearance test in mice and in vivo phagocytosis. It also exhibited a significant hypoglycemic activity in epinephrine (EP)-induced hyperglycemia as well as antifatigue effects by weight-loaded forced swimming test. And it showed hypolipidemic activities in cholesterol-rich mixture induced hyperlipidemia in rats. The above results indicate that AS has diverse biological activities and suggest therapeutically important target molecules.

Keywords

References

  1. Jeong, J. A., Toida, T., Linhardt, R., and Kim, Y. S., Localization and characterization of acharan sulfate in the body of the giant African snail Achatina fulica. Compo Biochem. Physiol., 130, 513-519 (2001) https://doi.org/10.1016/S1096-4959(01)00468-7
  2. Johnson, J. A., and Fusaro, R. M, The quantitative enzymic determination of animal liver glycogen. Anal. Biochem., 15, 140-149 (1966) https://doi.org/10.1016/0003-2697(66)90256-9
  3. Kreisman, S. H., Ah Mew N, Arsenault, M., Nessim S. J., Halter J. B., Vranic, M., and Marliss E. B., Epinephrine infusion during moderate intensity exercise increases glucose production and uptake. Am. J. Physiol. Endocrinol. Metab., 278, E949-957 (2000) https://doi.org/10.1152/ajpendo.2000.278.5.E949
  4. Kimura, Y., Kubo, M., Tani, T., Arichi, S., Ohminami, H., and Okuda, H., Studies on Scutellariae radix. III. Effects on lipid metabolism in serum, liver and fat cells of rats. Chem. Pharm. Bull., 29, 2308-2312 (1981) https://doi.org/10.1248/cpb.29.2308
  5. Kim, Y. S., Jo, Y. Y., Chang, I. M., Toida, T., Park Y. M., and Linhardt, R. J., A new glycosaminoglycan from the giant African snail Achatina fulica. J. Biol. Chem., 271, 11750-11755 (1996) https://doi.org/10.1074/jbc.271.20.11750
  6. Linhardt, R. J., and Toida, T., Heparin oligosaccharides: New analogues, development and applications. In Carbohydrates as drugs, In Witczak, Z. J., and Nieforth. Maroel Dekker, K. A. (Eds.). New York, pp. 277-341, (1997)
  7. Marcil, A., Harcus D, Thomas, D. Y., and Whiteway, M.. Candida albicans killing by RAW 264.7 mouse macrophage cells: effects of Candida genotype, infection ratios, and gamma interferon treatment. Infect. lmmun., 70, 6319-6329 (2002) https://doi.org/10.1128/IAI.70.11.6319-6329.2002
  8. Mariam, A., Asmawi, M. Z., and Sadikun., Hypoglycemic activity of the aqueous extract of Orthosiphon stamineus. Fitoterapia, 57, 465-468 (1996)
  9. Moriura, T., Matsuda, H., and Kubo, M., Pharmacological study on Agkistrodon blomhoffii blomhoffii BOlE. V. Anti-fatigue effect of the 50% ethanol extract in acute weight-loaded forced swimming-treated rats. Biol Pharm. Bull., 19, 62-66 (1996) https://doi.org/10.1248/bpb.19.62
  10. Wagner, H., Proksch, A., Riess-Maurer, I., Vollmar, A, Odenthal, S., Stuppner, H., Jurcic, K., Le Turdu, M., and Heur, Y. H., Immunostimulant action of polysaccharides (heteroglycans) from higher plants. Preliminary communication. Arzneimittelforschung, 34, 659-661 (1984)