YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Immune Stimulating Efficacy of Insoluble $\beta$-l, 3-glucan from Agrobacterium sp. R259 KCTC 10197BP

Agrobacterium sp. R259 KCTC 10197BP로부터 생산된 $\beta$-1, 3-glucan의 면역 활성 효능

  • 심정현 (한국생명공학연구원 세포생물학연구실) ;
  • 최원아 ((주)더멋진 바이오텍) ;
  • 상병찬 (충남대학교 낙농학과) ;
  • 윤도영 (한국생명공학연구원 세포생물학연구실)
  • Published : 2002.12.01
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Abstract

$\beta$-l, 3-glucans are well known to enhance the immune reactions, resulting in antitumor, antibacterial, antiviral, anticoagulatory and wound healing activities. $\beta$-1, 3-glucans have various activities depending on molecular weight, degree of branching, conformation, water-solubility and intermolecular association. However, the $\beta$-1, 3-glucans linked backbone structure is essential and $\beta$-D-glucopyranosyl units are required for immunopotentiating activities. In this study, we tested the immunophamacological activities of insoluble $\beta$-1, 3-glucan from Agrobacterium sp. R259 KCTC 10197BP and confirmed the following activities: (1) IFN-${\gamma}$ production in PBMCs in the presence or in the absence of PHA, LPS, IL-18, and IL-12; (2) the induction of various cytokines in the spleen and thymus; (3) the adjuvant effect on the antibody production; (4) the cytotoxic and antitumor effects on cell lines and ICR mice. These results strongly suggest that $\beta$-1, 3-glucan from Agrobacterium sp. R259 KCTC 10197BP possesses various immunopharmacologica1 activities.

Keywords

References

  1. Bohn, J. A. and Bemiller, J. N. : (1$\rightarrow$3)-$\beta$-glucan as biological response modifier : a review of structure-functional activity relationships. Carbohydrate Polymers. 28, 3 (1995)
  2. Hamada, H., Sato, M., Ozawa, M., Ohi, Y. and Muramatsu, T. : Transmembrane control of laminin, lectin receptors and surface villi in teratocarcinoma-derived endodermal cells. Exp. Cell Res. 154, 299 (1984) https://doi.org/10.1016/0014-4827(84)90689-X
  3. Jamas, S., Easson, D., J., Ostroff, G. R. and Onderdonk, A. B. : PGG-glucans. A novel class of Macrophage-activating Immunomodulators. Acs Symp. Ser. 469, 44 (1991)
  4. Williams, D. L. : Overview of (1$\rightarrow$3)-$\beta$-D-glucan immunobiology. Mediators of Inflammation. 6, 247 (1997) https://doi.org/10.1080/09629359791550
  5. Doita, M., Rasmussen, L. T., SeljeIid, R. and Lisky, P. E. : Effects of soluble aminated $\beta$-1, 3-D-polyglucose on human monocytes: stimulation of cytokine and prostaglandin E2 production but not antigen-presenting function. J. Leukocyte Biology. 49, 342 (1991)
  6. Artursson, P., Edman, P. and Ericsson, J. L. E. : Macrophage stimulation with some structurally related polysaccharides. Scand. J. Immunol. 25, 245 (1987) https://doi.org/10.1111/j.1365-3083.1987.tb01070.x
  7. Petersen, R. D., Reinhold, W. and Tyborczyk, J. : Cytokines in cosmetology. Cosmetics and Toiletries Magazine. 112, 165 (1997)
  8. Kasai, S., Fujimoto, S., Kazuo, N., Baba, H. and Kunimoto, T. : Antitumor activity of polymorphonuclear leukocytes activated by a ${\beta}-1$, 3-D-Glucan. J. Pharmacobio. DYN. 14, 519(1991)
  9. Krause, J. and Franz, G. : Immunomodulating effects of polysaccharides from medicinal plants. Adv. Exp. Med. Biol. 319, 299 (1992)
  10. Miura, T., Niura, N. N., Ohno, N., Adach, I., Shimada, S. and Yadomae, T. : Failure in antitumor activity by overdise of an immunomodulating $\beta$-glucan preparation, sonifilan. Biol. Pharm. Bull. 23, 243 (2000)
  11. Miura, T., Ohno, N., Minra, N. N., Shimada, S. and Yadomae, T. : Inactivation of a particle $\beta$-glucan by proteins in plasma and serum. Biol. Pharm. Bull. 20, 1103 (1997)
  12. Ohno, N., Niura, N. N., Chiba, N., Adachi, Y. and Yadomae, T. : Comparison of the immunopharmacological activities of triple and single-helical schizophyllan in mice. Biol. Pharm. Bull. 18, 1242 (1995) https://doi.org/10.1248/bpb.18.1242
  13. Wagner, H., Stuppner, H., Schafer, W. and Zenk, M. : Immunologically active polysaccharides of Echinacea purpura cell cultures. Phytochemistry. 27, 119 (1988) https://doi.org/10.1016/0031-9422(88)80601-0
  14. Adrian, J., Puren, G., Fantuzzi and Dinarello, C. A. : Gene expression, synthesis, and secretion of interleukin 18 and Interleukin 1 are differentially reg ulated in human blood ononuclear cells and mouse spleen cells. Proc. Natl. Acad. Sci. 96, 2256 (1999) https://doi.org/10.1073/pnas.96.5.2256
  15. Chang, S. T. and Miles, P. G. : Historical record of the early cultivation of lentinus in china. The Mushroom J. 7, 31 (1987)
  16. Chomczynski, P. and Sacchi, N. : Single-step method of RNA isolation by acid guanidinium thyocyanate-phenol-chlorofom Extraction. Analytical Biochemistry. 162, 156 (1987)
  17. Colle, J. H., Pierre, B. F., Singer, M., Hevin, B. and Milon, G. : Quantitation of messenger RNA by competitive RT-PCR: a simplified read out assay. J. Immunological Method. 210, 175 (1997)
  18. Petitprez, K., Khalife, J., Cetre, C., Fontaine, J.. Lafitte, S., Capron, A. and Grzych, J. M. : Cytokine mRNA expression in lymphoid organs associated with the expression of IgA response in the rat. Scand. J. Immnol. 49, 14 (1999) https://doi.org/10.1046/j.1365-3083.1999.00461.x
  19. Vandebriel, R. J.. Spiekstra, S. W., Hudspith, B. N., Meredith, C. and Loveren, H. V. : In vitro exposure effects of cyclosporin A and bis (tri-n-butylin)oxide on lymphocyte proliferation, cytokine(receptor) mRNA expression, and cell surface marker expression in rat thymocytes and splenocytes. Toxicology. 135, 49 (1999) https://doi.org/10.1016/S0300-483X(99)00049-9
  20. Cetre, C., Cocude. C., Poerrot, C., Godin, C. and Capron, A. : In vivo expression of cytokine mRNA in rats infected with Schistosoma mansoni. Parasite Immunology. 20, 135 (1998). https://doi.org/10.1046/j.1365-3024.1998.00135.x
  21. Cho, Y. C., Cho, O., Lee, K., Park, S. and Yoon, D. : Development of screening systems for drugs against human papillomavirus-associated cervical cancer: based on E6-E6AP binding. Antiviral Res. 47, 199 (2000)
  22. Raerz, C. R. : Biochemistry of endotoxins. Annu. Rev. Biochem. 59, 129 (1990) https://doi.org/10.1146/annurev.bi.59.070190.001021
  23. Ferris, F. G. and Beveridge, T. J.: Site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide. Can. J. Microbiol. 32, 52(1986)
  24. Labis, H., Barnickel, H. B., Naumann, E. T. R. and Giesbrecht, P. : High state of order of isolated bacterial lipopolysaccharide and its possible contribution to the permeation barrier property of the outer membrane. J. Bacteriol. 162, 9 (1985)
  25. Lam, J. S., Graham, J. L. Dasgupta, T. and Beveridge, T. J, : Ultrastructural examination of the lipopolysaccharides of pseudomonas aeruginosa strains and their isogenic rough mutants by freeze-substitution. J. Bacteriol. 174, 7159 (1992)
  26. Goldsby, R. A., Kindt, T. J. and Osborne, B. A. : Kuby Immunology 4th ed., W. H. Freeman and Company, New York, p, 303 (2000)