YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Protective Effect of R. palmatum on 1-Methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP)-induced Neurotoxicity in Mice

생쥐의 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-유도 신경독성에 대한 대황의 보호효과

  • 이형철 (원광대학교 한의과대학 병리학교실) ;
  • 김대근 (원광대학교 한의과대학 병리학교실) ;
  • 조원준 (원광대학교 한의과대학 병리학교실) ;
  • 황석연 (충북대학교병원 임상병리과) ;
  • 이영구 (KT＆G중앙연구원 생화학실) ;
  • 김명동 (상지대학교 한의과대학 생리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Published : 2002.12.01
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Abstract

The protective efficacy of Rheum palmatum water extract on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism was studied in C57BL/6 mice. In order to demonstrate neuroprotective effect of R. palmatum extract, animals were administered intraperitoneally with the water extract (100 or 200 mg/kg/day) for 14 days, and MPTP (10 mg/kg/day) was injected subcutaneously into the mice for the first 6 consecutive days from the beginning 1 hr before R. palmatum extract treatment. All animals were measured the several neurobiochemical markers such as dopamine level and monoamine oxidase B (MAO-B) activity in various regions of brain. The treatment of mice with R. palmatum extract was confirmed recovery effect on MAO-B activity in the cerebellum and the cerebral cortex. R. palmatum extract was attenuated the MPTP-induced depletion of substantia nigra dopamine. The contents of MDA, a marker of lipid peroxidation, in brain tissues (cerebellum and cerebral cortex mitochondria) were decreased significantly by R. palmatum extract. These results suggest that R. palmatum water extract plays an effective role in attenuating MPTP-induced neurotoxicity in mice. This protective effect of R. palmatum might be estimated the result from the inhibitory activity on monoamine oxidase B and the enhancement of antioxidant activity.

Keywords

References

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