Effects of Polymannuronate Feeding on Compositions of Serum and Liver Lipids in the High-Cholesterol Fed Rats

Polymannuronate의 급이가 고콜레스테롤 급이 흰쥐의 혈청 및 간 지질 조성에 미치는 영향

  • 이동수 ((주)케이비피 생물공학연구소) ;
  • 남택정 (부경대학교 식품생명공학부) ;
  • 최재수 (부경대학교 식품생명공학부) ;
  • 변재형 (부경대학교 식품생명공학부)
  • Published : 2002.08.01

Abstract

The cholesterol and fatty acid levels in serum and liver were compared in 4-week-old Sprague-Dawley male fed by addition of polymannuronate (M, 5.0%), polyguluronate (G, 5.0%), and polymannuronate and polyguluronate (MG, 2.5% to each) with by addition of cholesterol (1.0%). Feed efficiency by the addition of M, MG, and G was lower than cholesterol fed group (p<0.01). The liver weights were less in M, MG, and G fed groups than in cholesterol fed group (p<0.01). Triglyceride levels in serum and liver were 58.2∼77.4% and 51.5∼65.5% lower in M, G and MG fed groups than cholesterol fed group, respectively. Total-, LDL-, and free-cholesterol levels in serum and liver in M, MG, and G fed group were significantly lower than cholesterol fed group. The cholesterol levels were the most reduced in M fed group. However, HDL- cholesterol level in serum was increased in M, MG, and G fed group (p<0.01). The of polyene levels were 47% higher in serum and 76% in liver in M fed group than in cholesterol fed group. The activities of GOT and GPT were lower in M, MG, and G fed group than in cholesterol fed group (p<0.01). Above the results demonstrate that supplementation of low molecular polymannuronate in diets improve physiologically lipid composition in serum and liver.

Keywords

References

  1. Tsuji, K., Oshima, S., Matsuzaki, E., Nakamura, A., Innami, S., Tezuka, T. and Suzuki, S. : Effect of polysaccharides on cholesterol metabolism (part 1) Studies on konnyaku powder, sodium alginate and pectin. Eiyogaku zashi, 26(3), 113 (1968, in Japanese)
  2. Tsuji,K and Tsuji, E. : Effect of polysaccharides on cholesterol metabolism (part 3) Effect of several polysaccharides on serum cholesterol levels in cholesterol-fed rats. Eiyogaku zashi, 32(4), 155 (1974, in Japanese)
  3. Tsuji, E. and Tsuji, K. and Suzuki, S. : Effect of polysaccharides on cholesterol metabolism (part 6) Effect of various polysaccharides on serum and liver cholesterol levels in cholesterol-fed rats. Eiyogaku zashi, 33(6), 273 (1975, in Japanese)
  4. Wu, J and Peng, S. S. : Comparison of hypolipidemic effect of refined Konjac meal with several common dietary fibers and their mechanisms of action. Biomed. Environ. Sci., 10, 27 (1997)
  5. Ikegami, S., Tsuchihashi, F, Harada, H., Tsuchihashi, N., Nishide, E. and Innami, S. : Effect of viscous indigestible polysaccharides on pancreatic-billary secretion and digestive organs in rats. J. Nutr., 120, 353 (1990)
  6. Sandberg, A., Anderson, H., Bosaeus, I., Carlsson, N., Hasselblad, K. and Harrad, M. : Alginate, small bowel sterol excretion, and absorption of nutrients in ileostomy subjects, Am. J. Clin. Nutr., 60, 751 (1994)
  7. Kimura, T., Takahashi, K., Ueda, Y., Obika, H., Kobayashi, Y. and Tsuji, K. : Effects of the primary structure of alginate on fecal excretion of sodium in rats. Nippon Nogeikagaku Kaishi, 67(8), 1177 (1993)
  8. 이동수, 남택정, 변재형 : 저분자화 알긴산이 콜레스테롤식이 흰 쥐의 혈청과 간장지질의 콜레스테롤 수준 및 지방산 조성에 미치는 영항, 한국수산학회지 31(3), 399 (1998)
  9. Suzuki, T., Nakai, K., Yoshie, Y., Shirai, T. and Hirano, T. : Effects of sodium alginates rich in guluronic and mannuronic acids on cholesterol levels and digestive organs of highcholesterol- fed rats. Nippon Suisan Gakkaishi, 59(3), 545 (1993b)
  10. Lee, D. S., Lee, J. W., Pyeun, J. H. and Nam, T. J. : Isolation of polymannuronate of low molecular weight from alginate hydrolyzed by organic acids. J. of Food Biochem. in press.
  11. Bligh, E. G. and Dyer, W. J. : A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37(8), 911 (1959)
  12. Suzuki, T., Nakai, K, Yoshie, Y., Shirai, T. and Hirano, T. : Digestibility of dietary fiber in brown alga, kombu, by rats. Nippon Suisan Gakkaishi, 59(5), 878 (1993a)
  13. Oku, T., Konishi, F. and Hosoya, N. : Effect of various unavailable carbohydrates and administrating periods on several physiological functions of rats. Nutrition and Food, 34(5), 437 (1981, in Japanese)
  14. Venter, C. S., Vorster, H. H. and Van Der Nest, D. G. : Comparison between physiological effects of konjacglucomannan and propionate in babbons fed 'Western' diets.J. Nutr., 120, 1046 (1990)
  15. Tsai, A. C., Elias, J.. Kelley, J. J., Lin, R. C. and Robson, J. R. K : Influence of certain dietary fibers on serum and tissue cholesterol levels in rats. J. Nutr., 106, 118 (1976)
  16. Nicoll, A., MIller, N. E, and Lewis, B. : High density lipoportein metabolism. Adv. Lipid Res., 17, 53 (1980)
  17. Castelli, W. P., Garrison, R. J., Wilson, P. W. F., Abbott, R. D., Kalousdian S. and Kannel, W. B. : Incidence of coronary heart disease and lipoprotein cholesterol levels. JAMA., 256, 2835 (1986)
  18. Rizek. R. L., Welsh, S. O., Marston, R. M. and Jackson, E. M. : In dietary fats and health (Perkins, E. G. and W. J. Visek, eds.), American Oil Chemists Society. Champaign, 13 (1983)
  19. Jenkins, D. J. A., Gassull, M. A., Cocher; B. and Alberti, K G. : Decrease in postplandial insulin and glucose concentration by guar gum and pectin. Ann. Intern. Med., 86, 20 (1977)