Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of a Biosurfactant Mannosylerythritol Lipid by Resting Cell of Candida sp. SY16.

Candida sp. SY16의 휴식세포를 이용한 생물계면활성제 Mannosylerythritol Lipid의 생산

  • 김희식 (한국생명공학연구원 생물공정연구실) ;
  • 전종운 (한국생명공학연구원 생물공정연구실) ;
  • 최우영 (충남대학교 농화학과) ;
  • 오희목 (한국생명공학연구원 생물공정연구실) ;
  • 이기형 (공주대학교 생명과학과) ;
  • 권태종 (건국대학교 미생물공학과) ;
  • 윤병대 (한국생명공학연구원 생물공정연구실)
  • Published : 2002.06.01
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Abstract

The resting cells of Candida sp. SY16 produced a large amount of mannosylerythritol lipid as a biosurfactant when incubated in the distilled water containing only the carbon source. The resting cells exhibited the highest production at 20 g cells per liter on the soybean oil of 75 g/1 as a sole substrate and pH 4∼5 in the shaking culture. Under the optimal conditions, the biosurfactant was extracellularly produced to 58 g/1 after 120 h in jar fermentor, and the yield became higher than that obtained by using the glowing cells of the strain in batch fermentation.

Candida sp. SY16의 휴식세포를 이용하여 탄소원만 포함되 있는 증류수로부터 많은 양의 생물계면활성제(mannosylerythritol lipid: MEL)를 생산할 수 있었다. 플라스크를 이용한 생산반응의 경우, 20 g/l의 휴식세포를 75g/1의 soybean oil이 포함되있고, pH는 4~5범위에서 반응할 때, 가장 높은 생산수율을 얻을 수 있었으며, 높은 농도($PO_4$-P 농도 198 $\mu\textrm{g}$/ml 이상)의 인은 MEL 생산을 저해하는 것으로 나타났다. 최적화된 조건으로 jar fermentor를 이용하여 휴식세포를 반응한 경우 생물계면활성제를 120 h 반응 후, 58 g/l로 얻을 수 있었으며, 그 생산성은 성장세포를 이용한 회분식 발효의 경우보다 높았고, 배양시간도 단축시킬 수 있었다.

Keywords

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