한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제30권1호
  • /
  • Pages.33-38
  • /
  • 2002
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Bacillus sp. LM-8이 생산하는 Lactobacillus plantarum 용균 효소의 정제 및 효소 특성

Purification and Enzyme Property of a Cell-Wall Lytic Enzyme Produced by Bacillus sp. LM-8 against Lactobacillus plantarum.

  • 마호우 (강원대학교 바이오 산업공학부) ;
  • 신원철 (강원대학교 바이오 산업공학부)
  • 발행 : 2002.03.01
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초록

Lactobacillus plantarum 용균 효소를 생산하는 균주를 배양하여 생산된 효소를 정제한 결과 비활성도가 5.8 units/mg protein 이었고 정제도 8.3배, 수율은 30%이었다. 정제효소의 분자량은 gel filtration과 SDS-polyacrylamide gel eletrophoresis를 이용하여 측정한 결과 60,000 kDa 이었다. 용균 효소의 최적 반응 시간은 20분이었으며 최적 온도는 4$0^{\circ}C$, 최적 pH는 3.0이었다. 온도 안정성은 각 온도에서 30분간 처리하였을 때 $30^{\circ}C$까지는 안정하였으나 4$0^{\circ}C$에서는 80% 활성을 나타내었다. 효소의 pH 안정성은 실온에서 1시간 처리하였을 때 pH 4~7에서 안정성을 나타내었다.

Purification and characterization of enzyme property of a cell-wall lytic enzyme against Lactobacillus plantarum were carried out. Final specific activity of purified enzyme was 5.8 units/mg and purity of the enzyme was increased 8.3 fold compared with the enzyme activity in culture broth. The molecular weight of purified enzyme was estimated to be 60,000 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. Optimal pH and temperature for the activity of this enzyme were 3.0 and 4$0^{\circ}C$, respectively. The cell-wall lytic enzyme activity was maintained at 3$0^{\circ}C$ when treating the enzyme for 30 mins, whereas the activity was decreased to 80% of the maximum level at 4$0^{\circ}C$ The enzyme activity exhibited good stability at the range of pH 4~7.

키워드

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