Utilization of lacZ to Isolate Regulatory Genes from Corynebacterium glutamicum

  • KIM, HYUNG-JOON (Graduate School of Biotechnology, Korea University) ;
  • JOON-SUNG PARK (Graduate School of Biotechnology, Korea University) ;
  • HEUNG-SHICK LEE (Graduate School of Biotechnology, Korea University) ;
  • YOUNHEE KIM (Department of Oriental Medicine, Semyung University)
  • Published : 2002.04.01

Abstract

A total of 100 Corynebacterial clones exerting a regulatory effect on the aceB promoter of Corynebacterium glutamicum were isolated by utilizing a reporter carrying the enteric lacZ gene fused to the promoter. The isolated clones were classified into 3 groups of A, B, and C, according to their color of colonies. Escherichia coli cells carrying clones in groups A and B showed a $90\%\;and\;50\%$ reduction in ${\beta}$-galactosidase activity, respectively. The introduction of group A clones into C. glutamicum also resulted in an almost complete reduction in the expression of the aceA and aceB genes, suggesting that the clones express repressor-like proteins for the genes. Although white colonies were formed on plates containing X-gal, E. coli cells carrying one of the clones in group C exhibited intact ${\beta}$-galactosidase activity. The result suggests that the clone may encode proteins that prevent the cells from accumulating the chromogenic compound, X-gal.

Keywords

References

  1. Chung, T., D. J. Klumpp, and D. C. LaPorte. 1988. Glyoxylate bypass operon of Escherichia coli: Cloning and determination of the functional map. J. Bacteriol. 170: 386-392
  2. Follettie, M. T., O. Peoples, C. Agoropoulou, and A. J. Sinskey. 1993. Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon. J. Bacteriol. 175: 4096-4103
  3. Hong, S. H. and S. Y. Lee. 2000. Metabolic flux distribution in a metabolically engineered Escherichia coli strain producing succinic acid. J. Microbiol. Biotechnol. 10: 496-501
  4. Hwang, B.-J., Y. Kim, H.-B. Kim, H.-J. Hwang, J.-H. Kim, and H.-S. Lee. 1999. Analysis of Corynebacterium glutamicum methionine biosynthetic pathway: Isolation and analysis of metB encoding cystathionine $\beta$-synthase. Mol. Cells 9: 300- 308
  5. Jang, K. H., P. J. Chamber, U.-H. Chun, and M. L. Britz. 2001. Characterization of the cell-surface barriers to plasmid transformation in Corynebacterium glutamicum. J. Microbiol. Biotechnol. 11: 294-301
  6. Jetten, M. S. M., M. E. Gubler, S. H. Lee, and A. J. Sinskey. 1994. Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum. Appl. Environ. Microbiol. 60: 2501-2507
  7. Kinoshita, S. 1985. Glutamic acid bacteria, pp. 115-142. In A. L. Demain and N. A. Solomon (eds.), Biology of Industrial Microorganisms. Benjamin and Cummings Publishing Company, London
  8. Lee, H.-S. and A. J. Sinskey. 1994. Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum. J. Microbiol. Biotechnol. 4: 256-263
  9. Malumbres, M. and J. F. Martin. 1996. Molecular control mechanisms of lysine and threonine biosynthesis in amino acid-producing corynebacteria: Redirecting carbon flow. FEMS Microbiol. Lett. 143: 103-114
  10. Nikaido, H. 1994. Prevention of drug access to bacterial targets: Permeability barriers and active efflux. Science 264: 382-388
  11. Reinscheid, D. J., B. J. Eikmanns, and H. Sahm. 1994. Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme. J. Bacteriol. 176: 3474-3483
  12. Reinscheid, D. J., B. J. Eikmanns, and H. Sahm. 1994. Malate synthase from Corynebacterium glutamicum: Sequence analysis of the gene and biochemical characterization of the enzyme. Microbiology 140: 3099-3108
  13. Sahm, H., L. Eggeling, B. Eikmanns, and R. Krämer. 1995. Metabolic design in amino acid producing bacterium Corynebacterium glutamicum. FEMS Microbiol. Rev. 16: 243-252
  14. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., U.S.A
  15. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96
  16. Sunnarborg, A., D. Klumpp, T. Chung, and D. C. LaPorte. 1990. Regulation of the glyoxylate bypass operon: Cloning and characterization of iclR. J. Bacteriol. 172: 2642-2649
  17. Wendisch, V. F., M. Spies, D. J. Reinschied, S. Schnicke, H. Sahm, and B. J. Eikmanns. 1997. Regulation of acetate metabolism in Corynebacterium glutamicum: Transcriptional control of the isocitrate lyase and malate synthase genes. Arch. Microbiol. 168: 262-269
  18. Wittmann, C., T. Yang, I. Kochems, and E. Heinzle. 2001. Dynamic respiratory measurements of Corynebacterium glutamicum using membrane mass spectrometry. J. Microbiol. Biotechnol. 11: 40-49
  19. Yoo, J.-S., H.-S. Kim, S.-Y. Chung, and Y.-L. Choi. 2000. Molecular characterization of crp, the cyclic AMP receptor protein gene of Serratia marcescens KTCC 2172. J. Microbiol. Biotechnol. 10: 670-676