Journal of Microbiology and Biotechnology

  • 제12권1호
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  • Pages.89-95
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  • 2002
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Adenovirus-Mediated Antisense Expression of Telomerase Template RNA Induces Apoptosis in Lung Cancer Cells

  • Song, Joon-Seok (Graduate School of Biotechnology, Korea University) ;
  • Kim, Sang-Bae (Department of Obstetrics and Gynecology, Korea University) ;
  • Lee, Young-Ho (Department of Obstetrics and Gynecology, Korea University) ;
  • Lee, Kyu-Wan (Department of Obstetrics and Gynecology, Korea University) ;
  • Jung, Hak-Huyn (Department of Otolaryngology, Anam Hospital, Korea University) ;
  • Kim, Mee-Hye (Sewha Pediatric Clinic) ;
  • Kim, Kyung-Tai (Department of Obstetrics and Gynecology, Hanyang University) ;
  • Brown, Robert (CRC Dept. of Medical Oncology, University of Glasgow Beatson Labs) ;
  • Kim, Young-Tae (Graduate School of Biotechnology, Korea University,Department of Obstetrics and Gynecology, Korea University)
  • 발행 : 2002.02.01
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초록

Telomerase is a ribonucleoprotein complex, whose function is to add telomeric repeats $(TTAGGG)_n$ to chromosomal ends and is also known to play an important role in cellular immortalization. Telomerase is highly active in most tumor cells, yet not in normal cells. Therefore, it may have possible applications in cancer gene therapy. Telomerase consists of two essential components; a telomerase RNA template (hTR) and a catalytic subunit (hTERT). The current study attempted to inhibit the "open" part of the human telomerase RNA (hTR) with an antisense sequence-expressing adenovirus. It was found that the antisense telomerase adenovirus suppressed the telomerase activity, tumor cell growth, and survival in vitro. Furthermore, FACS analysis and TUNEL assay suggested that the reduce viability was mediated through the induction of apoptosis, indicating that this approach might be a useful method for suppressing cancer growth in targeted cancer gene therapy.

키워드

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