Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effects of Polymerized Basic Amino Acids Under 50mer Range of Degree of Polymerization on Physiological and Stimulated Mucin Release from Cultured Hamster Tracheal Surface Epithelial Cells

중합도 50mer 이하의 염기성 아미노산 중합체들이 일차배양 햄스터 기관표면 상피세포에서의 생리적 뮤신유리 및 분비자극 상태에서의 뮤신유리에 미치는 영향

  • 이충재 (충남대학교 의과대학 약리학 교실) ;
  • 이재흔 (충남대학교 의과대학 약리학 교실) ;
  • 석정호 (충남대학교 의과대학 약리학 교실) ;
  • 허강민 (충남대학교 의과대학 약리학 교실)
  • Published : 2002.09.01
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Abstract

In the present study, we tried to investigate whether polymerized basic amino acid e.g. poly-L-lysine (PLL) which has the degree of polymerization under 50mer significantly affects the physiological and stimulated mucin release from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with $^3{H}$-glucosamine for 24 hr and chased for 30 min in the presence of either PLLs or adenosine triphosphate (ATP) and PLL to assess the effects on basic or ATP-stimulated $^3{H}$-mucin release. Possible cytotoxicities of PLLs were assessed by measuring lactate dehydrogenase (LDH) release from HTSE cel1s during treatment. The results were as follows: PLLs significantly inhibited basic mucin release from cultured HTSE cells in a dose-dependent manner from the range of 46mer to 14mer; PLL 46mer significantly inhibited the stimulated mucin release by ATP from cultured HTSE cells; there was no significant release of LDH from cultured HTSE cells during treatment. We conclude that PLLs inhibit both physiological and stimulated mucin release from airway epithelial cells without significant cytotoxicity and PLL lost its activity under the range of 14mer. This finding suggests that polymer of basic amino acid like PLL might function as a regulator for hypersecretion of mucus manifested in various respiratory diseases.

Keywords

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