The Stability of Water-soluble and Fat-soluble vitamin in milk by Heat treatments

수용성비타민과 지용성비타민의 가열에 대한 안정성

  • 허정윤 (서울대학교 생활과학대학 식품영양학과) ;
  • 황인경 (서울대학교 생활과학대학 식품영양학과)
  • Published : 2002.10.01

Abstract

This study was conducted to investigate the thermal stability of water-soluble and fat-soluble vitamins dissolved in water and milk by various heat treatments. Vitamin samples were prepared by dissolving them in water and milk at various concentrations, and were heat treated for 30 min at 65$\^{C}$, 15 sec at 85$\^{C}$, 5 sec at 100$\^{C}$, 121$\^{C}$ at 15 min, the levels of residual vitamin were measured by using HPLC. Milk samples were fortified with vitamins before and after UHT treatment. As heating over 100$\^{C}$, riboflavin in water were destructed more than 92% but fortified in milk showed less than 20% destruction, suggesting that riboflavin was protected by milk components. Also retinol heated ever 100$\^{C}$ was more stable in milk than in water. L-Ascorbic acid and cholecalciferol(D$_3$) showed a similar destruction rate in water and in fortified milk. L-ascorbic acid was easily destructed by UHT treatment. Destruction of thiamin and tocopherol was increased in fortified milk. Among tour capsulated water-soluble vitamins, L-ascorbic acid was much more stable compared with powder form. Nicotinic acid and folic acid either in capsule or powder form showed a slight destruction by heat treatment. The results suggested that the fortification of unstable vitamins such as L-ascorbic acid, thiamin, tocopherol and cholecalciferol(D$_3$) should be made in milk after heat treatment.

Keywords

References

  1. Jiri Davidek, Jan Vel:sek and Jan Pokorny : Chemical changes during food processing. Amsterdam-New York-Elsevier 1990, 1990
  2. R Gatti, MG Gioia and V Cavrini : Analysis and stability study of retinoids in pharmaceuticals by LC with fluorescence detection. J. Pharm. Biomed. Anal., 23:147, 2000 https://doi.org/10.1016/S0731-7085(00)00285-5
  3. Soledad Albala-Hurtado, M. Teresa Veciana-Nogues, Maria Izquierdo-Pulico and Abel Marine-Font : Determination of water-soluble vitamins in infant milk by high-performance liquid chromatography. J. Chromatogr. A, 778:247, 1997 https://doi.org/10.1016/S0021-9673(97)00387-7
  4. P Salo-Vaananen, V Ollilainen, P Mattila, K Lehikoinen, E Salmela-Molsa and V. Piironen : Simultaneous HPLC analysis of fat-soluble vitamins in selected animal products after sma11-sca1e extraction. Food Chemistry, 71(4):535, 2000 https://doi.org/10.1016/S0308-8146(00)00155-2
  5. H Qian and M Sheng : Simultaneous determination of fat-soluble vitamins A, D and E and pro-vitamin D2 in animal feeds by one-step extraction and high-perfonnance liquid chromatography analysis. J. Chromatogr. A, 825:127, 1998 https://doi.org/10.1016/S0021-9673(98)00733-X
  6. MM Delgado Zamarreno, A Sanchez Perez, M SanchezRodriguez, MC Gomez Perez and J Hernandez Mendez Determination of fat-soluble vitamins in yogurt by HPLC with electrochemical de:ection. Talanta, 43(9):1555, 1996 https://doi.org/10.1016/0039-9140(96)01939-X
  7. In, YM, Jeong, SG, Ham, JS and Kim, HU : Effect of Heat Treatment on the Milk Quality. RDA. J. Livestock Sci., 40(2):120, 1998
  8. Kim, SS : Changes in Chemical Components of Milk during Microwave HTST Pasteurization. Korean J. of Food Science and Technology, 31(6):1518, 1999
  9. 박성진, 이희덕, 김소희, 정철원, 권태붕 : HPLC를 이용한 수용성 비타민의 동시 분석. 한국식품과학회 창립 30주년 기념 심포지움 제 61차 학술발표회 및 정기총회, 401, 1998
  10. Hiroshi Iwase : Determination of vitamin $D_2$ in emulsified nutritional supplements by solid-phase extraxtion and column-switiching high-performance liquid chromatography with UV detection. J. Chromatogr. A, 881:189, 2000 https://doi.org/10.1016/S0021-9673(00)00198-9
  11. A Escriva, MJ Esteve, R Farre, A Frigola : Determination of liposoluble vitamins in cooked meals, milk and milk products by liquid chromatography. J. Chromatogr. A, 947:313, 2002 https://doi.org/10.1016/S0021-9673(01)01618-1
  12. Shelly A Renken and Joseph J Warthesen : Vitamin D Stability in Milk. J. Food Sci., 58(3), 1993
  13. Panfili, G, Manzi, P and Piazzoferrato, L : Influence of thermal and other manufacturing stresses on retinol isomerization in milk and dairy products. The Journal of Dairy Research 65(2), 1998
  14. GFM Ball : Water-soluble vitamin assays in human nutrition. Chapman & Hall, 1994
  15. Charlotta Turner and Lennart Mathiasson : Determination of vitamins A and E in milk powder using supercritical fluid extraction for sample clean-up. J. Chromatogr. A, 874:275, 2000 https://doi.org/10.1016/S0021-9673(00)00096-0
  16. Renner, Edmund : Milk and dairy products in human nutrition. Hyoil Publications, 1998
  17. Kim, JW : Experimental Studies on the Optimum Pasteurization Condition of the Cow's Milk Produced in Korea. 1. Chemical Composition and Microbiological Aspects of Raw Milk. Res. Rep. Agri. Sci. Tech.Chungnam Nat'l Univ., 14(2), 1987