Restorative Dentistry and Endodontics

  • 제27권1호
  • /
  • Pages.54-65
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  • 2002
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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감염근관에서 Black-Pigmented Bacteria의 동정에 대한 연구

DETECTION OF BLACK-PIGMENTED BACTERIA IN INFECTED ROOT CANALS

  • 권은경 (원광대학교 치과대학 치과보존학교실) ;
  • 김은숙 (원광대학교 치과대학 치과보존학교실) ;
  • 곽주석 (원광대학교 치과대학 치과보존학교실) ;
  • 이황 (원광대학교 치과대학 치과보존학교실) ;
  • 이수종 (원광대학교 치과대학 치과보존학교실) ;
  • 임미경 (원광대학교 치과대학 치과보존학교실)
  • Kwon, Eun-Kyoung (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Kim, Eun-Sook (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Kwak, Ju-Seog (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Lee, Hwang (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Lee, Su-Jong (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Im, Mi-Kyung (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
  • 발행 : 2002.01.01
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초록

Black-pigmented bacteria have been implicated in the endodontic infections. This group of microorganisms includes Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. The organisms display a wide variety of virulence factors that may be pertinent to acute endodontic infections. The aim of this study was to identify P. endodontalis, P. gingivalis, P. intermedia, and P. nigrescens by using special potency disk test, filter paper spot test, 165 rRNA gene-directed PCR, and API 32A. Microbial samples were collected from root canals of 33 intact teeth with necrotic pulp and/or apical periodontitis. Conventional laboratory methods were used for identification of the strains of black pigmented bacteria. Eighteen of 33 samples were positive for the growth of black-pigmented bacteria Five colonies were cultured from each pure cultured colonies from Brucella agar plate. Seventy seven colonies were positive for the growth of black-pigmented bacteria. Thirty three of 77(42.6%) were identifed as P. nigrescens, 10 of 77(12.9%)were P. gingivalis, 6 of 77(7.8%) were P. endodontalis, 10 of 77(12.9%) were P. intermedia. On the contrary the reference strains of P. nigrescens, experimental strains of P nigrescens was sensitive to kanamycin in special potency disk test. 165 rRNA gene PCR and API test after rapid presumptative identification methods, such as special potency disk test and filter paper spot test, would be accurate detection methods for black-pigemented bacteria.

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참고문헌

  1. Kakehashi S, Stanley HR, Fitzgerald RJ.: The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg 20: 340-349, 1965 https://doi.org/10.1016/0030-4220(65)90166-0
  2. Brook I, Frazier EH, Gher ME.: Aerobic and anaerobic microbiology of periapical abscess. Oral Microbiol Immunol 6: 123-125, 1991 https://doi.org/10.1111/j.1399-302X.1991.tb00464.x
  3. Zavistoski J. Dzink J. Onderdonk A. Bartlett J.: Quantitative bacteriology of endodontic infections. Oral Surg 49: 171-174.1980 https://doi.org/10.1016/0030-4220(80)90312-6
  4. Winkelhoff V, Steenbergen TJM, Graaff J.: The role of black-pigmented Bacteroides in human oral infections. J Clin Periodontol Ifi: 145-155.1998
  5. Winkelhoff AJ. Steenbergen TJM. Kippuw N, Graaff J.: Further characterization of Bacteroides endodontalis, an asaccarolytic black-pigmented bacteroides species from the oral S Cavity. J Clin Microbiol 22: 75-79, 1985
  6. Oliveira JCM, Siqueira JF, Alves GB, Hirata R, Andrade AFE.: Detection of Porpltyromonas endodontalis in infected root canals by 16S rRNA gene-directed polymerase chain reaction. J Endodon 26: 729-732. 2000 https://doi.org/10.1097/00004770-200012000-00016
  7. Hofstad T.: Pathogenicity of anaerobic gram-negative rods: possible mechanisms. Rev Infect Dis 6: 189-199, 1984 https://doi.org/10.1093/clinids/6.2.189
  8. Lamont RJ. Jenkinson HF.: Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis. Microbiol Mol Biol Rev 62: 1244-1257. 1998
  9. Shah HN, Collis MD.: Proposal for reclassification of Bacteroides asaccarolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus. Porphyromonas, lnt J Syst Bacteriol 38: 128-131. 1988 https://doi.org/10.1099/00207713-38-1-128
  10. Shah HN. Collins MD.: Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int J Syst Bacteriol 40: 205-208, 1990 https://doi.org/10.1099/00207713-40-2-205
  11. Winkelhoff AJ, Steenbergen TJM, Graaff J.: Porphyromonas (Bacteroides) endodontalis: Its role in endodotal infections. J Endodon 18: 431-433. 1992 https://doi.org/10.1016/S0099-2399(06)80843-5
  12. Steenbergen TJM, Winkelhoff AJ, Grenier D, Graff J.: Bacteroides endodontalis sp. nov.. an Asaccharolytic black-pigemented bacteroides species from infected dental root canals. Int J Syst Bacteriol 2: 118-120. 1984
  13. Slots J. Genco RJ.: Direct hemagglutination technique for differentiating Bacteroides asaccarolyticus oral strains from nonoral strains. J Clin Microbiol 10: 371-374, 1979
  14. Herweijer JA. Loos BG, Neiders ME.: Characterization of total membrane proteins of Porphyromonas endodontalis. J Endodon 18: 620-624. 1992 https://doi.org/10.1016/S0099-2399(06)81334-8
  15. Stubbs S. Park SF. Bishop PA. Lewis MAO.: Direct detection of Preootella intermedia and P. nigrescens in suppurative oral infection by amplication of 16S rRNA gene. J Med Microbiol 18: 1017-1022, 1999
  16. Gharbia SE. Haapasalo M. Shah HN, Kotiranta A, Lounatmaa K. Pearce MA, Devine DA.: Characterization of Prevotella intermedia and Prevotella nigrescens isolates from periodontic and endodontic infections. J periodontol 65: 56-61. 1994 https://doi.org/10.1902/jop.1994.65.1.56
  17. Shah HN, Gharbia SE.: Biochemical and chemical studies on strains designated Preootella intermedia and proposal of a new pigmented species. Preootella nigrescens sp. nov. Int J Syst Bacteriol 42: 542-546, 1992 https://doi.org/10.1099/00207713-42-4-542
  18. Nisengard & Newman.: Oral microbiology and immunology. second edition. 213-216. 1991
  19. Baumgartner JC. Watkins BJ. Bae KS, Xia T.: Association of black-pigmented bacteria with endodontic infections. J Endodon 25: 413-417, 1999 https://doi.org/10.1016/S0099-2399(99)80268-4
  20. Bae KS. Baumgartner JC. Shearer TR, David LL.: Occurence of Prevotella nigrescens and Prevotealls intermedia in infections of Endodontic Origin. J Endodon 23: 620-623. 1997 https://doi.org/10.1016/S0099-2399(97)80173-2
  21. Sundqvist G, Johnasson E, Sjogren D.: Prevalence of black-pigmented bacteroides species in root canal infections. J Endodon 15: 13-18, 1989 https://doi.org/10.1016/S0099-2399(89)80092-5
  22. Siqueira JF, Rocas IN, Oliveira JCM, Santos KRN.: Detection of putative oral pathogens in acute periradicular abscesses by 16S rDNA-directed polymerase chain reaction. J Endodon 27: 164-167, 2001 https://doi.org/10.1097/00004770-200103000-00004
  23. Siqueria JF, Rocas IN, Oliveira JCM, Santos KRN.: Molecular detection of black-pigmented bacteria in infections of endodontic origin. J Endodon 27: 563-566, 2001 https://doi.org/10.1097/00004770-200109000-00002
  24. 김양호, 김선희.: 진단 임상 미생물학. 284-406, 2000
  25. Haapasalo M.: Bacteroides spp. in dental root canal infections. Endod Dent Traumatol. 5: 1-10, 1989 https://doi.org/10.1111/j.1600-9657.1989.tb00330.x
  26. Bogen G.. Slots J.: Black-pigmented anaerobic rods in closed periapical lesions. Int Endodon J 32: 204-210. 1999 https://doi.org/10.1046/j.1365-2591.1999.00216.x
  27. Slots J, Ashimoto A. Flynn MJ.: Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplication with the polymerase chain reaction. Clin Infect Dis 20: S304-7,1995 https://doi.org/10.1093/clinids/20.Supplement_2.S304
  28. Steenbergen TJM, Menard C, Tijhof CJ, Mouton C, Graaff J:. Comparison of three molecular typing methods in studies of transmission of Porphyronas gingivalis. J Med. Micobiol 14: 416-421. 1993
  29. Summanen P, Baron EJ, Citron DM, Strong CA, Wexler HM, Finegold SM.: Wadsworth Anaerobic bacteriology Manual. 1993
  30. Moncla BJ, Braham P.: Detection of sialidase (Neuraminidase) activity in actinomyces species by using 2' -(4-Methylumbelliferyj)$\alpha$-D-N-Acetylneuraminic acid in a filter paper spot test. J Clin Microbiol 27: 182-184,1989
  31. Moncla BJ, Braham P, Rabe LK. Hillier SL.: Rapid presumptive identification of black-pigmented gramnegative anaerobic bacteria by using 4-methylumbelliferone derivatives. J Clin Mirobiol 29: 1955-1958, 1991
  32. Slots J.: Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system, J Clin Microbiol 14: 288-294, 1981
  33. Wayman BE, Murata SM, Almeida RJ, Fowler CB.: A bacteriological and histological evaluation of 58 periapical lesions. J Endodon 18: 152-155, 1992 https://doi.org/10.1016/S0099-2399(06)81409-3
  34. Matto J, Asikainen S, Vaisanen ML, Rautio M, Saarela M, Summanen P, Finegold S, Jousimies-Somer H.: Role of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens in extraoral and some odontogenic infections. Clin Infect Dis 25: S194-8, 1997 https://doi.org/10.1086/516205