Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Green Tea (-) Epigallocatechin-gallate Induces the Apoptotic Death of Prostate Cancer Cells

녹차 (-)Epigallocatechin-gallate에 의한 전립선암 세포주 DU145 세포고사 기전

  • 이지현 (원광대학교 의과대학 미생물학교실) ;
  • 정원훈 (원광보건대학) ;
  • 박지선 (전북대학교 식품영양학과) ;
  • 신미경 (원광대학교 식품영양학과) ;
  • 손희숙 (전북대학교 식품영양학과) ;
  • 박래길 (원광대학교 의과대학 미생물학교실)
  • Published : 2002.06.01
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Abstract

The mechanism by which catechin-mediated cytotoxicity against tumor cells remains to be elusive. To elucidate the mechanical mights of anti-tumor effects, (-)epigallocatechin-gallate (EGCG) of catechin was applied to human prostate cancer DU 145 cells. Cell viability was measured by crystal violet staining. Cell lysates were wed to measure the catalytic activity of caspases by using fluorogenic peptide: Ac-DEVD-AMC for caspase-3 protease, Z-IETD-AFC for caspase-8 protease, Ac-LEHD-AFC for caspase-9 protease as substrates. The equal amounts of protein from cell lysate was separated on SDS-PAGE and analyzed by western blotting with anti-Fas antibody, anti-FasL antibody, anti-BCL2 antibody and anti-Bax antibody. (-)EGCG induced the death of DUl45 cells, which was revealed as apoptosis shown by DNA fragmentation. (-)EGCG induced the activation of caspase family cysteine proteases including caspase-3, -8 and -9 proteases in DU145 cells. Also, (-)EGCG increased the expression of Fas and Fas ligand (FasL) protein in DU145 colls. The expression level of BCL2 was decreased in (-)EGCG treated DU145 cells, whereas Bax protein was increased in a time-dependent manner. We suggest that (-)EGCG-induced apoptosis of DU145 cells is mediated by signaling pathway involving caspase family cysteine protease, mitochondrial BCL2-family protein and Fas/FasL.

Keywords

References

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