The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

Detection and Distribution of Apple scar skin viroid-Korean Strain (ASSVd-K) from Apples Cultivated in Korea

  • Lee, Jai-Youl (Department of Microbiology, Kyungpook National University) ;
  • Kwon, Mi-Jo (Department of Microbiology, Kyungpook National University) ;
  • Hwang, Seung-Lark (Department of Microbiology, Kyungpook National University) ;
  • Lee, Sung-Joon (Daegu Apple Research Institute, Rural Development Adminstration) ;
  • Lee, Dong-Hyuk (Department of Microbiology, Kyungpook National University)
  • 발행 : 2002.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Apple scar skin viroid (ASSVd) has been one of the most destructive diseases in Korean apple orchards. Symptoms of the scar skin viroid disease were detected in various apple cultivars, namely, Sansa, Fuji, Chukwang, Miki-Life, Hongro, and Songbongeum cultivated in the southern part of Korea. The RNA molecules were extracted from the apples bearing dapple apple symptoms with the application of CF-11 RNA extraction method. The purified RNAs were used for the synthesis of cDNA with RT-PCR. The PCR products were cloned and sequenced. The viroid RNA molecules from the six different cultivars bearing the dapple symptos showed the same nucleotide sequences as that of the Korean strain of ASSVd（ASSVd-K). ASSVd-K was detected from apple orchards in Kunwi, Sangju, Uiseong, Yeong-yang, Andong, and Youngduk in Gyeongbuk Province in 2001, and in Muju in Jeonbuk Province in 2002. As the viroid disease could be propagated vegetatively, it can be widely transmitted gradually in Korea.

키워드

참고문헌

  1. Diener, T. 0. 1987. The Viroids. Plenum Press: New York and London
  2. Di Serio, F., Apahcio, F., Alioto, D., Ragozzino, A. and Flores, R. 1996. Identification and molecular properties of a 306 nucleotide viroid associated with apple dimple fruit disease. J. Gen. Virol. 77:2833-2837 https://doi.org/10.1099/0022-1317-77-11-2833
  3. Flores, R., Randles, J. W., Bar-Joseph, M. and Diener, T. 0. 1998. A proposed scheme for viroid classification and nomenclature. Arch. Virol. 143:623-629 https://doi.org/10.1007/s007050050318
  4. Hadidi, A., Huang, C., Hammond, R. W. and Hashimoto, J. 1990. Homology of the agent associated with dapple apple disease to apple scar skin viroid and molecular detection of these viroids. Phytopathology 80:263-268 https://doi.org/10.1094/Phyto-80-263
  5. Hashimoto, J. and Koganezawa, H. 1987. Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Res. 15:7045-7052 https://doi.org/10.1093/nar/15.17.7045
  6. Hurtt, S. S., Podleckis, E. V. and Howell, W. E. 1996. Integrated molecular and biological assays for rapid detection of apple scar skin viroid in pear. Plant Dis. 80:458-462 https://doi.org/10.1094/PD-80-0458
  7. Lee, J. H., Park, J. K., Lee, D. H., Uhm, J. Y., Ghim, S. Y. and Lee, J. Y. 2001. Occurrence of Apple scar skin viroid-Korea strain (ASSVd-K) in Apple Cultivated in Korea. Plant Pathol J. 17:300-304
  8. Pallas, V., Navarro, A. and Flores, R. 1987. Isolation of a viroidlike RNA from hop different from hop stunt viroid. J. Gen. Virol. 68:3201 -3205 https://doi.org/10.1099/0022-1317-68-12-3201
  9. Puchta, H., Luckinger, R., Yang, X., Hadidi, A. and Sanger, H. L. 1990. Nucleotide sequence and secondary structure of apple scar skin viroid (ASSVd) from China. Plant Mol. Biol. 14:1065-1067 https://doi.org/10.1007/BF00019406
  10. Sanger, H. L. and Lee, J. Y. 1993. Molecular biology of the viroids, the smallest pathogens presently known. Korean J. Plant Pathol. 9:139-148
  11. Sano, T., Hataya, T., Terai, Y. and Shikata, E. 1989. Hop stunt viroid strains from dapple fruit and peach in Japan. J. Gen. Virol. 70:1311-1319 https://doi.org/10.1099/0022-1317-70-6-1311
  12. Yan, F., Wah, W. C. and Symons, R. H. 1997. A high sensitivity RT-PCR assays for the diagnosis of grapevine viroids in field and tissue culture samples. J. Virol. Methods 63:57-69 https://doi.org/10.1016/S0166-0934(96)02115-5

피인용 문헌

  1. Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea vol.21, pp.4, 2015, https://doi.org/10.5423/RPD.2015.21.4.346
  2. Transmission of Apple scar skin viroid by Grafting, Using Contaminated Pruning Equipment, and Planting Infected Seeds vol.22, pp.1, 2006, https://doi.org/10.5423/PPJ.2006.22.1.063
  3. Efficiency of virus elimination in apple calli (cv. Hongro) derived from meristem culture of dormant buds vol.44, pp.4, 2017, https://doi.org/10.5010/JPB.2017.44.4.379
  4. Evaluation of phenolic compounds from viroid-free and viroid-infected apples using HPLC-PDA-ESI-MS/MS pp.09580344, 2019, https://doi.org/10.1002/pca.2822