The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and Characterization of a Ringspot Isolate of Odontoglossum ringspot virus from Cymbidium var.'Grace Kelly'

  • Park, Won-Mok (Graduate School of Biotechnology, Korea University) ;
  • Park, Seung-Kook (Plant Virus GenBank, Division of Life and Environmental Sciences, Seoul Women’s University) ;
  • Park, Sun-Hee (Plant Virus GenBank, Division of Life and Environmental Sciences, Seoul Women’s University) ;
  • Ryu, Ki-Hyun (Division of Biomedical Science, ai Chai University) ;
  • Park, Chang-Won (Division of Biological Environment, Kangwon National University) ;
  • Park, Jang-Kyung (Graduate School of Biotechnology, Korea University)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

An isolate of Odontoglossum ringspot virus (ORSV) was identified from Cymbidium var. 'Grace Kelly' showing ringspot symptom on the floral and leaf parts, and was denoted as cymbidium ringspot isolate (ORSV-CR). In ultrathin sections of leaf tissue from diseased Cymbidium plants, clusters of virus particles were observed in the vacuole and cytoplasm. In the Western blot hybridization, the virus strongly reacted with ORSV-specific antiserum indistinguishable from ORSV, suggesting that the vims is serologically identical with ORSV. ORSV-CR sap was inoculated onto 20 species belonging to 12 genera. Systemic infection occurred in Cymbidium sp., Nicotiana benthamiana and N. clevelandii, the host of which was found to be different from that of ORSV-Cy, the Korean strain of ORSV. The analysis of coat protein (CP) gene showed that ORSV-CR was highly homologous to the known isolates of ORSV, with over 95.6% identity in amino acid level. Phylogenetic tree analysis of CP showed that ORSV-CR was clustered with the known ORSV isolates, suggesting that ORSV is a very stable tobamovirus.

Keywords

References

  1. Ajjikuttira, P. A., Lim-Ho, C. L., Woon, M. H., Ryu, K. H., Chang, C. A., Loh, C. S. and Wong, S. M. 2002. Genetic vaiiability in the coat protein genes of two orchid viruses: Cymbidium mosaic virus and Odontoglossum ringspot virus. Arch. Virot. 147:1943-1954 https://doi.org/10.1007/s00705-002-0868-5
  2. Chng, C. G., Wong, S. M., Mahtahi, P. H., Loh, C. S., Goh, C. J., Kao, M. C., Chung M. C. and Watanabe, Y. 1996. The complete sequence of a Singapore isolate of Odontogtossum ringspot virus and comparison with other tobamovimses. Gene 171:155-161 https://doi.org/10.1016/0378-1119(96)00046-7
  3. Chen, Y. K., Derks, A. F. L. M., Langeveld, S., Goldbach, R. and Prins, M. 2001. High sequence conservation among cucumber mosaic vims isolates from lily. Arch. Virot. 146:1631-1636 https://doi.org/10.1007/s007050170085
  4. Choi, S. K., Yoon, J. Y., Ryu, K. H., Choi, J. K. and Park, W. M. 2002. First report of Zucchini yellow mosaic virus on hollyhock (AIthaea rosea). Ptant Pathot. J. 18:121-125 https://doi.org/10.5423/PPJ.2002.18.3.121
  5. Christie, R. G. and Edwardson, J. R. 1977. Light and electron microscopy of plant virus inclusions. Ftorida Agricutturat Experiment Stations Monograph Series No. 9, 150 p., Institute of Food and Agricultural Sciences, University of Flohda, Gainesville, Flonda
  6. Corbett, M. K. 1967. Some distinguishing characteristics of the orchid strain of tobacco mosaic vims. Phytopathotosy 57:164-172
  7. Dawson, W. O. 1992. Tobamovirus-plant interactions. Virotoey 186:359-367 https://doi.org/10.1016/0042-6822(92)90001-6
  8. Dore, I., Dekker, E. I., Porta, C. and Van Regenmortel, M. H. V. 1987. Detection of ELISA of two tobamoviruses in orchids using monoclonal antibodies. J. Phytopathot. 20: 317-326 https://doi.org/10.1111/j.1439-0434.1987.tb00495.x
  9. Edwardson, J. R. and Chhstie, R. G. 1978. Use of virus induced inclusions in classification and diagnosis. Annu. Rev. Phytopathot. 16:31-55 https://doi.org/10.1146/annurev.py.16.090178.000335
  10. Edwardson, J. R. and Zettler, F. W. 1986. Odontogtossum ringspot virus, pp. 233-247, In: van Regenmortel, M. H. V., and Frankel-Conrat, H. (eds.), The Ptant Viruses, VoI. 2. The RodShaped Ptant Viruses, Plenum Publisher, New York
  11. Fraile, A., Escriu, F., Aranda, M. A., Malpica, J. M., Gibbs, J. M. and Garcia-Arenal, F. 1997. A century of tobamovirus evolution in an Australian population of Nicotiana glauca. J. Virol. 71:8316-8320
  12. Gibbs, A. J. 1977. Tobamovirus group. CMI/AAB Descriptions of Ptant Viruses No. 184, Kew, England
  13. Granett, A. L. and Shalla, T. A. 1970. Discrepancies in the intercellular behavior of three strains of tobacco mosaic virus, two of which are serologically indistinguishable. Phytopathotogy 60:419-425 https://doi.org/10.1094/Phyto-60-419
  14. Ikegami, M., Isomura, Y., Matumoto, Y., Chatani, M. and Inouye, N. 1995. The complete nucleotide sequence of Odontoglossum ringspot virus (Cy-l strain) genomic RNA. Microbiol. Immunol. 39:995-1001 https://doi.org/10.1111/j.1348-0421.1995.tb03289.x
  15. Jesen, D. D. and Gold, A. H. 1951. A virus ringspot odontoglossum orchid: symptoms, transmission and electron microscopy. Phytopathotogy 41:648-653
  16. Matthews, R. E. F. 1991. Ptant Virotogy, 3th edition. AcademicPress, San Diego, California.
  17. Milicic, D. and Stefanac, Z. 1971. Cell inclusions of the Cucumber green mottle mosaic virus and the Odontoglossum ringspot virus. Act. Bot. Croat. 30:33-40.
  18. Namba, R. and Ishii, M. 1971. Failure of aphids to transmit the Odontoglossum ringspot and Cymbidium mosaic viruses to orchid plantlets derived from meristem culture. Phytopathot-ew 61:582-583 https://doi.org/10.1094/Phyto-61-582
  19. Park, W. M., Yoon, K. E., Chung, S. Y. and Ryu, K. H. 1990. Identification of Odontoglossum ringspot virus isolated from Cymbidium goeringii Reichenbach in Korea. Korean J. Plant Pathol. 6:387-392
  20. Ryu, K. H., Choi, C. W., Choi, J. K. and Park, W. M. 1995. Clon-ing of the 3'-termina1 region encoding movement and coat pro-terns of a Korean isolate of odontoglossum nngspot virus. Arch. Virol. 140:481-490 https://doi.org/10.1007/BF01718425
  21. Ryu, K. H., Kim, W. K. and Park, W. M. 1994. Ultrastructure of Cymbidium leaf tissue systemically infected with Odontogtos-sum ringspot virus. Korean J. Etectr. Micro. 24:46-54
  22. Ryu, K. H. and Park, W. M. 1995. The complete nucleotide sequence and genome organization of Odontogtossum ring-spot tobamovirus RNA. Arch. Virol. 140:1577-1587 https://doi.org/10.1007/BF01322531
  23. Sambrook, J., Fiitsch, E. F. and Maniads, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Labora-tory, Cold Spiing Harbor, New York
  24. Toussaint, A., Dekegel, D. and Vanheule, G. 1984. Distribution of Odontogtossum ringspot virus in apical menstem of infected Cymbidium cultivars. Physiol. Plant Pathol. 25:297-305 https://doi.org/10.1016/0048-4059(84)90037-7
  25. Zaitlin, M. 1954. Detection of virus in Catttew and Cymbidium orchids by serological method. Phytopathotogy 44:314-318