Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Genetic Modification of Coffee Plants

  • Shinjiro Ogita (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Hirotaka Uefuji (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Park, Yong-Eui (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Tomoko Hatanaka (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Mikihiro Ogawa (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Yube Yamaguchi (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Nozomu Koizumi (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan) ;
  • Hiroshi Sano (Plant Molecular Breeding, Research and Education Center for Genetic Information, Nara institute of Science and Technology, Japan)
  • 발행 : 2002.09.01
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초록

An efficient molecular breeding technique for coffee plants was developed. In order to produce transgenic coffee plants, we established a model transformation procedure via Agrobacterium method. We isolated a gene encoding a protein possessing 7-methylxanthine methyltransferase (theobromine synthase) activity, and it was designated as Coffea arabica 7-methylxanthine methyl transferase; CaMXMT. Using this clone, we produced transgenic coffee plants, in which the expression of CaMXMT is suppressed by double-stranded RNA interference (RNAi) andlor anti-sense methods. The expression pattern of CaMXMT was analyzed by reverse transcription-PCR method and we found that, in the transformed cell lines, the level of transcripts were obviously suppressed by RNAi. The endogenous level of caffeine in the transformed cells was dramatically reduced in comparison with non-transformed cells.

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참고문헌

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