Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Screening of Soybean Recombinant Inbred Lines for High Competence Somatic Embryogenesis

대두 재조합순계주에서 고빈도 체세포배발생능 계통 조사

  • Choi, Pil-Son (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Takao Komatsuda (Department of Gene Diversity, National Institute of Agrobiological Resources (NIAS)) ;
  • Kim, Min-Hoon (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Kyu-Myeong (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Dong-Woog (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Jang-Ryol (Eugentech Inc., Korea Research Institute of Bioscience and Biotechnology (KRIBB), Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 2002.06.01
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Abstract

Cotyledonary explants from immature zygotic embryos of each 85 recombinant inbred lines (RILs) were cultured on medium containing MS salts, B5 vitamins, 40 mg$^{-1}$ 2,4-dichlorophenoxyacetic acid and 30 g$^{-1}$ sucrose. Frequency of somatic embryo formation on cotyledonary explants showed in thirty-six lines(＜10%), in thirty-seven lines (11~49%), in nine lines (50~89%), and in three lines(>90%), respectively, The highest frequency (up to 90%) and number (6.36 per cotyledon) of somatic embryos were obtained from lines of KM1010, KM1032 and KM1064. Primary somatic embryos produced from three lines produced numerous secondary somatic embryos on the surfaces, which were subcultured for over one year. Upon transfer to maturation and conversion medium (Komatsuda, 1992), somatic embryos converted to plantlets at a frequency of approximately 25%.

대두 85개 재조합순계주의 미숙배로부터 얻은 자엽절편을 MS salt, B5비타민, 40 mg$^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D) 및 30 g$^{-1}$ sucrose로 조성된 체세포배유도배지 (SIM, somatic induce medium)에 치상하여 5주간 배양하였다. 10%이하의 체세포배형성빈도는 36계통에서, 11~49%의 형성빈도는 37계통에서, 50~80%의 형성빈도는 9계통에서 그리고 90% 이상의 형성빈도는 3계통에서 각각 관찰되었다. 90% 이상의 체세포배형성빈도와 자엽당 6.36개의 체세포배를 형성하는 계통으로는 KM1010, KM1032 및 KM1064이었으며, 3계통에서 얻은 일차배를 동일배지상에서 계대배양하면서 2차배 증식방법으로 1년 이상 증식 유지하였다. 체세포배를 성숙배지와 식물체전환배지 (Komatsuda 1992)에 옮겼을 때 약 25% 정도가 정상식물체로 생육하였다.

Keywords

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